{"id":190,"date":"2017-10-31T13:17:24","date_gmt":"2017-10-31T17:17:24","guid":{"rendered":"https:\/\/health.uconn.edu\/structural-biology\/?page_id=190"},"modified":"2017-11-03T15:33:57","modified_gmt":"2017-11-03T19:33:57","slug":"catalytic-domain-of-%ce%b3%ce%b4-resolvase","status":"publish","type":"page","link":"https:\/\/health.uconn.edu\/structural-biology\/protein-structures\/catalytic-domain-of-%ce%b3%ce%b4-resolvase\/","title":{"rendered":"Catalytic Domain of \u03b3\u03b4 Resolvase"},"content":{"rendered":"<div id=\"pl-190\"  class=\"panel-layout\" ><div id=\"pg-190-0\"  class=\"panel-grid panel-no-style\" ><div id=\"pgc-190-0-0\"  class=\"panel-grid-cell\" ><div id=\"panel-190-0-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"0\" ><div class=\"textwidget\"><p><div style=\"width: 400px;\" class=\"wp-video\"><!--[if lt IE 9]><script>document.createElement('video');<\/script><![endif]-->\n<video class=\"wp-video-shortcode\" id=\"video-190-1\" width=\"400\" height=\"360\" loop=\"1\" autoplay=\"1\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"https:\/\/health.uconn.edu\/structural-biology\/wp-content\/uploads\/sites\/177\/2017\/11\/Catalytic-Resolvase-1.mp4?_=1\" \/><a href=\"https:\/\/health.uconn.edu\/structural-biology\/wp-content\/uploads\/sites\/177\/2017\/11\/Catalytic-Resolvase-1.mp4\">https:\/\/health.uconn.edu\/structural-biology\/wp-content\/uploads\/sites\/177\/2017\/11\/Catalytic-Resolvase-1.mp4<\/a><\/video><\/div><\/p>\n<\/div><\/div><\/div><div id=\"pgc-190-0-1\"  class=\"panel-grid-cell\" ><div class=\"panel-cell-style panel-cell-style-for-190-0-1\" ><div id=\"panel-190-0-1-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"1\" ><div class=\"textwidget\"><p>Site-specific DNA recombination allows for controlled rearrangement of DNA segments to provide genetic diversity or a selective advantage for the organism. \u03b3\u03b4 resolvase is a site-specific DNA recombinase that catalyzes the final stage of the \u03b3\u03b4 DNA transposition in E. coli to form two catenated circular DNA products. \u03b3\u03b4 resolvase is a 20 kDa homodimer comprising a catalytic domain and C-terminal dimerization interface. The structure shown here is that of the monomeric catalytic domain which comprises a four-stranded parallel b sheet surrounded by three a helices.<\/p>\n<hr \/>\n<p>Pan, B., Maciejewski, M.W., Marintchev, A. and Mullen, G.P. (2001), Solution structure of the catalytic domain of \u03b3\u03b4 resolvase. Implications for the mechanism of catalysis.\u00a0<em>Journal of Molecular Biology<\/em>\u00a0310, 1089-1107.<\/p>\n<p><a href=\"http:\/\/www.ncbi.nlm.nih.gov\/entrez\/query.fcgi?cmd=Retrieve&amp;db=PubMed&amp;list_uids=11501998&amp;dopt=Abstract\">PubMed<\/a>\u00a0|\u00a0<a href=\"http:\/\/www.idealibrary.com\/retrieve\/doi\/10.1006\/jmbi.2001.4821\" class=\"broken_link\">Journal<\/a>\u00a0|\u00a0<a href=\"http:\/\/www.rcsb.org\/pdb\/cgi\/explore.cgi?pid=81701037811244&amp;pdbId=1GHT\" class=\"broken_link\">PDB Link<\/a><\/p>\n<p><a href=\"https:\/\/health.uconn.edu\/structural-biology\/protein-structures\/\">Back to Image Gallery<\/a><\/p>\n<\/div><\/div><\/div><\/div><\/div><\/div>","protected":false},"excerpt":{"rendered":"<p>Site-specific DNA recombination allows for controlled rearrangement of DNA segments to provide genetic diversity or a selective advantage for the organism. \u03b3\u03b4 resolvase is a site-specific DNA recombinase that catalyzes the final stage of the \u03b3\u03b4 DNA transposition in E. coli to form two catenated circular DNA products. \u03b3\u03b4 resolvase is a 20 kDa homodimer [&hellip;]<\/p>\n","protected":false},"author":38,"featured_media":0,"parent":155,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"acf":[],"publishpress_future_action":{"enabled":false,"date":"2026-05-01 14:38:10","action":"change-status","newStatus":"draft","terms":[],"taxonomy":""},"_links":{"self":[{"href":"https:\/\/health.uconn.edu\/structural-biology\/wp-json\/wp\/v2\/pages\/190"}],"collection":[{"href":"https:\/\/health.uconn.edu\/structural-biology\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/health.uconn.edu\/structural-biology\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/health.uconn.edu\/structural-biology\/wp-json\/wp\/v2\/users\/38"}],"replies":[{"embeddable":true,"href":"https:\/\/health.uconn.edu\/structural-biology\/wp-json\/wp\/v2\/comments?post=190"}],"version-history":[{"count":11,"href":"https:\/\/health.uconn.edu\/structural-biology\/wp-json\/wp\/v2\/pages\/190\/revisions"}],"predecessor-version":[{"id":537,"href":"https:\/\/health.uconn.edu\/structural-biology\/wp-json\/wp\/v2\/pages\/190\/revisions\/537"}],"up":[{"embeddable":true,"href":"https:\/\/health.uconn.edu\/structural-biology\/wp-json\/wp\/v2\/pages\/155"}],"wp:attachment":[{"href":"https:\/\/health.uconn.edu\/structural-biology\/wp-json\/wp\/v2\/media?parent=190"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}