Genome Editing
The Cell and Genome Engineering Core (CGEC) supports the university research community by providing state-of-the-art genome editing services and scientific expertise. Our mission is to empower investigators with high-quality, customized cellular models that accelerate discovery and innovation.
We offer full-service TALEN and CRISPR/Cas9 strategies to engineer cell lines for a wide variety of research applications. Our capabilities include:
- Precise single–base pair edits to introduce or correct patient-specific mutations
- Targeted deletions, ranging from a few bases to multi-megabase regions, for knockout model generation
- Reporter and fusion-protein knock-ins at any genomic locus
- Safe-harbor locus engineering to support reliable expression of exogenous DNA
- Lentiviral delivery options for genome editing in hard-to-transfect or suspension cancer cell lines
Knockouts and Targeted Deletions
- Targeted sequence deletions ranging from a few bases to multi-megabase regions
- Knockout generation in hard-to-transfect cancer cell lines using lentiviral delivery systems
Knock-ins and Reporter Engineering
- Targeted knock-in of reporters, fusion tags, and functional elements
- Design and integration of custom reporter constructs
- Safe-harbor locus engineering for stable and consistent expression of exogenous DNA
We specialize in scarless genome editing using optimized single-stranded or HDR-based templates. For custom reporter constructs, we can design, build, and target vectors tailored to your laboratory’s research needs.
Contact us at ucscicore@uchc.edu to discuss your project and explore how we can support your scientific goals.
Additional information:
Genome Editing Timeline
