Stem Cell Quality Control Services and Reagents

Once hiPSC or hESC lines have been generated, what validation is needed? How should quality control be established and maintained?

1. Establish hPSC line – Generate hiPSC or hESC

2. For all cell cultures, implement a regular mycoplasma testing schedule.

To submit samples:

  • Please fill out the mycoplasma testing submission form.
  • Write the name of the samples clearly on your tube(s). Your sample names written on the form should match what is written on your tube(s).
  • Submit the order through the UConn Stem Cell Core CORES System.
  • When you are ready to submit your samples, contact us to set up details for drop off.

Details about sample preparation:

  • Two mls of cell culture supernatant should be submitted to the stem cell core.  Cell culture supernatant for this assay can be stored 2°C-8°C for 1 week or -20°C for up to 2 months.
  • Please submit culture medium from cells that have been grown without antibiotics such as Pen-Strep for at least 1 week.
  • You may see the attached manufacturer’s FAQ for the Mycoalert kit, which addresses additional questions about how to prepare samples for this assay.

3. Validate Morphology

4. Ability to attach and proliferate (cloning efficiency) – when passaged or thawed

5. Cryopreservation, including test thaw validation

6. Scale Up and Cell Bank – Good cell banking practices

7. Identity – DNA profile

8. Assess pluipotency and self-renewal/stemness

There are a variety of options to demonstrate pluripotency.  Some options are listed below:

9. Evaluate and monitor Genetic Stability

10. Epigenetic Stability

11. Monitor and re-test hPSC cultures as needed, including:

12. More references
(*) see these references for more overviews of hPSC characterization: