Zoomed wide field fluorescent microscopy image of S. aureus stained with Nile Red. (J.Batchelder, Mok Lab)
Super Resolution Techniques
| Technique | System | Lateral Resolution | Axial Resolution | Fluorophores |
|---|---|---|---|---|
| SIM | Widefield | ~120 nm | ~ 250 nm | Standard |
| SMLM | Widefield | ~20 nm | ~20 nm | Photoswitchable, Photoactivatable |
| STED | Confocal | ~20 nm | ~50 nm | Standard |
SIM - Structured illumination microscopy
SMLM - Single-molecule localization microscopy:
- PALM - Photoactivated localization microscopy
- STORM - Stochastic optical reconstruction microscopy
- PAINT - Point accumulation in nanoscale topography
STED - Stimulated emission depletion