C1ql3-Expressing Neuronal Circuit Analysis

C1ql3-Expressing Neuronal Circuit Analysis

We discovered that C1ql3-expressing neurons in the basolateral amygdala (BLA) send axonal projections to the medial prefrontal cortex (mPFC). A rabies virus (which infects neurons retrogradely) was injected into the mPFC. This infected the pre-synaptic terminals of the BLA neurons, resulting in the expression of the red colored tdTomato protein in the soma of infected neurons. Co-fluorescence of tdTomato and the green colored mVenus protein which marks C1ql3-expressing neurons can be seen in the BLA, in panels C-E