Imaging Tips for the Zeiss Zen 780 & 880

Auto Focus

Often when conducting long-term, live experiments, your cells will drift out of focus. To avoid this situation, the Zeiss 780 and 880 confocals have an auto-focus routine that can correct for the drift over the length of your experiment. This can be used for single or multiple positions, and with dry or immersion objectives.



Bleed-through can occur when fluorescence from one probe overlaps the emission of another probe. In this video, we demonstrate the concept of bleed-through, and then discuss two ways of avoiding this situation when using the Zeiss 780 or Zeiss 880. (Closed Caption Version)