Instrumentation

Becton-Dickinson FACSCalibur

Becton-Dickinson FACSCalibur

There are two caliburs, one in E6014 and one in L3051. These are capable of analyzing cells stained with up to four different fluorescent markers (typically FITC, PE, PerCP, and APC).

Becton-Dickinson LSR II

Becton-Dickinson LSR II

There are three LSR II analyzers. Two (LSR II-A and LSR II-C) have four lasers and one (LSR II-B) has five. In their current configuration these analyzers are able to detect 15 to 18 parameters (13 to 15 colors plus forward and side scatter). LSR II-B and LSR II-C are considered special order research products (SORP) and are customized for ongoing research at UConn Health.

Becton-Dickinson FacsAria II

Becton-Dickinson FacsAria II

Our high-speed cell sorters each have five lasers. Aria II-A can detect up to 15 parameters (13 colors plus forward and side scatter). Aria II-B can detect up to 18 parameters (16 colors plus forward and side scatter). Both Aria II cell sorters are equipped with an aerosol containment unit to allow sorting of biohazardous materials. Additionally, both Aria II cell sorters are equipped with an automated cell deposition unit (ACDU) that allows for sorting into plates or glass slides. The Aria II sorters are considered special order research products (SORP) and are customized for ongoing research at UConn Health.