Microscopy Equipment

Academic Research Building (ARB)
E6011
263 Farmington Avenue

UConnHealth

Zeiss LSM 880

The 880 is mounted on an Axio Observer Z1 with automated xyz control. The system is equipped with a spectral 32-channel QUASAR GaAsp detector allowing for spectral imaging and maximum sensitivity photon counting. There are two adjacent PMT's and a transmitted light detector. The accompanying incubation system, which encloses the microscope, offers stable environmental conditions with temperature, humidity and carbon dioxide control.

 

Lasers Objectives
 405 nm Diode  10x/0.3 EC Plan-Neofluar
 458, 488, and 514 nm Argon  20x/0.8 Plan-Apochromat
 561 nm  Diode Pumped Solid State (DPSS)  25x/0.8 LD LCI Plan-Apochromat, W,Sil,Gly,Oil
 633 nm Helium Neon (HeNe)  40x/1.3 Achrostigmat Oil
 40x/1.2 C-Apochromat Water
 63x/1.4 Plan-Apochromat Oil

Zeiss LightSheet

The Zeiss LightSheet is an advanced fluorescent microscope that allows you to image optical sections of large, live or fixed samples with virtually no bleaching or phototoxicity to the specimen. The LightSheet also has very fast acquisition speeds, allowing you to capture large amounts of data within a fraction of the time compared to other imaging techniques.

Lasers Emission Objectives Excitation Objectives
 405 nm Diode 5x/0.16 EC Plan-Neofluar 5x/0.1
 488 nm Diode 20x/1.0 Plan-Neofluar Clearing 10x/1.2
 561 nm  Diode 20x/1.0 Plan-Apochromat Water 10x/1.2
 633 nm Diode

Imaging01

This CCAM user computer is a Windows 7 computer with MetaMorph, Photoshop, Office, Zeiss LSM and Zen Image Browsers.

Cell & Genome Sciences Building (CGSB)
Imaging Suite R1510
400 Farmington Avenue

UConnHealth - CGSB

Zeiss 780

The 780 is a combined confocal/FCS/NLO system, mounted on an inverted Axio Observer Z1. The 780 employs the spectral 32-channel QUASAR GaAsp detector allowing for spectral imaging, maximum sensitivity photon counting and Fluorescence Correlation Spectroscopy (FCS) as well as two adjacent PMT's. The accompanying incubation system, which encloses the microscope, offers stable environmental conditions with temperature, humidity and carbon dioxide control. The following lasers are on the 780:

  • 405 and 440 nm Diode
  • 458, 488, and 514 nm Argon
  • 561 nm Diode-PUmped Solid State (DPSS)
  • 633 nm Helium Neon (HeNe)
  • 720 - 950 nm Coherent Chameleon tunable, software controlled, modelocked Ti:Sapphire laser for multi-photon excitation (NLO)

Zeiss Confocor 3

The CC3 is a combined confocal and FCS system, mounted on a AxioVert 200M. This system has two avalanche photodiodes (APD) with ultimate sensitivity allowing for decreased laser power with delicate specimens as well as three traditional PMTs. The APD's are highly efficient in the red wavelength range where fluorescent signal can be very weak.The following lasers are on the Confocor 3:

  • 458, 488, and 514 nm Argon
  • 561 nm Diode-PUmped Solid State (DPSS)
  • 633 nm Helium Neon (HeNe)

Zeiss TIRF

The TIRF system is equipped with the Zeiss Axio Observer (inverted) )microscope and the Zeiss AxioCam MRm, a 12 bit, high sensitivity, optimum resolution camera. The fluorescent system captures a thin optical section of approximately 100 -200 nm thick which allows for the visualization of processes taking place near the cell membrane of living cells.This system employs the α-Plan-Fluar 100x/1.45 oil objective. The following lasers are on the TIRF system:

  • 488 nm Argon
  • 561 nm Diode-PUmped Solid State (DPSS)

Imaris

Imaris is a 64 bit computer with ImarisSurpass 3-D rendering and analysis software, MetaMorph, Photoshop, and Zeiss LSM and Zen Image Browsers.

CCAMTerm

This CCAM user computer is a PC equipped with MetaMorph, Photoshop, ImageJ, Zeiss LSM and Zen Image Browsers and a Flatbed Scanner.

Spex

The Spex is a single excitation, dual emission spectrofluorimeter.

CCAM Wet Lab

Provides tissue culture hood and tissue culture microscope, incubators, refrigeration, and bench space.