| ADDRESS: | Department of Cell Biology |
| University of Connecticut Health Center | |
| Farmington, CT 06030 USA | |
| Phone: (860) 679-2661 | |
| e-mail: ljaffe@uchc.edu | |
| BIRTH: | January 9, 1952, Pasadena, CA |
EDUCATION:
| University of Wisconsin, Madison, WI | 1969-1971 |
| Purdue University, W. Lafayette, IN
B.S. (biology) |
1971-1973 |
| University of California, Los Angeles, CA
Ph.D. (biology; advisor: Susumu Hagiwara) |
1974-1977 |
| Marine Biological Laboratory, Woods Hole, MA
post-doc (advisor: Lewis Tilney) |
1978-1979 |
| University of California, San Diego, CA
post-doc (advisor: Meredith Gould) |
1979-1981 |
FACULTY POSITIONS:
University of Connecticut Health Center, Farmington, CT
| Assistant Professor – Professor | 1981-present |
| Interim Chair | 2006-2011 |
| Chair | 2011-present |
AWARDS:
UConn Health Loeser Award for Outstanding Teaching, 1988
National Science Foundation Faculty Award for Women, 1991-1997
Human Frontier Science Program Award, 2000-2004
Fellow, American Association for the Advancement of Science, 2007
MERIT Award, NICHD, 2014-2024
Frontiers in Reproduction Pioneer Award, 2017
UConn Health Dean’s Award for Excellence in Research Mentoring, 2018
RECENT OTHER PROFESSIONAL ACTIVITIES:
| 1983 – present | Faculty for summer courses at the Marine Biological Lab (Woods Hole)
(Embryology, Frontiers in Reproduction) |
| 1986 – 2007 | Associate editor, Developmental Biology |
| 2007 – present | Editorial board, Developmental Biology |
| 1998 – 2012 | NIH study sections (member: Cell Development and Function, 1998-2002; Cellular, Molecular, and Integrative Reproduction, 2006-2012) |
| 2011- 2016 | NICHD Board of scientific counselors |
| 2000 – present | MBL Committee on Courses (Woods Hole) (chair since 2012) |
| 2012 | Co-organizer, 2012 Cold Spring Harbor meeting on Germ Cells |
| 2017 – 2019 | Co-organizer, 5th International Oocyte Meeting |
| 2017 – 2022 | Scientific Advisory Board, Max Planck Institute for Biophysical Chemistry |
| 2018 – 2021 | Scientific Advisory Council, Frontiers in Reproduction Program (Burroughs Welcome Fund) |
| 2019 – 2024 | Scientific Advisory Board, Villefranche-sur-Mer Developmental Biology Laboratory (Sorbonne Université – CNRS) |
INVITED PRESENTATIONS (2005-2019):
| March, 2005 | Seminar at University of Connecticut, Storrs |
| March, 2005 | European Molecular Biology Organization, conference on “Meiotic Divisions and Checkpoints” (Corsica, France) |
| July, 2005 | NICHD workshop on”Gamete Interaction” |
| July, 2005 | Gordon Conference on “Fertilization and the Activation of Development” |
| November, 2005 | Seminar at Boston College |
| April, 2006 | Seminar at University of Pennsylvania |
| May, 2006 | Seminar at Northwestern University |
| June, 2006 | Gordon Conference on “Mammalian Gametogenesis and Embryogenesis” |
| June, 2006 | Endocrine Society Meeting, symposium talk |
| November, 2006 | Symposium on “Cell Signaling in Gamete Activation: from Basic Research to ART” (Tokyo, Japan) |
| November, 2006 | Seminar at Tokyo Institute of Technology |
| February, 2007 | Seminar at Florida Institute of Technology |
| February, 2007 | Seminar at University of Florida, Whitney Lab |
| July, 2007 | Seminar at Marine Biological Lab, Woods Hole |
| March, 2008 | Seminar at the Fred Hutchinson Cancer Research Center, Seattle WA |
| October, 2008 | Seminar at the Vollum Institute, Portland OR |
| April, 2009 | Seminar at Ottawa Health Research Institute, Ottawa, Canada |
| June, 2009 | Conference on “Meiotic Divisions in Oocytes” (Tourtour, France) |
| June, 2009 | Seminar at the European Molecular Biology Laboratory, Heidelberg, Germany |
| July, 2009 | Seminar at University of Wuerzburg, Germany |
| July, 2009 | Gordon Conference on “Cell Fusion” |
| November, 2009 | Keynote speaker for “Réseau Québécois en Réproduction” Symposium, Montreal |
| March, 2010 | Seminar at University of Pennsylvania |
| June, 2010 | Jean Clark Dan Symposium, Woods Hole |
| September, 2010 | Keynote speaker for meeting on “Mechanisms of Egg Maturation and Fertilization”, University of Washington, Friday Harbor Labs |
| October, 2010 | Cold Spring Harbor meeting on Germ Cells |
| December, 2010 | Seminar at University of Massachusetts, Amherst |
| March, 2011 | Seminar at University of Massachusetts, Worcester |
| June, 2011 | Seminar at University of Minnesota, Minneapolis |
| December, 2011 | Speaker at meeting of American Society for Cell Biology |
| April 2012 | Seminar at National Institute of Environmental Health Sciences, North Carolina |
| June 2012 | Speaker at NICHD Intramural Scientific Retreat, Bethesda |
| February, 2013 | Seminar at Washington State University |
| April, 2013 | Seminar at Cornell University |
| June, 2013 | Speaker at EMBO conference on “Oocyte Maturation and Fertilization” |
| June, 2013 | Seminar at University of Giessen, Germany |
| November, 2013 | Seminar at Connecticut Children’s Hospital |
| March, 2014 | Seminar at University of Connecticut, Storrs |
| March, 2015 | Seminar at University of Washington, Seattle |
| June, 2015 | Speaker at conference on “Oocyte Maturation and Fertilization”, Japan |
| February, 2016 | Seminar at University of Pittsburgh |
| November, 2016 | Seminar at University of Minnesota |
| June, 2017 | Keynote speaker for Frontiers in Reproduction Symposium, Woods Hole |
| June, 2017 | 8th International Conference on cGMP, Germany |
| July, 2017 | Gordon Conference on “Fertilization and the Activation of Development” |
| April, 2018 | Seminar at the European Molecular Biology Laboratory, Heidelberg, Germany |
| April, 2018 | 117th International Titisee Conference, From Oocyte to Embryo, Germany |
| April, 2018 | Seminar at University of Hamburg, Germany |
| July, 2018 | Speaker at meeting of the Society for the Study of Reproduction |
| January, 2019 | Seminar at Imagine Institute, Paris, France |
RECENT FUNDING:
| 1981- 2024 | NIH, R01/R37-HD14939, “Signal Transduction at Fertilization” |
| 2000-2004 | Human Frontiers Science Program, “Signal Transduction during Oocyte Maturation” |
| 2005-2010 | NIH, R01-DK073499, “Regulation of meiotic arrest by a Gs-linked receptor” |
| 2010- 2013 | NIH, R21HD067017, “Function of a voltage-sensitive phosphatase in the regulation of sperm-egg fusion” |
PUBLICATIONS:
Jaffe, L.A., Weisenseel, M.H. and Jaffe, L.F. (1975). Calcium accumulations within the growing tips of pollen tubes. J. Cell Biol. 67: 488-492.
Jaffe, L.A. (1976). Fast block to polyspermy in sea urchin eggs is electrically mediated. Nature 261:68‑71.
Jaffe, L.A. and Robinson, K.R. (1978). Membrane potential of the unfertilized sea urchin egg. Develop. Biol. 62: 215-228.
Jaffe, L.A., Hagiwara, S. and Kado, R.T. (1978). The time course of cortical vesicle fusion in sea urchin eggs observed as membrane capacitance changes. Develop. Biol. 67: 243-248.
Jaffe, L.A., Gould-Somero, M. and Holland, L. (1979). Ionic mechanism of the fertilization potential of the marine worm, Urechis caupo (Echiura). J. Gen. Physiol. 73: 469-492.
Gould-Somero, M., Jaffe, L.A. and Holland, L.Z. (1979). Electrically-mediated fast polyspermy block in eggs of the marine worm, Urechis caupo. J. Cell Biol. 82: 426-440.
Hagiwara, S. and Jaffe, L.A. (1979). Electrical properties of egg cell membranes. Ann. Rev. Biophys. Bioeng. 8: 385-416.
Jaffe, L.A. (1980). Electrical polyspermy block in sea urchins. Nicotine and low sodium experiments. Develop. Growth. Differ. 22: 503-507.
Tilney, L.G. and Jaffe, L.A. (1980). Actin, microvilli, and the fertilization cone of sea urchin eggs. J. Cell Biol. 87: 771-782.
Jaffe, L.A. and Guerrier, P. (1981). Localization of electrical excitability in the early embryo of Dentalium. Develop. Biol. 83: 370-373.
Jaffe, L.A., Gould-Somero, M. and Holland, L.Z. (1982). Studies of the mechanism of the electrical polyspermy block using voltage clamp during cross-species fertilization. J. Cell Biol. 92: 616-621.
Jaffe, L.A., Sharp, A.P. and Wolf, D.W. (1983). Absence of an electrical polyspermy block in the mouse. Develop. Biol. 96: 317-323.
Jaffe, L.A. (1983). Fertilization potentials from eggs of the marine worms Chaetopterus and Saccoglossus. In The Physiology of Excitable Cells, eds. W.J. Moody and A.D. Grinnell. Alan R. Liss, New York, pp. 211-218.
Jaffe, L.A., Cross, N.L. and Picheral, B. (1983). Studies of the voltage-dependent polyspermy block using cross-species fertilization of amphibians. Develop. Biol. 98: 319-326.
Jaffe, L.A. and Cross, N.L. (1984). Electrical properties of vertebrate oocyte membranes. Biol. Reprod. 30: 50-54.
Gould-Somero, M. and Jaffe, L.A. (1984). Control of cell fusion at fertilization by membrane potential. In Cell Fusion: Gene Transfer and Transformation, eds. R.F. Beers, Jr. and E.G. Bassett. Raven Press, New York, pp. 27-38.
Turner, P.R., Sheetz, M.P. and Jaffe, L.A. (1984). Fertilization increases the polyphosphoinositide content of sea urchin eggs. Nature 310: 414-415.
Jaffe, L.A. and Schlichter, L.C. (1985). Fertilization-induced ionic conductances in eggs of the frog, Rana pipiens. J. Physiol. 358: 299‑319.
Jaffe, L.A., Kado, R.T. and Muncy, L. (1985). Propagating potassium and chloride conductances during activation and fertilization of the egg of the frog, Rana pipiens. J. Physiol. 368: 227-242.
Jaffe, L.A. and Gould, M. (1985). Polyspermy prevention mechanisms. In Biology of Fertilization, vol. 3, ed. C.B. Metz and A. Monroy. Academic Press, New York, pp. 223‑250.
Kline, D., Jaffe, L.A. and Tucker, R.P. (1985). Fertilization potential and polyspermy prevention in the egg of the nemertean, Cerebratulus lacteus. J. Exp. Zool. 236: 45-52.
Turner, P.R., Jaffe, L.A. and Fein, A. (1986). Regulation of cortical vesicle exocytosis in sea urchin eggs by inositol 1,4,5-trisphosphate and GTP-binding protein. J. Cell Biol. 102: 70-76.
Jaffe, L.A. and Cross, N.L. (1986). Electrical regulation of sperm egg fusion. Ann. Rev. Physiol. 48: 191-200.
Jaffe, L.A., Kado, R.T. and Kline, D. (1986). A calcium-activated sodium conductance produces a long-duration action potential in the egg of a nemertean worm. J. Physiol. 381: 263-278.
Kline, D., Jaffe, L.A. and Kado, R.T. (1986). A calcium-activated sodium conductance contributes to the fertilization potential in the egg of the nemertean worm Cerebratulus lacteus. Develop. Biol. 117: 184‑193.
Turner, P.R., Jaffe, L.A. and Primakoff, P. (1987). A cholera toxin-sensitive G‑protein stimulates exocytosis in sea urchin eggs. Develop. Biol. 120: 577-583.
Kline, D., Simoncini, L., Mandel, G., Maue, R., Kado, R.T. and Jaffe, L.A. (1988). Fertilization events induced by neurotransmitters after injection of mRNA in Xenopus eggs. Science 241: 464-467.
Jaffe, L.A., Turner, P.R., Kline, D., Kado, R.T. and Shilling, F. (1988). G‑proteins and egg activation. Cell Differentiation and Development 25: 15-18.
Turner, P.R. and Jaffe, L.A. (1989). G‑proteins and the regulation of oocyte maturation and fertilization. In The Cell Biology of Fertilization, eds. H. Schatten and G. Schatten. Academic Press, Orlando, pp. 297-318.
Jaffe, L.A. (1989). Receptors, G-proteins, and egg activation. In Mechanisms of Egg Activation, eds. R. Nuccitelli, G.N. Cherr and W.H. Clark, Jr. Plenum Press, N.Y., pp. 151-155.
Shilling, F., Chiba, K., Hoshi, M., Kishimoto, T. and Jaffe, L.A. (1989). Pertussis toxin inhibits 1‑methyladenine-induced maturation in starfish oocytes. Develop. Biol. 133: 605-608.
Iwao, Y. and Jaffe, L.A. (1989). Evidence that the voltage-dependent component in the fertilization process is contributed by the sperm. Develop. Biol. 134: 446-451.
Shilling, F., Mandel, G. and Jaffe, L.A. (1990). Activation by serotonin of starfish eggs expressing the rat serotonin 1c receptor. Cell Regulation (now called Molecular Biology of the Cell) 1: 465-469.
Chiba, K., Kado, R.T. and Jaffe, L.A. (1990). Development of calcium release mechanisms during starfish oocyte maturation. Develop. Biol. 140: 300-306.
Kline, D., Kado, R.T., Kopf, G.S., and Jaffe, L.A. (1990). Receptors, G-proteins, and activation of the amphibian egg. In “Mechanisms of Fertilization” (B. Dale, ed.), NATO ASI Series, volume H45, 529-541. Springer-Verlag, Berlin.
Jaffe, L.A. (1990). First messengers at fertilization. J. Reprod. Fert., supplement 42: 107-116.
Kline, D., Kopf, G.S., Muncy, L.F. and Jaffe, L.A. (1991). Evidence for the involvement of a pertussis toxin-insensitive G-protein in egg activation of the frog, Xenopus laevis. Develop. Biol. 143: 218-229.
Terasaki, M., and Jaffe, L.A. (1991). Organization of the sea urchin egg endoplasmic reticulum and its reorganization at fertilization. J. Cell Biol. 114: 929-940.
Jaffe, L.A. and Terasaki, M. (1993). Structural changes of the endoplasmic reticulum of sea urchin eggs during fertilization. Develop. Biol. 156: 566-573.
Jaffe, L.A., Gallo, C.J., Lee, R.H., Ho, Y.K., and Jones, T.L.Z. (1993). Oocyte maturation in starfish is mediated by the bg-subunit complex of a G-protein. J. Cell. Biol. 121: 775-783.
Terasaki, M., and Jaffe, L.A. (1993). Imaging the endoplasmic reticulum in living sea urchin eggs. Meth. Cell Biol. 38: 211-220.
Jaffe, L.A., and Terasaki, M. (1993). Egg activation at fertilization: structural changes in the endoplasmic reticulum. In “Meiosis II: Contemporary Approaches to the Study of Meiosis,” F. P. Haseltine and S. Heyner, eds., AAAS Press, Washington, D.C., pp. 167-174.
Jaffe, L.A. (1993). The experimental process: the electrical polyspermy block. Essay in the textbook Molecular and Cellular Biology, by Stephen L. Wolfe. Wadsworth Publishing Co., pp. 1110-1111.
Shilling, F.M., Carroll, D.J., Muslin, A.J., Escobedo, J.A., Williams, L.T., and Jaffe, L.A. (1994). Evidence for both tyrosine kinase and G-protein coupled pathways leading to starfish egg activation. Develop. Biol. 162: 590-599.
Jaffe, L.A., and Terasaki, M. (1994). Structural changes in the endoplasmic reticulum of starfish oocytes during meiotic maturation and fertilization. Develop. Biol. 164: 579-587.
Gallo, C. J., Hand, A. R., Jones, T. L. Z., and Jaffe, L. A. (1995). Stimulation of Xenopus oocyte maturation by inhibition of the G-protein as subunit, a component of the plasma membrane and yolk platelet membranes. J. Cell Biol. 130: 275-284.
Mehlmann, L. M., Terasaki, M., Jaffe, L. A., and Kline, D. (1995). Reorganization of the endoplasmic reticulum during meiotic maturation of the mouse oocyte. Develop. Biol. 170: 607-615.
Carroll, D. J., and Jaffe, L. A. (1995). Proteases stimulate fertilization-like responses in starfish eggs. Develop. Biol. 170: 690-700.
Jaffe, L.A. (1996). Egg membranes during fertilization. In “Molecular Biology of Membrane Transport Disorders,” S.G.Schultz, T. Andreoli, A. Brown, D. Fambrough, J. Hoffman, and M. Welsh, eds. Plenum Publishing Corp., New York, ch. 18, pp. 367-378.
Gallo, C.J., Jones, T.L.Z., Aragay, A.M., and Jaffe, L.A. (1996). Increased expression of Gaq family G-proteins during oocyte maturation and early development of Xenopus laevis. Develop. Biol. 177: 300-308.
Terasaki, M., Jaffe, L.A., Hunnicutt, G.R., and Hammer, J.A. III. (1996). Structural change of the endoplasmic reticulum during fertilization: evidence for loss of membrane continuity using the green fluorescent protein. Develop. Biol. 179: 320-328.
Carroll, D.J., Ramarao, C.S., Mehlmann, L.M., Roche, S., Terasaki, M., and Jaffe, L.A. (1997). Calcium release at fertilization in starfish eggs is mediated by phospholipase Cg. J. Cell Biol. 138: 1303-1311.
Williams, C.J., Mehlmann, L.M., Jaffe, L.A., Kopf, G.S., and Schultz, R.M. (1998). Evidence that Gq family G proteins do not function in mouse egg activation at fertilization. Develop. Biol. 198: 116-127.
Mehlmann, L.M., Carpenter, G., Rhee, S.G., and Jaffe. L.A. (1998). SH2 domain-mediated activation of phospholipase Cg is not required to initiate Ca2+ release at fertilization of mouse eggs. Develop. Biol. 203: 221-232.
Carroll, D.J., Albay, D.T., Terasaki, M., Jaffe, L.A., and Foltz, K.R. (1999). Identification of PLCg-dependent and independent events during fertilization of sea urchin eggs. Develop. Biol. 206: 232-247.
Giusti, A.F., Carroll, D.J., Abassi, Y.A., Terasaki, M., Foltz, K.R., and Jaffe, L.A. (1999). Requirement of a Src family kinase for initiating calcium release at fertilization in starfish eggs. J. Biol. Chem. 274: 29318-29322.
Runft, L.L., Watras, J., and Jaffe, L.A. (1999). Calcium release at fertilization of Xenopus eggs requires type I IP3 receptors, but not SH2 domain-mediated activation of PLCg or Gq-mediated activation of PLCb. Develop. Biol. 214: 399-411.
Giusti, A.F., Xu, W., Hinkle, B., Terasaki, M., and Jaffe, L.A. (2000). Evidence that fertilization activates starfish eggs by sequential activation of a Src-like kinase and phospholipase Cg. J. Biol. Chem. 275: 16788-16794
Runft, L.L., and Jaffe, L.A. (2000). Sperm extract injection into ascidian eggs signals Ca2+ release by the same pathway as fertilization. Development 127: 3227-3236.
Jaffe, L.A., Giusti, A.F., Carroll, D.J., and Foltz, K.R. (2001). Ca2+ signalling during fertilization of echinoderm eggs. Sem. Cell Develop. Biol. 12: 45-51.
Mehlmann, L.M., Chattopadhyay, A., Carpenter, G., and Jaffe, L.A. (2001). Evidence that phospholipase C from the sperm is not responsible for initiating Ca2+ release in mouse eggs at fertilization. Develop. Biol. 236: 492-501.
Runft, L.L., Jaffe, L.A., and Mehlmann, L.M. (2002). Egg activation at fertilization: where it all begins. Develop. Biol. 245: 237-254.
Mehlmann, L.M., Jones, T.L.Z., and Jaffe, L.A. (2002). Meiotic arrest in the mouse follicle maintained by a Gs protein in the oocyte. Science 297: 1343-1345.
Kalinowski, R.R., Jaffe, L.A., Foltz, K.R., and Giusti, A.F. (2003). A receptor linked to a Gi-family G-protein functions in initiating oocyte maturation in starfish but not frogs. Develop. Biol. 253: 139-149.
Giusti, A.F., O’Neill, F.J., Yamasu, K., Foltz, K.R., and Jaffe, L.A. (2003). Function of a sea urchin Src family kinase in initiating Ca2+ release at fertilization. Develop. Biol. 256: 367-378.
Kalinowski, R.R., Berlot, C.H., Jones, T.L.Z., Ross, L.F., Jaffe, L.A., and Mehlmann, L.M. (2004). Maintenance of meiotic prophase arrest in vertebrate oocytes by a Gs protein-mediated pathway. Develop. Biol. 267: 1-13.
Jaffe, L.A., and Terasaki, M. (2004). Quantitative microinjection of oocytes, eggs, and embryos. Meth. Cell Biol. 74: 219-242.
Terasaki, M., and Jaffe, L.A. (2004). Labeling of cell membranes and compartments for live cell fluorescence microscopy. Meth. Cell Biol. 74: 469-489.
Mehlmann, L.M., Saeki, Y., Tanaka, S., Brennan, T.J., Evsikov, A.V., Pendola, F.L., Knowles, B.B., Eppig, J.J., and Jaffe, L.A. (2004). The Gs-linked receptor GPR3 maintains meiotic arrest in mammalian oocytes. Science 306: 1947-1950.
Mehlmann, L.M., and Jaffe, L.A. (2005). SH2 domain-mediated activation of an SRC family kinase is not required to initiate Ca2+ release at fertilization in mouse eggs. Reproduction 129: 557-564.
Freudzon, L., Norris, R.P., Hand, A.R., Tanaka, S., Saeki, Y., Jones, T.L.Z., Rasenick, M.M., Berlot, C.H., Mehlmann, L.M., and Jaffe, L.A. (2005). Regulation of meiotic prophase arrest in mouse oocytes by GPR3, a constitutive activator of the Gs G protein. J. Cell Biol. 171: 255-265.
Mehlmann, L.M., Kalinowski, R.R., Ross, L.F., Parlow, A.F., Hewlett, E.L., and Jaffe, L.A. (2006). Meiotic resumption in response to luteinizing hormone is independent of a Gi family G protein or calcium in the mouse oocyte. Develop. Biol. 299: 345-355.
Norris, R.P., Freudzon, L., Freudzon, M., Hand, A.R., Mehlmann, L.M., and Jaffe, L.A. (2007). A Gs-linked receptor maintains meiotic arrest in mouse oocytes, but luteinizing hormone does not cause meiotic resumption by terminating receptor-Gs signaling. Develop. Biol. 310:240-249.
Norris, R.P., Freudzon, M., Mehlmann, L.M., Cowan, A.E., Simon, A.M., Paul, D.L., Lampe, P.D., and Jaffe, L.A. (2008). Luteinizing hormone causes MAPK-dependent phosphorylation and closure of Cx43 gap junctions in mouse ovarian follicles: one of two paths to meiotic resumption. Development 135:3229-3238.
Jaffe, L.A., Norris, R.P., Freudzon, M., Ratzan, W.J., and Mehlmann, L.M. (2009). Microinjection of follicle-enclosed mouse oocytes. Methods Mol. Biol. 518:157-173.
Norris, R.P., Ratzan, W.J., Freudzon, M., Mehlmann, L.M., Krall, J., Movsesian, M.A., Wang, H., Ke, H., Nikolaev, V.O., and Jaffe, L.A. (2009). Cyclic GMP from the surrounding somatic cells regulates cyclic AMP and meiosis in the mouse oocyte. Development 136:1869-1878.
Jaffe, L.A., and Norris, R.P. (2010). Initiation of the meiotic prophase-to-metaphase transition in mammalian oocytes. In Oogenesis — The Universal Process, ed. M.-H. Verlhac and A. Villeneuve. John Wiley & Sons, Ltd, London, pp. 181-197.
Norris, R.P., Freudzon, M., Nikolaev, V.O., and Jaffe, L.A. (2010). EGF receptor kinase activity is required for gap junction closure and for part of the decrease in ovarian follicle cGMP in response to luteinizing hormone. Reproduction 140: 655-662.
Ratzan, W.J., Evsikov, A.V., Okamura, Y., and Jaffe, L.A. (2011). Voltage sensitive phosphoinositide phosphatases of Xenopus: their tissue distribution and voltage dependence. J. Cell. Physiol. 226: 2740-2746.
Robinson, J.W., Zhang, M., Shuhaibar, L.C., Norris, R.P., Geerts, A., Wunder, F., Eppig, J.J., Potter, L.R., and Jaffe, L.A. (2012). Luteinizing hormone reduces the activity of the NPR2 guanylyl cyclase in mouse ovarian follicles, contributing to the cyclic GMP decrease that promotes resumption of meiosis in oocytes. Develop. Biol. 366: 308-316.
Lee, K.-B., Zhang, M., Sugiura, K., Wigglesworth, K., Uliasz, T., Jaffe, L.A., and Eppig, J.J. (2013). Hormonal coordination of natriuretic peptide type C and natriuretic peptide receptor 3 expression in mouse granulosa cells. Biol. Reprod. 88: 42, 1-9.
Egbert, J.R., Shuhaibar, L.C., Edmund, A.B., Van Helden, D.A., Robinson, J.W., Uliasz, T.F., Baena, V., Geerts, A., Wunder, F., Potter, L.R., and Jaffe, L.A. (2014). Dephosphorylation and inactivation of the NPR2 guanylyl cyclase in the granulosa cells contributes to the LH-induced cGMP decrease that causes meiotic resumption in rat oocytes. Development 141: 3594-3604.
Shuhaibar, L.C., Egbert, J.R., Norris, R.P., Lampe, P.D., Nikolaev, V.O., Thunemann, M., Wen, L., Feil, R., and Jaffe, L.A. (2015). Intercellular signaling via cyclic GMP diffusion through gap junctions restarts meiosis in mouse ovarian follicles. Proc. Natl. Acad. Sci. USA 112: 5527-5532.
Shuhaibar, L.C., Egbert, J.R., Edmund, A.B., Uliasz, T.F., Dickey, D.M., Yee, S.-P., Potter, L.R., and Jaffe, L.A. (2016) Dephosphorylation of juxtamembrane serines and threonines of the NPR2 guanylyl cyclase is required for rapid resumption of oocyte meiosis in response to luteinizing hormone. Develop. Biol. 409:194-201.
Egbert J.R., Uliasz T.F., Shuhaibar, L.C., Geerts A., Wunder F., Kleiman R.J., Humphrey J.M., Lampe P.D., Artemyev, N.O., Rybalkin, S.D., Beavo, J.A., Movsesian, M.A., and Jaffe, L.A. (2016). Luteinizing hormone causes phosphorylation and activation of the cyclic GMP phosphodiesterase PDE5 in rat ovarian follicles, contributing, together with PDE1 activity, to the resumption of meiosis. Biol. Reprod. 94(5):110.
Jaffe, L.A., and Egbert J.R. (2017). Regulation of mammalian oocyte meiosis by intercellular communication within the ovarian follicle. Ann. Rev. Physiol. 79:237-260.
Robinson, J.W., Egbert, J.R., Davydova, J., Schmidt, H., Jaffe, L.A., and Potter, L.R. (2017). Dephosphorylation is the mechanism of fibroblast growth factor inhibition of guanylyl cyclase-B. Cellular Signaling 40:222-229.
Shuhaibar, L.C., Robinson, J.W., Vigone, G., Shuhaibar, N.P., Egbert, J.R., Baena, V., Uliasz, T.V., Kaback, D., Yee, S.-P., Feil, R., Fisher, M.C., Dealy, C.N., Potter, L.R., and Jaffe, LA. (2017). Dephosphorylation of the NPR2 guanylyl cyclase contributes to inhibition of bone growth by fibroblast growth factor. eLife 6:e31343.
Egbert, J.R., Yee, S.-P., and Jaffe, L.A. (2018). Luteinizing hormone signaling phosphorylates and activates the cyclic GMP phosphodiesterase PDE5 in mouse ovarian follicles, contributing an additional component to the hormonally induced decrease in cyclic GMP that reinitiates meiosis. Develop. Biol. 435:6-14.
Jaffe, L.A. (2018). Shedding light on spawning in jellyfish. eLife 7:e34258.
Vigone G., Shuhaibar L.C., Egbert, J.R., Uliasz, T.F., Movsesian, M.A., and Jaffe, L.A. (2018). Multiple cAMP phosphodiesterases act together to prevent premature oocyte meiosis and ovulation. Endocrinology 5:2142-2152.
Shuhaibar, L.C., Carroll, D.J., and Jaffe, L.A. (2018). Preparing for fertilization: Intercellular signals for oocyte maturation. In “Reproductive and Developmental Strategies” (K. Kobayashi, T. Kitano, Y. Iwao, and M. Kondo, eds), Springer, Japan, chapter 25, pp. 535-548.
Jaffe, L.A. (2018). The fast block to polyspermy: New insight into a century old problem. J. Gen. Physiol. 150:1233-1234.
Egbert, J.R., Fahey, P.G., Reimer, J., Owen, C.M., Evsikov, A.V., Nikolaev, V.O., Griesbeck, O., Ray, R.S., Tolias, A.S., and Jaffe, L.A. (2019). Follicle-stimulating hormone and luteinizing hormone increase Ca2+ in the granulosa cells of mouse ovarian follicles. Biol. Reprod. 101:433-444.