The CCAM Microscopy Facility provides the UConn Health research community as well as other academic and industrial institutes, access to its state-of-the-art equipment for quantitative fluorescence imaging applications. Within the facility, there are two laser scanning confocal microscopes equipped with 34-channel high-efficiency QUASAR detectors. One of these microscopes supports nonlinear optical (also known as 2-photon) excitation, fluorescence correlation spectroscopy (FCS), and fluorescence lifetime imaging microscopy (FLIM). Additionally, there is a light sheet microscope available for micro/mesoscale 3D imaging. Furthermore, the facility recently acquired a state-of-the-art Zeiss Elyra 7 microscope that is capable of total internal reflection fluorescence microscopy (TIRFM) and supports several super-resolution techniques such as lattice structured illumination microscopy (SIM), stochastic optical reconstruction microscopy (STORM), 3D photoactivated localization microscopy (PALM), and DNA points accumulation for imaging in nanoscale topography (DNA-PAINT).
The equipment may be used independently by authorized users who have completed the required training for each piece of equipment they choose to use. Alternatively, CCAM staff are available for assisted use. CCAM offers data storage space to each lab with an active account. Please contact Dr. Yi Wu, yiwu@uchc.edu, or Susan Staurovsky, staurovsky@uchc.edu, for additional information or assistance.
![SIM of MAP2 Neuronal Culture](https://health.uconn.edu/cell-analysis-modeling/wp-content/uploads/sites/149/2023/08/SIM-MAP2-Neuronal-Culture-adj2.png)
SIM Image of Primary Neuronal Culture, K. Caro.
![LightSheet Midgut Drosophila Melanogaster cmyk colors](https://health.uconn.edu/cell-analysis-modeling/wp-content/uploads/sites/149/2021/02/LightSheet-Kavitha-cmyk-colors-2.png)
LightSheet Midgut Drosophila Melanogaster, K. Kannan.
![SIM Image of Mitochondria](https://health.uconn.edu/cell-analysis-modeling/wp-content/uploads/sites/149/2023/08/MT-SIM-Single-Plane-Color.png)
SIM Image of Mitochondria, Y. Wu.
Equipment
Fees
Microscope Access
For your convenience, please use the following document for a detailed listing of the equipment and software resources available at the CCAM Microscopy Facility.
![Blocks with Grant Support](https://health.uconn.edu/cell-analysis-modeling/wp-content/uploads/sites/149/2021/06/grant-support.png)