Basic hPSC culture techniques
The hPSC model, with the advent of reprogramming and gene editing, presents an opportunity for incredible access and insight to human development, health, and disease. The UConn Stem Cell Core provides training for UConn and UConn Health Researchers pursuing hPSC work. This training provides an introduction and overview to the technical process of obtaining and initiating hPSC culture.
Lab activities cover the basics, including preparation of culture medium, identification of undifferentiated hPSCs and non-specific differentiation, methods of selection of undifferentiated hPSCs, and spliting/subcloning. Participants get the opportunity to view and handle hPSCs in the lab. Discussion on culturing hPSC on feeder independent platforms, such as mTeSR and matrigel, or other topic suggestions are welcome. Training is tailored to your needs.
This is basic hPSC tissue culture methods training instruction.
As a member of UConn Health or UConn, the stem cell core is available to you for as a support resource for human stem cell culture applications. We offer a reprogramming service, gene editing, karyotyping, and sequencing services, and have extensive expertise in neural differentiation. The UConn Stem Cell Core has trained over 200 staff laboratory scientists, students, post doc fellows, faculty members, and research assistants since 2007.
Novel and state-of-the-art technologies are being developed dynamically on basic and translational research in the areas of pluripotent stem cells, early human development, stem cell culture differentiation, epigenetics, and stem cell gene editing technologies. To catch up on these themes and others, we host workshops by inviting experts and pioneers.
Visit the Institute of Systems Genomics for a workshop listings.