VCell, COPASI and SpringSalad were taught and used for projects at the 25th Annual Computational Cell Biology (CCB) workshop that took place online February 26-28th. The invited talks were given by Yulia Timofeeva (University of Warwick), James P Sluka (Indiana University) and Kevin Janes (University of Virginia). Sixteen participants were selected to work with VCell and COPASI instructors on their projects, and eight gave presentations on their projects at the Modeling Symposium.