Introduction to the PFS – Perfect Focus System

Did you ever have trouble with focus drift when doing your live cell imaging? If you can relate, then sit back and enjoy this short video explaining short the PFS and the benefits for using the system. (Closed Caption Version )

 

Creating a Large Image

When you have a large sample that requires you to take multiple images and align them together, you can use the Large Image application on the Nikon Spinning Disk. This function captures a series of images and stitches them together in a tile like fashion displaying the image in its entirety.

 

Using the Specimen Navigator

The specimen navigator is a useful utility for creating a low resolution preview of your sample. You can then use this image to navigate to various regions of your sample for observation under a higher resolution objective.

 

Acquiring a Z-Stack using Nikon’s Perfect Focus System

When capturing 3D data sets, you can us the Perfect Focus System (PFS) while using the asymmetric means of collecting a z-stack. Typically you do not use the PFS while collecting a z-stack, but you can do so while using the asymmetric option. This is extremely helpful when collecting data from a number of different locations on your sample.