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Super Resolution

Zoomed wide field fluorescent microscopy image of S. aureus stained with Nile Red. (J.Batchelder, Mok Lab)
Zoomed SIM image of S. aureus stained with Nile Red. (J.Batchelder, Mok Lab)
Widefield microscope image of mitochondria. (CCAM)
SIM image of mitochondria. (CCAM)
Widefield microscope 3D stack of mitochondria. (CCAM)
SIM 3D stack of mitochondria. (CCAM)
Zoomed Widefield Image of MAP2 Stained Neuronal cultures. (K. Caro, Martinelli Lab)
STORM Image of MAP2 Stained Dendrites. (K. Caro, Martinelli Lab)

Super Resolution Techniques

Technique	System	Lateral Resolution	Axial Resolution	Fluorophores
SIM	Widefield	~120 nm	~ 250 nm	Standard
SMLM	Widefield	~20 nm	~20 nm	Photoswitchable, Photoactivatable
STED	Confocal	~20 nm	~50 nm	Standard

SIM - Structured illumination microscopy
SMLM - Single-molecule localization microscopy:

PALM - Photoactivated localization microscopy
STORM - Stochastic optical reconstruction microscopy
PAINT - Point accumulation in nanoscale topography

STED - Stimulated emission depletion