All are rabbit polyclonals; performance of many is improved by affinity-purification.
Requesting Neuropeptide Scientific Materials
Antigen | Identification | Usage | Affinity-purification | Reference |
Peptides | ||||
ACTH(18-39) | JH44 | RIA; Westerns; immunostain | no | Schnabel et al 1989 Mol Endo 3:1223 |
ACTH(18-39) | Kathy | RIA; immunostain | no | Mains&Eipper 1983 Endocrinology 112:1986 |
g3MSH | JH189 | Westerns | yes | Noel et al 1991 Mol Endo 5:404 |
POMC N-term | Georgie | Westerns, immunostaining | no; use 5% milk in Westerns | Mains&Eipper 1979 J Biol Chem 254:7885 |
NPY | JH3 | Immunostaining | no | Dickerson et al 1987 J Biol Chem 262:13646 |
PAM | ||||
Rat PHM | JH1761 | Westerns | yes | Oyarce et al 2001 J Biol Chem 276:33265 |
Rat PHM | JH475 | Westerns, IPT | no | Yun et al Arch Biochem Biophys 1993 301:77 |
rPAM(116-131) | JH246 | Western | yes | Eipper et al 1991 J Biol Chem 266:7827 |
bPAM(288-310) | JH100 | Western | yes | Husten&Eipper 1991 J Biol Chem 266:17004 |
rPAM exon 16 | JH629 | Western, IPT, immunostaining | yes | Maltese&Eipper 1992 Mol Endo 6:1998 |
bPAM(561-579) | JH69 | Westerns | yes | Husten&Eipper 1991 J Biol Chem 266:17004 |
rPAL | JH471 | Western | no | Yun et al Arch Biochem Biophys 1993 301:77 |
rPAL | JH877 | Western | no | Kolhekar et al 2002 Biochemistry 41:12384 |
rPAM C-term | CT267,268 | Western, IPT | yes | Francone et al 2010 Mol Endo 24:1543 |
rPAM-(p)S937 | JH1922 | Western | Steveson et al 1999 J Biol Chem 274:21128 | |
rPAM-CKII site | JH2540-42 | Western | Steveson et al 2001 J Biol Chem 276:40326 | |
rPAM C-term | 6E6 (mAb) | Western, Immunostain | No need | Milgram et al 1997 J Cell Sci 110:695 |
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