Microscopy Equipment

Academic Research Building (ARB)
E6011
263 Farmington Avenue

UConnHealth

Zeiss LSM 880

Zeiss 880 CLSMThe 880 is mounted on an Axio Observer Z1 with automated xyz control. The system is equipped with a spectral 32-channel QUASAR GaAsp detector allowing for spectral imaging and maximum sensitivity photon counting. There are two adjacent PMT's and a transmitted light detector. The accompanying incubation system, which encloses the microscope, offers stable environmental conditions with temperature, humidity and carbon dioxide control.

Lasers Objectives
 405 nm Diode  10x/0.3 EC Plan-Neofluar
 458, 488, and 514 nm Argon  20x/0.8 Plan-Apochromat
 561 nm  Diode Pumped Solid State (DPSS)  25x/0.8 LD LCI Plan-Apochromat, W,Sil,Gly,Oil
 633 nm Helium Neon (HeNe)  40x/1.3 Achrostigmat Oil
 40x/1.2 C-Apochromat Water
 63x/1.4 Plan-Apochromat Oil

Zeiss LightSheet

Zeiss_LightsheetThe Zeiss LightSheet is an advanced fluorescent microscope that allows you to image optical sections of large, live or fixed samples with virtually no bleaching or phototoxicity to the specimen.  The optics are equipped for water-based or cleared specimens and dual sCMOS cooled cameras to provide high sensitivity, rapid, 3D imaging of live or fixed thick specimens. The extremely fast acquisition speeds allow you to capture large amounts of data within a fraction of the time compared to other imaging techniques.

Lasers Emission Objectives Excitation Objectives
 405 nm Diode 5x/0.16 EC Plan-Neofluar 5x/0.1
 488 nm Diode 20x/1.0 Plan-Neofluar Clearing 10x/0.2
 561 nm  Diode 20x/1.0 Plan-Apochromat Water 10x/0.2
 633 nm Diode

Imaging01

User terminalThis CCAM user computer is a Windows 7 computer with MetaMorph, Photoshop, Office, Zeiss LSM and Zen Image Browsers. All the software is available, with prior approval, via remote access.

Cell & Genome Sciences Building (CGSB)
Imaging Suite R1510
400 Farmington Avenue

UConnHealth - CGSB

Zeiss 780

Zeiss 780The 780 is a combined confocal/FCS/NLO system, mounted on an inverted Axio Observer Z1. The 780 employs the spectral 32-channel QUASAR GaAsp detector allowing for spectral imaging, maximum sensitivity photon counting and Fluorescence Correlation Spectroscopy (FCS) as well as two adjacent PMT's. The accompanying incubation system, which encloses the microscope, offers stable environmental conditions with temperature, humidity and carbon dioxide control. This microscope is also equipped with a Becker-Hinkl Fluorescence Lifetime Imaging Microscopy (FLIM) detector on a nondescanned detection port, particularly useful for detection of fluorescence resonance energy transfer (FRET) based probes.

Lasers Objectives
 405 nm and 440 nm Diode  10x/0.45 W C-Apochromat
 458, 488, and 514 nm Argon  20x/0.8 Plan-Apochromat
 561 nm  Diode Pumped Solid State (DPSS)  40x/1.2 W C-Apochromat
 633 nm Helium Neon (HeNe)  40x/1.3 Oil Plan-Neofluar
720 - 950 nm Coherent Chameleon tunable, software controlled, modelocked Ti:Sapphire laser for multi-photon excitation (NLO)  63x/1.4 Plan-Apochromat Oil

Zeiss Confocor 3

Zeiss 780The CC3 is a combined confocal and FCS system, mounted on a AxioVert 200M. This system has two avalanche photodiodes (APD) with ultimate sensitivity allowing for decreased laser power with delicate specimens as well as three traditional PMTs. The APD's are highly efficient in the red wavelength range where fluorescent signal can be very weak.
 
 
 
The following lasers are on the Confocor 3:

  • 458, 488, and 514 nm Argon
  • 561 nm Diode-PUmped Solid State (DPSS)
  • 633 nm Helium Neon (HeNe)

Collaborative PALM and diSPIM

Photo-Activated Localization Microscopy (PALM) super-resolution light microscopy is available through collaboration using a home-built, fully automated PALM imaging system in a CCAM associated laboratory. This system is built on ASI’s custom Rapid Automated Modular Mounting (RAMM) platform, uses 440, 515 and 594 nm (Coherent OBIS) laser lines, and is equipped with Hamamatsu sCMOS cameras for image acquisition.
 
 
diSPIM (dual-view inverted Selective Plane Illumination Microscope) imaging system from ASI imaging Inc is available through collaboration in a CCAM associated laboratory for lightsheet imaging on the cellular as opposed to tissue scale.

Imaris

Imaris User Terminal Imaris is a 64 bit computer with ImarisSurpass 3-D rendering and analysis software, MetaMorph, Photoshop, and Zeiss Zen Image Browser.
All software is available, with prior approval,  via remote access.

Spex

SpexThe Spex is a single excitation, dual emission spectrofluorimeter.

CCAM Wet Lab

Tissue Culture HoodProvides tissue culture hood and tissue culture microscope, incubators, refrigeration, and bench space.