Auto Focus

Often when conducting long-term, live experiments, your cells will drift out of focus. To avoid this situation, the Zeiss 780 and 880 confocals have an auto-focus routine that can correct for the drift over the length of your experiment. This can be used for single or multiple positions, and with dry or immersion objectives.

 

Bleed-Through

Bleed-through can occur when fluorescence from one probe overlaps the emission of another probe. In this video, we demonstrate the concept of bleed-through, and then discuss two ways of avoiding this situation when using the Zeiss 780 or Zeiss 880. (Closed Caption Version)

 

Setting the Pinhole

You know the pinhole improves resolution on the Zeiss 780 and 880 confocals, but how do you set it? This video will review the purpose of the pinhole, demonstrate how it improves your images and then explain how to correctly set the pinhole when you are imaging with single and multiple probes. (Closed Caption Version)