Creating a Large Image

When you have a large sample that requires you to take multiple images and align them together, you can use the Large Image application on the Nikon Spinning Disk. This function captures a series of images and stitches them together in a tile like fashion displaying the image in its entirety.

 

Using the Specimen Navigator

The specimen navigator is a useful utility for creating a low resolution preview of your sample. You can then use this image to navigate to various regions of your sample for observation under a higher resolution objective.

 

Acquiring a Z-Stack using Nikon’s Perfect Focus System

When capturing 3D data sets, you can us the Perfect Focus System (PFS) while using the asymmetric means of collecting a z-stack. Typically you do not use the PFS while collecting a z-stack, but you can do so while using the asymmetric option. This is extremely helpful when collecting data from a number of different locations on your sample.