Basic hPSC culture techniques
The hPSC model for human development, health, and disease research is a great investment. With the advent of reprogramming and gene editing, the technology is well worth the effort. First you need a foundation. Unfamiliarity can make the process seem overwhelming. The UConn Stem Cell Core provides training for UConn Health or UConn members who are beginning hPSC work, or who desire a refresher. This training provides and introduction and overview – it can demystify the process, while beginning to prepare you for some lengthy, but rewarding work.
Lab activities cover the basics, including preparation of culture medium, identification of undifferentiated hPSCs and non-specific differentiation, methods of selection of undifferentiated hPSCs, and spliting/subcloning. Participants get the opportunity to view and handle hPSCs in our lab. Discussion on culturing hPSC on feeder independent platforms, such as mTeSR and matrigel, or other topic suggestions are welcome. Training is tailored to your needs.
This is basic hPSC tissue culture methods training instruction.
As a member of UConn Health or UConn, the stem cell core is available to you for advice about human stem cell culture, including reprogramming. We offer a reprogramming service, and our close colleagues offer gene editing, karyotyping, and sequencing services, and have extensive expertise in neural differentiation. The UConn Stem Cell Core has trained over 150 staff members, students, post doc fellows and other laboratory scientists since 2007.
Novel and state-of-the-art technologies are being developed dynamically on basic and translational research in the areas of pluripotent stem cells, early human development, stem cell culture differentiation, epigenetics, and stem cell gene editing technologies. To catch up on these themes and others, we host workshops by inviting experts and pioneers.
Visit the Institute of Systems Genomics for a workshop listings.