A research position is now available for a student, postdoctoral fellow or technical assistant.
This research position is funded by a recent NIH grant exploring genetically encoded indicators of neuronal functions.
Experiments will be based on breeding, genotyping, electroporation, patch electrode recordings, calcium imaging, voltage-sensitive dye recordings and computer simulations (modeling).
Highly motivated, completely-devoted-to-science and hardworking individuals are encouraged to apply by sending their letter of inquiry to Srdjan Antic.
Filtering of action potentials in the apical tuft. D1) Microphotograph of a layer 5 pyramidal neuron in the rat prefrontal cortex. The neuron is filled with two fluorescent dyes (AlexaFluor 594 and Oregon Green Bapta-1). D2) Three action potentials were evoked by somatic current injections. Optical imaging of action potential-induced dendritic calcium transients was performed simultaneously from 7 locations (1 - 7) marked in D1. Bottom trace represents whole-cell recording from the cell body (soma). Three action potentials back propagate into the apical tuft dendrites and induce calcium transients. At each branch point (BP-2 to BP-4), one late AP is lost. Note that 3 spikes start from location number 1. The third spike is lost after passing through branch point BP-2. Two spikes start from location number 3. The second spike is lost after passing through branch point BP-4, so that only the first spike arrives in locations numbers 5, 6 and 7. Published in Neurophotonics.