The 880 is mounted on an Axio Observer Z1 with automated xyz control. The system is equipped with a spectral 32-channel QUASAR GaAsp detector allowing for spectral imaging and maximum sensitivity photon counting. There are two adjacent PMT's and a transmitted light detector. The accompanying incubation system, which encloses the microscope, offers stable environmental conditions with temperature, humidity and carbon dioxide control.

 

Lasers	Objectives
405 nm Diode	10x/0.3 EC Plan-Neofluar
458, 488, and 514 nm Argon	20x/0.8 Plan-Apochromat
561 nm  Diode Pumped Solid State (DPSS)	25x/0.8 LD LCI Plan-Apochromat, W,Sil,Gly,Oil
633 nm Helium Neon (HeNe)	40x/1.3 Achrostigmat Oil
	40x/1.2 C-Apochromat Water
	63x/1.4 Plan-Apochromat Oil

The Zeiss LightSheet is an advanced fluorescent microscope that allows you to image optical sections of large, live or fixed samples with virtually no bleaching or phototoxicity to the specimen. The LightSheet also has very fast acquisition speeds, allowing you to capture large amounts of data within a fraction of the time compared to other imaging techniques.

Lasers	Emission Objectives	Excitation Objectives
405 nm Diode	5x/0.16 EC Plan-Neofluar	5x/0.1
488 nm Diode	20x/1.0 Plan-Neofluar Clearing	10x/0.2
561 nm  Diode	20x/1.0 Plan-Apochromat Water	10x/0.2
633 nm Diode

This CCAM user computer is a Windows 7 computer with MetaMorph, Photoshop, Office, Zeiss LSM and Zen Image Browsers.

The 780 is a combined confocal/FCS/NLO system, mounted on an inverted Axio Observer Z1. The 780 employs the spectral 32-channel QUASAR GaAsp detector allowing for spectral imaging, maximum sensitivity photon counting and Fluorescence Correlation Spectroscopy (FCS) as well as two adjacent PMT's. The accompanying incubation system, which encloses the microscope, offers stable environmental conditions with temperature, humidity and carbon dioxide control. The following lasers are on the 780:

• 405 and 440 nm Diode
• 458, 488, and 514 nm Argon
• 561 nm Diode-PUmped Solid State (DPSS)
• 633 nm Helium Neon (HeNe)
• 720 - 950 nm Coherent Chameleon tunable, software controlled, modelocked Ti:Sapphire laser for multi-photon excitation (NLO)

The CC3 is a combined confocal and FCS system, mounted on a AxioVert 200M. This system has two avalanche photodiodes (APD) with ultimate sensitivity allowing for decreased laser power with delicate specimens as well as three traditional PMTs. The APD's are highly efficient in the red wavelength range where fluorescent signal can be very weak.The following lasers are on the Confocor 3:

• 458, 488, and 514 nm Argon
• 561 nm Diode-PUmped Solid State (DPSS)
• 633 nm Helium Neon (HeNe)

The TIRF system is equipped with the Zeiss Axio Observer (inverted) microscope and the Photometric's Evlove 512, a highly sensitive EMCCD camera with 16 bit digitization, and optimum resolution. The fluorescent system captures a thin optical section of approximately 100 -200 nm thick which allows for the visualization of processes taking place near the cell membrane of living cells.This system employs the α-Plan-Fluar 100x/1.45 oil objective. The following lasers are on the TIRF system:

• 488 nm Argon
• 561 nm Diode-PUmped Solid State (DPSS)

Imaris is a 64 bit computer with ImarisSurpass 3-D rendering and analysis software, MetaMorph, Photoshop, and Zeiss Zen Image Browser.

The Spex is a single excitation, dual emission spectrofluorimeter.

Provides tissue culture hood and tissue culture microscope, incubators, refrigeration, and bench space.