The 880 is mounted on an Axio Observer Z1 with automated xyz control. The system is equipped with a spectral 32-channel QUASAR GaAsp detector allowing for spectral imaging and maximum sensitivity photon counting. This detector is effective from 410 - 696 nm. There are two adjacent PMT's, effective range 415 - 735 nm, and a transmitted light detector. The accompanying incubation system, which encloses the microscope, offers stable environmental conditions with temperature, humidity and carbon dioxide control.

Lasers	Objectives
405 nm Diode	10x/0.3 EC Plan-Neofluar
458, 488, and 514 nm Argon	20x/0.8 Plan-Apochromat
561 nm  Diode Pumped Solid State (DPSS)	25x/0.8 LD LCI Plan-Apochromat, W,Sil,Gly,Oil
633 nm Helium Neon (HeNe)	40x/1.3 Achrostigmat Oil
	40x/1.2 C-Apochromat Water
	63x/1.4 Plan-Apochromat Oil

The Zeiss LightSheet is an advanced fluorescent microscope that allows you to image optical sections of large, live or fixed samples with virtually no bleaching or phototoxicity to the specimen.  The optics are equipped for water-based or cleared specimens and dual sCMOS cooled cameras to provide high sensitivity, rapid, 3D imaging of live or fixed thick specimens. The extremely fast acquisition speeds allow you to capture large amounts of data within a fraction of the time compared to other imaging techniques.

Lasers	Emission Objectives	Excitation Objectives
405 nm Diode	5x/0.16 EC Plan-Neofluar	5x/0.1
488 nm Diode	20x/1.0 Plan-Neofluar Clearing	10x/0.2
561 nm  Diode	20x/1.0 Plan-Apochromat Water	10x/0.2
633 nm Diode

This CCAM user computer is a Windows 7 computer with MetaMorph, Photoshop, Office, Zeiss LSM and Zen Image Browsers. All the software is available, with prior approval, via remote access.

The 780 is a combined confocal/FCS/NLO system, mounted on an inverted Axio Observer Z1. The 780 employs the spectral 32-channel QUASAR GaAsp detector allowing for spectral imaging, maximum sensitivity photon counting and Fluorescence Correlation Spectroscopy (FCS) as well as two adjacent PMT's.  The GaAsp detector has a detection range of 410 - 696 nm while the PMTs have a range from 415 - 735 nm. The accompanying incubation system, which encloses the microscope, offers stable environmental conditions with temperature, humidity and carbon dioxide control. This microscope is also equipped with a Becker-Hinkl Fluorescence Lifetime Imaging Microscopy (FLIM) detector on a nondescanned detection port, particularly useful for detection of fluorescence resonance energy transfer (FRET) based probes.

Lasers	Objectives
405 nm and 440 nm Diode	10x/0.45 W C-Apochromat
458, 488, and 514 nm Argon	20x/0.8 Plan-Apochromat
561 nm  Diode Pumped Solid State (DPSS)	40x/1.2 W C-Apochromat
633 nm Helium Neon (HeNe)	40x/1.3 Oil Plan-Neofluar
720 - 950 nm Coherent Chameleon tunable, software controlled, modelocked Ti:Sapphire laser for multi-photon excitation (NLO)	63x/1.4 Plan-Apochromat Oil

The CC3 is a combined confocal and FCS system, mounted on a AxioVert 200M. This system has two avalanche photodiodes (APD) with ultimate sensitivity allowing for decreased laser power with delicate specimens as well as three traditional PMTs. The APD's are highly efficient in the red wavelength range where fluorescent signal can be very weak.
 
 
 
The following lasers are on the Confocor 3:

• 458, 488, and 514 nm Argon
• 561 nm Diode-PUmped Solid State (DPSS)
• 633 nm Helium Neon (HeNe)

Photo-Activated Localization Microscopy (PALM) super-resolution light microscopy is available through collaboration using a home-built, fully automated PALM imaging system in a CCAM associated laboratory. This system is built on ASI’s custom Rapid Automated Modular Mounting (RAMM) platform, uses 440, 515 and 594 nm (Coherent OBIS) laser lines, and is equipped with Hamamatsu sCMOS cameras for image acquisition.
 
 
diSPIM (dual-view inverted Selective Plane Illumination Microscope) imaging system from ASI imaging Inc is available through collaboration in a CCAM associated laboratory for lightsheet imaging on the cellular as opposed to tissue scale.

Imaris is a 64 bit computer with ImarisSurpass 3-D rendering and analysis software, MetaMorph, Photoshop, and Zeiss Zen Image Browser.

The Spex is a single excitation, dual emission spectrofluorimeter.

Provides tissue culture hood and tissue culture microscope, incubators, refrigeration, and bench space.