Assistant Professor (In Residence)
Assistant Professor (In Residence)
As a postdoctoral fellow in the Jaffe lab, I’m participating in a project regarding the signaling mechanisms by which luteinizing hormone causes meiotic resumption in the ovarian follicle. Prior to joining this lab, I did my Master of Science and Ph.D. research in the laboratory of Developmental Biology at the University of Pavia (Italy), with Dr. Garagna and Dr. Zuccotti, where I specialized on the biology of the ovarian follicle. My research projects were mainly focused on the identification of murine follicle cell markers of the oocyte developmental competence. I’m now interested in more functional aspects of the female gamete growth and maturation.
My work has been mainly focused on the study of cAMP-hydrolyzing phosphodiesterases in granulosa cells of the preovulatory ovarian follicle. Our recent study has shown that multiple cAMP phosphodiesterases are involved in the control of cAMP levels prior to the LH surge. By suppressing spontaneous and premature cAMP elevations, these phosphodiesterases act together to prevent premature progression of meiosis and ovulation. This research contributes to our understanding of the mechanisms underlying the maintenance of meiotic arrest prior to the LH surge.
Giulia Vigone, Leia C. Shuhaibar, Jeremy R. Egbert, Tracy F. Uliasz, Matthew A. Movsesian, and Laurinda A. Jaffe. (2018) Multiple cAMP phosphodiesterases act together to prevent premature oocyte meiosis and ovulation. Endocrinology. 159: 2142-2152.
Poster About Current Research
Vigone, G, Egbert, JR., and Jaffe, LA. Synergistic activities of multiple cyclic AMP phosphodiesterases prevent premature meiotic progression, ovulation and progesterone signaling in mouse ovarian follicles. Gordon Research Seminar and Conference “Fertilization and Activation of Development”, Holderness School, Holderness, NH, USA – July 15-21, 2017.
Ph.D. Biomedical Science Student
I am a graduate student in Cell Biology working in Dr. Jaffe’s lab. I previously completed my M.S. in Agriculture working with Dr. Fernando Campos-Chillon on the metabolism and cryopreservation of in vitro produced cattle embryos. During my Master’s, I became interested in the oocyte and how meiotic arrest is maintained within the ovarian follicle. Now, I study the luteinizing hormone receptor expression and the signaling pathways that are activated when luteinizing hormone binds to its receptor.
Research Assistant 3
I am a research assistant in the Department of Cell Biology working jointly in the labs of Dr. Laurinda Jaffe and Dr. Lisa Mehlmann. Both labs are interested in the signaling pathways that regulate meiotic cell cycling. In the Jaffe lab, I have been involved in the study of the regulation and activity of guanylyl cyclase (NPR2) and its ligand CNP, which function to maintain high levels of cGMP in the ovarian follicle and meiotic arrest of the oocyte. We are investigating how luteinizing hormone regulates this system to cause meiosis to resume. In the Mehlmann lab the work I am involved in is focused on how the signaling pathway of the constitutively active GPR3 receptor regulates cAMP levels which is also involved in the maintenance of meiotic arrest.
Research Assistant 1
I began working as a research assistant in Dr. Jaffe’s lab this past summer (in July 2019). Since then, I’ve been learning and practicing many new techniques from operating a confocal microscope to genotyping mice to presenting statistical analyses using GraphPad Prism. I received my bachelor’s degree from Trinity College where I majored in Biology and Hispanic Studies. I also spent two summers working in Dr. Daniel Blackburn’s lab studying lizard, turtle, and alligator eggs. Using a scanning electron microscope and light microscopy techniques, we wanted to know how these organisms organize a network of blood vessels to absorb nutrients from the yolk. I look forward to continue doing research here at UConn Health!
Past Lab Members
- Seth Lapuk (Postbac, 1982-1983), Pediatric Cardiologist, Connecticut Children’s Medical Center, Hartford, CT, and Associate Clinical Professor of Pediatrics UConn School of Medicine
- Ana Iglesias (Postbac, 1983-1984), Professor, Universidad Politechnica de Madrid, Spain
- Lyanne Schlichter (Postdoc, 1983-1984), Professor, University of Toronto, Canada
- Lavinia (Muncy) Ross (Research Assistant, 1984-1990, 2001-2003), Musician, Sweet Home, OR
- Doug Kline (Postdoc, 1985-1988), Emeritus Professor, Kent State University
- Paul Turner (Ph.D., 1987), Field Applications Specialist, Affymetrics
- Kazuyoshi Chiba (Postdoc, 1988-1989), Professor, Ochanomizu University, Tokyo, Japan
- Fraser Shilling (Postdoc, 1991-1993), Co-Director, Road Ecology Center, University of California, Davis
- Chris Gallo (Postdoc, 1991-1995), Director, BioProcess Research and Development, Pfizer, Andover, MA
- David Carroll (Ph.D., 1996), Professor, Florida Institute of Technology
- David Serwanski (Research Assistant, 1995-2002), Retired
- Linda Runft (Ph.D., 2000), Medical Writing Senior Manager, Amgen Inc.
- Lisa Mehlmann (Postdoc, 1996-2001), Associate Professor, UConn Health Center, CT
- Andrew Giusti (Postdoc, 2000-2002), Deceased
- Rebecca Kalinowski (Ph.D., 2004), Director at Expense Reduction Analysts, Hartford. Specializing in Medical, Pharmaceutical, and Laboratory Cost Reduction
- Leon Freudzon (Postbac, 2004-2005), Assistant Professor and Associate Fellowship Program Director, Anesthesiology, Yale School of Medicine.
- Marina Freudzon (Undergraduate Honors Student and Postbac, 2005-2007), Dermatologist, Kaiser Permanente, Atlanta GA
- Mikhail Tsesis (Postbac, 2009-10), Dentist, Putnam, CT
- Rachael Norris (Ph.D., 2009), Postdoc, UConn Health Center, CT
- William Ratzan (Ph.D., 2011), Postdoc, Montana State University, MT
- Valentina Baena (Postbac, 2013-2015), Graduate Student, UConn Health Center