- Zoomed wide field fluorescent microscopy image of S. aureus stained with Nile Red. (J.Batchelder, Mok Lab)
- Zoomed SIM image of S. aureus stained with Nile Red. (J.Batchelder, Mok Lab)
- Widefield microscope image of mitochondria. (CCAM)
- SIM image of mitochondria. (CCAM)
- Widefield microscope 3D stack of mitochondria. (CCAM)
- SIM 3D stack of mitochondria. (CCAM)
- Zoomed Widefield Image of MAP2 Stained Neuronal cultures. (K. Caro, Martinelli Lab)
- STORM Image of MAP2 Stained Dendrites. (K. Caro, Martinelli Lab)
Super Resolution Techniques
Technique | System | Lateral Resolution | Axial Resolution | Fluorophores |
---|---|---|---|---|
SIM | Widefield | ~120 nm | ~ 250 nm | Standard |
SMLM | Widefield | ~20 nm | ~20 nm | Photoswitchable, Photoactivatable |
STED | Confocal | ~20 nm | ~50 nm | Standard |
SIM - Structured illumination microscopy
SMLM - Single-molecule localization microscopy:
- PALM - Photoactivated localization microscopy
- STORM - Stochastic optical reconstruction microscopy
- PAINT - Point accumulation in nanoscale topography
STED - Stimulated emission depletion
Sample Preparation
Zeiss Elyra 7 Quick Guide - Information pertaining to sample preparations for SIM, PALM, and dSTORM.
Optimizing Imaging Conditions for Demanding Multi-Color Super Resolution Localization Microscopy - article introducing a new SMLM imaging buffer, OxEA, for use in 3-color experiments using Alexa dyes 488, 555 and 647.
Dyes for Single Molecule Localization
abberior CAGE dyes - family of dyes specific for PALM and STORM.
Calibration Tools
GATTA quant - Nanorulers for checking resolution on a SIM system.