Validation and Quality Control Analysis
Verification of Pluripotency
iPSCs are differentiated into embryoid bodies and specification of endoderm, ectoderm, and mesoderm lineages are assayed by qRT-PCR using the Applied Biosystems® TaqMan® hPSC Scorecard.
We provide a biochemical mycoplasma test using the Lonza Myco-Alert kit®. We ask that you submit 1 – 2 ml of spent culture medium to be tested.
Please see the Services Pricing list for current prices. For more information on these services, please contact us.
Genome Integrity Analysis
Genetics and Genomics Division of the iPS Cell and Chromosome Core
The Genetics and Genomics Division of the Core was established as the first cross-campus core facility. Studies have reported that many different cell lines, including human pluripotent stem cells (including human ES and iPS cells), are prone to chromosome abnormalities that compromise their integrity and therapeutic potential. Likewise, cell lines produced to mimic human disease or a specific patient genotype (e.g. autism and Prader Willi syndrome) require confirmation of specific gene deletions, duplications, insertions, etc. We provide a full suite of services to characterize the integrity of your cell lines for your scientific research.
- Karyotype analysis of G-banded metaphase chromosomes
- Fluorescence in situ hybridization (FISH)
- SNP and CNV microarray analysis
- Lineage-specific gene expression profiling
We will perform QC measures, expand, store, and distribute your custom hESC and iPSC lines when requested by your collaborators. We can also provide continuous banking as a back-up repository for your lab.
For more information on this service, please contact us.
We provide training in iPSC/hESC Basic Culture Techniques, consulting, and troubleshooting support. Individual workshops are available for iPSC/hESC differentiation to neural, cardiomyocyte, and vascular endothelial lineages.
Since training sessions are customized to each user’s skill level, training is charged by the hour.