Design and Generation of Targeting Constructs Using Recombineering-based Method

We design and generate gene targeting constructs. The constructs generated in the Center for Mouse Genome Modification (CMGM) include conventional knock-out, knock-in, conditional knock-out, knock-in subtle mutations such as a point mutation and small deletion, and other custom made constructs.

The constructs are derived from C57Bl/6 genomic DNA, unless investigator provides the BAC clones from other strains. We use C57Bl/6 x 129SVEV F1 hybrid ES cell lines for targeting.

Investigators who plan to make a transgenic/targeting construct please contact the CMGM at

We will need the construct purpose- conventional or conditional knock-out, point mutation or deletion as well as the MGI number or Genbank accession number.

The service fees cover the materials and technician time. It does not cover the construct design, trouble shooting and consultation during generation of targeting animals. Thus the project should be considered as a collaboration.