All are rabbit polyclonals; performance of many is improved by affinity-purification.

Requesting Neuropeptide Scientific Materials

Antigen	Identification	Usage	Affinity-purification	Reference
Peptides
ACTH(18-39)	JH44	RIA; Westerns; immunostain	no	Schnabel et al 1989 Mol Endo 3:1223
ACTH(18-39)	Kathy	RIA; immunostain	no	Mains&Eipper 1983 Endocrinology 112:1986
g3MSH	JH189	Westerns	yes	Noel et al 1991 Mol Endo 5:404
POMC N-term	Georgie	Westerns, immunostaining	no; use 5% milk in Westerns	Mains&Eipper 1979 J Biol Chem 254:7885
NPY	JH3	Immunostaining	no	Dickerson et al 1987 J Biol Chem 262:13646
PAM
Rat PHM	JH1761	Westerns	yes	Oyarce et al 2001 J Biol Chem 276:33265
Rat PHM	JH475	Westerns, IPT	no	Yun et al Arch Biochem Biophys 1993 301:77
rPAM(116-131)	JH246	Western	yes	Eipper et al 1991 J Biol Chem 266:7827
bPAM(288-310)	JH100	Western	yes	Husten&Eipper 1991 J Biol Chem 266:17004
rPAM exon 16	JH629	Western, IPT, immunostaining	yes	Maltese&Eipper 1992 Mol Endo 6:1998
bPAM(561-579)	JH69	Westerns	yes	Husten&Eipper 1991 J Biol Chem 266:17004
rPAL	JH471	Western	no	Yun et al Arch Biochem Biophys 1993 301:77
rPAL	JH877	Western	no	Kolhekar et al 2002 Biochemistry 41:12384
rPAM C-term	CT267,268	Western, IPT	yes	Francone et al 2010 Mol Endo 24:1543
rPAM-(p)S937	JH1922	Western		Steveson et al 1999 J Biol Chem 274:21128
rPAM-CKII site	JH2540-42	Western		Steveson et al 2001 J Biol Chem 276:40326
rPAM C-term	6E6  (mAb)	Western, Immunostain	No need	Milgram et al 1997 J Cell Sci 110:695

Kalirin Kalirin, SR4-7 JH2580/1/2 Western, IPT yes Penzes et al 2000 J Biol Chem 275:7395 Kalirin, Sec14 CT301/302 Western, IPT yes Miller et al 2015 J Biol Chem 290:13541 Kalirin-7 C-term JH2958/9 Western, IPT, immunostaining No; very inefficient Penzes et al 2000 J Biol Chem 275:7395 Kalirin-12 JH3225/7 Johnson et al 2000 J Biol Chem 275:19324 Trio Trio SR5 CT232/233 McPherson et al 2005 Gene 347:125 hTrio Sec14 CT320/21/22 Katrancha et al submitted hTrio IgFnIII CT217, 219 Katrancha et al submitted Others Atp7a CT77/78 Western, immunostaining yes Steveson et al 2003 Endocrinology 144:188 RESP18 JH1162/3 Western, immunostaining no Schiller et al 1995 J Biol Chem 270:26129 rDBM JH3125/6 Western no Xin et al 2004 J Biol Chem 279:48159 PC1 JH888 Western, immunostaining yes Zhou&Mains 1994 J Biol Chem 269:17440 PC2 JH1125/6 Western yes Zhou&Mains 1994 J Biol Chem 269:17440