Publications

Syphilis/Treponema Pallidum

Wentao Chen, David Šmajs, Yongfei Hu, Wujian Ke, Petra Pospíšilová, Kelly L Hawley, Melissa J Caimano, Justin D Radolf, Arlene Sena, Joseph D Tucker, Bin Yang, Jonathan J Juliano, Heping Zheng, Jonathan B Parr. Analysis of Treponema pallidum strains from China using improved methods for whole-genome sequencing from primary syphilis chancres. J Infect Dis. 2020 Jul 25 https://pubmed.ncbi.nlm.nih.gov/32710788/

Amit Luthra, Jairo M Montezuma-Rusca, Carson J La Vake, Morgan LeDoyt, Kristina N Delgado, Timothy C Davenport, Mary Fiel-Gan, Melissa J Caimano, Justin D Radolf, Kelly L Hawley. Evidence that immunization with TP0751, a bipartite Treponema pallidum lipoprotein with an intrinsically disordered region and lipocalin fold, fails to protect in the rabbit model of experimental syphilis. PLoS Pathog. 2020 Sep 16; 16(9). https://pubmed.ncbi.nlm.nih.gov/32936831/

Kumar S, Caimano MJ,Anand A,Dey A,Hawley KL, LeDoyt M, La Vake C, Cruz AR, Ramirez LG, Pastekova L, Bezsonova I, Smajs D, Salazar JS, Radolf JD.Sequence variation of rare outer membrane proteins Beta-barrel domains in clinical strains provide insights into the evolution of Treponema pallidum subsp. pallidum, the syphilis spirochete. MBio. 2018.https://www.ncbi.nlm.nih.gov/pubmed/?term=Kumar+caimano+sequence+variation

Trujillo R, MD, Cervantes J, MD, PhD, Hawley KL, PhD, Cruz AR, MD, Babapoor S, DVM, PhD, MB(ASCP), Murphy M, MB, BCh, BAO, MMedSc, (NUI), Dadras SS, MD, PhD, and Salazar JC, MD, MPH. Inflammation and immune evasion coexist in Treponema pallidumeinfected skin. JAAD Case Reports 2018;4:462-4.https://www.ncbi.nlm.nih.gov/pubmed/29984283

Hawley KL, Cruz AR, Benjamin SJ, La Vake CJ, Cervantes JL, LeDoyt M, Ramirez LG, Mandich D, Fiel-Gan M, Caimano MJ, Radolf JR and Salazar JC. IFNγ Enhances CD64-Potentiated Phagocytosis of Treponema pallidum Opsonized with Human Syphilitic Serum by Human Macrophages. Front Immunol. 2017 Oct 5;8:1227. doi: 10.3389/fimmu.2017.01227. eCollection 2017. https://www.ncbi.nlm.nih.gov/pubmed/29051759

Seña AC, Zhang XH, Li T, Zheng HP, Yang B, Yang LG, Salazar JC, Cohen MS, Moody MA, Radolf JD, Tucker JD. A systematic review of syphilis serological treatment outcomes in HIV-infected and HIV-uninfected persons: rethinking the significance of serological non-responsiveness and the serofast state after therapy. BMC Infect Dis. 2015 Oct 28; 15:479. https://www.ncbi.nlm.nih.gov/pubmed/26511465

Luthra A, Anand A, Hawley KL, LeDoyt M, La Vake CJ, Caimano MJ, Cruz AR, Salazar JC, Radolf JD. A Homology Model Reveals Novel Structural Features and an Immunodominant Surface Loop/Opsonic Target in the Treponema pallidum BamA Ortholog TP_0326. J Bacteriol. 2015 Jun;197(11):1906-20. https://www.ncbi.nlm.nih.gov/pubmed/25825429

Cruz AR, Castrillón MA, Minotta AY, Rubiano LC, Castaño MC, Salazar JC. Gestational and congenital syphilis epidemic in the Colombian Pacific Coast. Sex Transm Dis. 2013 Oct;40(10):813-8. https://www.ncbi.nlm.nih.gov/pubmed/24275735

Silver AC, Dunne DW, Zeiss CJ, Bockenstedt LK, Radolf JD, Salazar JC, and Fikrig. MyD88 deficiency markedly worsens tissue inflammation and bacterial clearance in mice infected with Treponema pallidum, the agent of syphilis. PLoS One 2013 Aug 5; 8(8). http://www.ncbi.nlm.nih.gov/pubmed/23940747.

Cruz AR, Ramirez LG, Zuluaga AV, Pillay A, Abreu C, Valencia CA, La Vake C, Cervantes JL, Dunham-Ems S, Cartun R, Mavilio D, Radolf JD, Salazar JC. Immune evasion and recognition of the syphilis spirochete in blood and skin of secondary syphilis patients: two immunologically distinct compartments. PLoS Negl Trop Dis. 2012; 6(7). https://www.ncbi.nlm.nih.gov/pubmed/22816000

Anand A, Luthra A, Dunham-Ems S, Caimano MJ, Karanian C, LeDoyt M, Cruz AR, Salazar JC, Radolf JD. TprC/D (Tp0117/131), a trimeric, pore-forming rare outer membrane protein of Treponema pallidum, has a bipartite domain structure. J Bacteriol. 2012 May; 194(9):2321-33. https://www.ncbi.nlm.nih.gov/pubmed/22389487

Desrosiers DC, Anand A, Luthra A, Dunham-Ems SM, LeDoyt M, Cummings MA, Eshghi A, Cameron CE, Cruz AR, Salazar JC, Caimano MJ, Radolf JD. TP0326, a Treponema pallidum β-barrel assembly machinery A (BamA) orthologue and rare outer membrane protein. Mol Microbiol. 2011 Jun; 80(6):1496-515. https://www.ncbi.nlm.nih.gov/pubmed/21488980

Cox DL, Luthra A, Dunham-Ems S, Desrosiers DC, Salazar JC, Caimano MJ, Radolf JD. Surface immunolabeling and consensus computational framework to identify candidate rare outer membrane proteins of Treponema pallidum. Infect Immun. 2010 Dec; 78(12):5178-94. https://www.ncbi.nlm.nih.gov/pubmed/20876295

Cruz AR, Pillay A, Zuluaga AV, Ramirez LG, Duque JE, Aristizabal GE, Fiel-Gan MD, Jaramillo R, Trujillo R, Valencia C, Jagodzinski L, Cox DL, Radolf JD, Salazar JC. Secondary syphilis in cali, Colombia: new concepts in disease pathogenesis. PLoS Negl Trop Dis. 2010 May 18; 4(5). https://www.ncbi.nlm.nih.gov/pubmed/20502522

Lomotey CJ, Lewis J, Gebrian B, Bourdeau R, Dieckhaus K, Salazar JC. Maternal and congenital syphilis in rural Haiti. Rev Panam Salud Publica. 2009 Sep; 26(3):197-202. https://www.ncbi.nlm.nih.gov/pubmed/20058828

Salazar JC, Cruz AR, Pope CD, Valderrama L, Trujillo R, Saravia NG, Radolf JD. Treponema pallidum elicits innate and adaptive cellular immune responses in skin and blood during secondary syphilis: a flow-cytometric analysis. J Infect Dis. 2007 Mar 15; 195(6):879-87. https://www.ncbi.nlm.nih.gov/pubmed/17299719

Lyme Disease/Borrelia Burgdorferi

Melissa J Caimano, Ashley M Groshong, Alexia Belperron, Jialing Mao, Kelly L Hawley, Amit Luthra, Danielle E Graham, Christopher G Earnhart, Richard T Marconi, Linda K Bockenstedt, Jon S Blevins, Justin D Radolf. The RpoS Gatekeeper in Borrelia burgdorferi: An Invariant Regulatory Scheme That Promotes Spirochete Persistence in Reservoir Hosts and Niche Diversity. Front Microbiol. 2019 Aug 21; 10:1923 https://pubmed.ncbi.nlm.nih.gov/31507550/

Cervantes JL, Hawley KL, Benjamin SJ, Weinerman B, Luu SM, Salazar JC. Phagosomal TLR signaling upon Borrelia burgdorferi infection. Front Cell Infect Microbiol. 2014 May 20; 4:55. Review https://www.ncbi.nlm.nih.gov/pubmed/24904837

Cervantes, JL, La Vake, CJ, Weinerman, B, Luu, S, O'Connell, C, Verardi, PH, Salazar, JC. Human TLR8 is activated upon recognition of Borrelia burgdorferi RNA in the phagosome of human monocytes. J. Leuk. Biol. 2013; 94:1-11. http://www.ncbi.nlm.nih.gov/pubmed/23906644.

Cervantes JL, Weinerman B, Basole C, Salazar JC. TLR8: the forgotten relative revindicated. Cell Mol Immunol. 2012 Nov; 9(6):434-8. Review. https://www.ncbi.nlm.nih.gov/pubmed/23085951

Hawley KL, Olson CM Jr, Iglesias-Pedraz JM, Navasa N, Cervantes JL, Caimano MJ, Izadi H, Ingalls RR, Pal U, Salazar JC, Radolf JD, Anguita J. CD14 cooperates with complement receptor 3 to mediate MyD88-independent phagocytosis of Borrelia burgdorferi. Proc Natl Acad Sci U S A. 2012 Jan 24; 109(4):1228-32. https://www.ncbi.nlm.nih.gov/pubmed/22232682

Cervantes JL, Dunham-Ems SM, La Vake CJ, Petzke MM, Sahay B, Sellati TJ, Radolf JD, Salazar JC. Phagosomal signaling by Borrelia burgdorferi in human monocytes involves Toll-like receptor (TLR) 2 and TLR8 cooperativity and TLR8-mediated induction of IFN-beta. Proc Natl Acad Sci U S A. 2011 Mar 1; 108(9):3683-8. https://www.ncbi.nlm.nih.gov/pubmed/21321205

Sahay B, Patsey RL, Eggers CH, Salazar JC, Radolf JD, Sellati TJ. CD14 signaling restrains chronic inflammation through induction of p38-MAPK/SOCS-dependent tolerance. PLoS Pathog. 2009 Dec; 5(12) https://www.ncbi.nlm.nih.gov/pubmed/20011115

Salazar JC, Duhnam-Ems S, La Vake C, Cruz AR, Moore MW, Caimano MJ, Velez-Climent L, Shupe J, Krueger W, Radolf JD. Activation of human monocytes by live Borrelia burgdorferi generates TLR2-dependent and -independent responses which include induction of IFN-beta. PLoS Pathog. 2009 May; 5(5). https://www.ncbi.nlm.nih.gov/pubmed/19461888

Cruz AR, Moore MW, La Vake CJ, Eggers CH, Salazar JC, Radolf JD. Phagocytosis of Borrelia burgdorferi, the Lyme disease spirochete, potentiates innate immune activation and induces apoptosis in human monocytes. Infect Immun. 2008 Jan; 76(1):56-70. https://www.ncbi.nlm.nih.gov/pubmed/17938216

Moore MW, Cruz AR, LaVake CJ, Marzo AL, Eggers CH, Salazar JC, Radolf JD. Phagocytosis of Borrelia burgdorferi and Treponema pallidum potentiates innate immune activation and induces gamma interferon production. Infect Immun. 2007 Apr; 75(4):2046-62. https://www.ncbi.nlm.nih.gov/pubmed/17220323

Other Research Publications

Qiu Z, Cervantes JL, Cicek BB, Mukherjee S, Venkatesh M, Maher LA, Salazar JC, Mani S, Khanna KM. Pregnane X Receptor Regulates Pathogen-Induced Inflammation and Host Defense against an Intracellular Bacterial Infection through Toll-like Receptor 4. Sci Rep. 2016 Aug 23; 6:31936. https://www.ncbi.nlm.nih.gov/pubmed/27550658

Libardo MD, Cervantes JL, Salazar JC, Angeles-Boza AM. Improved bioactivity of antimicrobial peptides by addition of amino-terminal copper and nickel (ATCUN) binding motifs. ChemMedChem. 2014 Aug; 9(8):1892-901. https://www.ncbi.nlm.nih.gov/pubmed/24803240

Salazar JC, Cahn P, Della Negra M, De Aquino MZ, Robinson PA, Jelaska A, Mikl J. Efficacy and safety of tipranavir coadministered with ritonavir in HIV-1-infected children and adolescents: 5 years of experience. Pediatr Infect Dis J. 2014 Apr; 33(4):396-400. https://www.ncbi.nlm.nih.gov/pubmed/23995585

Abzug MJ, Song LY, Levin MJ, Nachman SA, Borkowsky W, Pelton SI; International Maternal Pediatric Adolescent AIDS Clinical Trials Group P1024 and P1061s Protocol Teams. Antibody persistence and immunologic memory after sequential pneumococcal conjugate and polysaccharide vaccination in HIV-infected children on highly active antiretroviral therapy. Vaccine. 2013 Oct 1; 31(42):4782-90. https://www.ncbi.nlm.nih.gov/pubmed/23954381

Clark RB, Cervantes JL, Maciejewski MW, Farrokhi V, Nemati R, Yao X, Anstadt E, Fujiwara M, Wright KT, Riddle C, La Vake CJ, Salazar JC, Finegold S, Nichols FC. Serine lipids of Porphyromonas gingivalis are human and mouse Toll-like receptor 2 ligands. Infect Immun. 2013 Sep; 81(9):3479-89. https://www.ncbi.nlm.nih.gov/pubmed/23836823

Roehm CE, Salazar JC, Hagstrom N, Valdez TA. Phoma and Acremonium invasive fungal rhinosinusitis in congenital acute lymphocytic leukemia and literature review. Int J Pediatr Otorhinolaryngol. 2012 Oct; 76(10):1387-91. https://www.ncbi.nlm.nih.gov/pubmed/22818128

Patel K, Van Dyke RB, Mittleman MA, Colan SD, Oleske JM, Seage GR 3rd; International Maternal Pediatric Adolescent AIDS Clinical Trials 219219C Study Team. The impact of HAART on cardiomyopathy among children and adolescents perinatally infected with HIV-1. AIDS. 2012 Oct 23; 26(16):2027-37. https://www.ncbi.nlm.nih.gov/pubmed/22781228

Abzug MJ, Qin M, Levin MJ, Fenton T, Beeler JA, Bellini WJ, Audet S, Sowers SB, Borkowsky W, Nachman SA, Pelton SI, Rosenblatt HM; International Maternal Pediatric Adolescent AIDS Clinical Trials Group P1024 and P1061s Protocol Teams. Immunogenicity, immunologic memory, and safety following measles revaccination in HIV-infected children receiving highly active antiretroviral therapy. J Infect Dis. 2012 Aug 15; 206(4):512-22. https://www.ncbi.nlm.nih.gov/pubmed/22693229

Stojanov S, Lapidus S, Chitkara P, Feder H, Salazar JC, Fleisher TA, Brown MR, Edwards KM, Ward MM, Colbert RA, Sun HW, Wood GM, Barham BK, Jones A, Aksentijevich I, Goldbach-Mansky R, Athreya B, Barron KS, Kastner DL. Periodic fever, aphthous stomatitis, pharyngitis, and adenitis (PFAPA) is a disorder of innate immunity and Th1 activation responsive to IL-1 blockade. Proc Natl Acad Sci U S A. 2011 Apr 26; 108(17):7148-53. https://www.ncbi.nlm.nih.gov/pubmed/21478439

Courter JD, Teevan CJ, Li MH, Girotto JE, Salazar JC. Role of tipranavir in treatment of patients with multidrug-resistant HIV. Ther Clin Risk Manag. 2010 Oct 5; 6:431-41. https://www.ncbi.nlm.nih.gov/pubmed/20957134

Desrosiers DC, Bearden SW, Mier I Jr, Abney J, Paulley JT, Fetherston JD, Salazar JC, Radolf JD, Perry RD. Znu is the predominant zinc importer in Yersinia pestis during in vitro growth but is not essential for virulence. Infect Immun. 2010 Dec; 78(12):5163-77. https://www.ncbi.nlm.nih.gov/pubmed/20855510

Deville JG, Goldfarb J, Kaplan SL, Equils O, Huang DB, Ang JY, Salazar JC. The use of linezolid in the treatment of paediatric patients with infections caused by enterococci including strains resistant to vancomycin. J Antimicrob Chemother. 2010 Oct; 65(10):2267-70. https://www.ncbi.nlm.nih.gov/pubmed/20705627

Kanev PM, Salazar JC. Unusual CNS infection from a subtorcular dermal sinus. Acta Paediatr. 2010 Apr; 99(4):627-9. https://www.ncbi.nlm.nih.gov/pubmed/19912143

Feder Jr. HM and Salazar JC. A clinical review of 105 patients with PFAPA (A periodic fever syndrome). Acta Paediatr. 2010 Feb; 99(2):178-84. https://www.ncbi.nlm.nih.gov/pubmed/19889105

Nachman SA, Chernoff M, Gona P, Van Dyke RB, Dankner WM, Seage GR 3rd, Oleske J, Williams PL; PACTG 219C Team. Incidence of noninfectious conditions in perinatally HIV-infected children and adolescents in the HAART era. Arch Pediatr Adolesc Med. 2009 Feb; 163(2):164-71. https://www.ncbi.nlm.nih.gov/pubmed/19188649

Van Dyke RB, Wang L, Williams PL; Pediatric AIDS Clinical Trials Group 219C Team. Toxicities associated with dual nucleoside reverse-transcriptase inhibitor regimens in HIV-infected children. J Infect Dis. 2008 Dec 1; 198(11):1599-608. https://www.ncbi.nlm.nih.gov/pubmed/19000014

Patel K, Hernán MA, Williams PL, Seeger JD, McIntosh K, Dyke RB, Seage GR 3rd; Pediatric AIDS Clinical Trials Group 219/219C Study Team. Long-term effects of highly active antiretroviral therapy on CD4+ cell evolution among children and adolescents infected with HIV: 5 years and counting. Clin Infect Dis. 2008 Jun 1; 46(11):1751-60. https://www.ncbi.nlm.nih.gov/pubmed/18426371

Williams PL, Van Dyke R, Eagle M, Smith D, Vincent C, Ciupak G, Oleske J, Seage GR 3rd; PACTG 219C Team. Association of site-specific and participant-specific factors with retention of children in a long-term pediatric HIV cohort study. Am J Epidemiol. 2008 Jun 1; 167(11):1375-86. https://www.ncbi.nlm.nih.gov/pubmed/18413359

Tassiopoulos K, Williams PL, Seage GR 3rd, Crain M, Oleske J, Farley J; International Maternal Pediatric Adolescent AIDS Clinical Trials 219C Team. Association of hypercholesterolemia incidence with antiretroviral treatment, including protease inhibitors, among perinatally HIV-infected children. J Acquir Immune Defic Syndr. 2008 Apr 15; 47(5):607-14. https://www.ncbi.nlm.nih.gov/pubmed/18209684

Book Chapters

Salazar JC and Feder HM, Periodic Fever Syndromes. Chapter 176, in Textbook of Pediatric Dermatology, 3rd Edition, Edited by A. Irvine, P. Hoeger and A. Yan, 2010, Blackwell Publishing Ltd.

Salazar and Hawley Laboratory

Syphilis/Treponema Pallidum

Lyme Disease/Borrelia Burgdorferi

Other Research Publications

Book Chapters