Please refer to the following spectral tools below to see if your probes are compatible with our equipment. If you need further assistance, please contact Susan, email@example.com, 860-679-4686.
- ThermoFisher Scientific Fluorescence SpecrtraViewer
- BioLegend Fluorescence Spectra Analyzer
- FPbase Spectra Viewer
- AAT Bioquest Fluorescence Spectrum Viewer
Zeiss LSM 880
The 880 is mounted on an Axio Observer Z1 with automated xyz control. The system is equipped with a spectral 32-channel QUASAR GaAsp detector allowing for spectral imaging and maximum sensitivity photon counting. This detector is effective from 410 - 696 nm. There are two adjacent PMT's, effective range 415 - 735 nm, and a transmitted light detector. The accompanying incubation system, which encloses the microscope, offers stable environmental conditions with temperature, humidity and carbon dioxide control.
|405 nm Diode||10x/0.3 EC Plan-Neofluar|
|458, 488, and 514 nm Argon||20x/0.8 Plan-Apochromat|
|561 nm Diode Pumped Solid State (DPSS)||25x/0.8 LD LCI Plan-Apochromat, W,Sil,Gly,Oil|
|633 nm Helium Neon (HeNe)||40x/1.3 Achrostigmat Oil|
|40x/1.2 C-Apochromat Water|
|63x/1.4 Plan-Apochromat Oil|
The Zeiss LightSheet is an advanced fluorescent microscope that allows you to image optical sections of large, live or fixed samples with virtually no bleaching or phototoxicity to the specimen. The optics are equipped for water-based or cleared specimens and dual sCMOS cooled cameras to provide high sensitivity, rapid, 3D imaging of live or fixed thick specimens. The extremely fast acquisition speeds allow you to capture large amounts of data within a fraction of the time compared to other imaging techniques.
|Lasers||Emission Objectives||Excitation Objectives|
|405 nm Diode||5x/0.16 EC Plan-Neofluar||5x/0.1|
|488 nm Diode||20x/1.0 Plan-Neofluar Clearing||10x/0.2|
|561 nm Diode||20x/1.0 Plan-Apochromat Water||10x/0.2|
|633 nm Diode|
The 780 is a combined confocal/FCS/NLO system, mounted on an inverted Axio Observer Z1. The 780 employs the spectral 32-channel QUASAR GaAsp detector allowing for spectral imaging, maximum sensitivity photon counting, and Fluorescence Correlation Spectroscopy (FCS) as well as two adjacent PMTs. The GaAsp detector has a detection range of 410 - 696 nm while the PMTs have a range from 415 - 735 nm. The accompanying incubation system, which encloses the microscope, offers stable environmental conditions with temperature, humidity and carbon dioxide control. This microscope is also equipped with a Becker-Hinkl Fluorescence Lifetime Imaging Microscopy (FLIM) detector on a nondescanned detection port, particularly useful for detection of fluorescence resonance energy transfer (FRET) based probes.
|405 nm and 440 nm Diode||10x/0.45 W C-Apochromat|
|458, 488, and 514 nm Argon||20x/0.8 Plan-Apochromat|
|561 nm Diode Pumped Solid State (DPSS)||40x/1.2 W C-Apochromat|
|633 nm Helium Neon (HeNe)||40x/1.3 Oil Plan-Neofluar|
|720 - 950 nm Coherent Chameleon tunable, software controlled, modelocked Ti:Sapphire laser for multi-photon excitation (NLO)||63x/1.4 Plan-Apochromat Oil|
Elyra 7 SIM2 - Coming May 2023
Structured illumination microscopy, SIM, is a widefield technique that greatly increases the diffraction-limited resolution of the light microscope, ~ 200 nm, to super-resolution, ~ 100 nm. SIM uses a periodic grating that is projected onto the image plane of the microscope. Software is then used to analyze the resulting Moiré pattern, and through deconvolution, a super-resolution image is obtained. The Zeiss Elyra 7 SIM2 uses an advanced reconstruction algorithm that improves the SIM resolution to less than 100 nm, along with quick image acquisition and compatibility for live-cell applications.
In addition to SIM, the Elyra is capable of other high-resolution techniques such as TIRF, PALM, and STORM.
Samples live and fixed, up to ~ 150 µm thickness can be viewed on the Elyra 7 SIM2
The Elyra is mounted on a Zeiss Axio Observer 7, with definite focus. The system is equipped with an XY and Z piezo-controlled stage and focus, and two CMOS cameras for dual acquisition. The incubation system encloses the microscope and offers stable environmental conditions with temperature, humidity, and carbon dioxide control for live cell imaging.
|405 nm for PALM||10x/0.3 EC Plan-Neofluar|
|488 nm||20x/0.8 Plan-Apochromat|
|561 nm||40x/1.4 Plan-Apochromat Oil|
|640 nm||63x/1.4 Plan-Apochromat Oil|
|63x/1.46 alpha Plan-Apochromat Oil Corr|
|100x/1.57 Plan-Apochromat Oil|
The Zeiss AxioVert 200M is a motorized, inverted microscope fitted with an incubation system for live cell imaging, providing heating, humidification, and carbon dioxide environmental conditions. The system is equipped with a Hamamatsu ORCA Digital CCD camera, with 70% quantum efficiency and 1344 (H) x 1024 (V) pixels. The scope is capable of phase and DIC microscopy and is fitted with a Lumencor Spectra 7, 6 LED light source for imaging with CFP, GFP, OFP/mCherry, and Cy5. MetaMorph software is used for the acquisition and analysis of data, including the ability to capture long-term, time series with multiple positions.
(When clicked, the video will open a new window. Duration: 43 seconds, Size: 11 MB. )
3 Day experiment, captured on this setup, observing 3T3 cells, in four different positions, was visualized using transmitted light and maintained with 5% carbon dioxide and 37-degree environmental conditions.
Collaborative PALM and diSPIM
Photo-Activated Localization Microscopy (PALM) super-resolution light microscopy is available through collaboration using a home-built, fully automated PALM imaging system in a CCAM associated laboratory. This system is built on ASI’s custom Rapid Automated Modular Mounting (RAMM) platform, uses 440, 515 and 594 nm (Coherent OBIS) laser lines, and is equipped with Hamamatsu sCMOS cameras for image acquisition.
diSPIM (dual-view inverted Selective Plane Illumination Microscope) imaging system from ASI imaging Inc is available through collaboration in a CCAM associated laboratory for lightsheet imaging on the cellular as opposed to tissue scale.