{"id":183,"date":"2017-10-31T13:11:08","date_gmt":"2017-10-31T17:11:08","guid":{"rendered":"https:\/\/health.uconn.edu\/structural-biology\/?page_id=183"},"modified":"2017-11-03T15:39:29","modified_gmt":"2017-11-03T19:39:29","slug":"dna-polymerase-x","status":"publish","type":"page","link":"https:\/\/health.uconn.edu\/structural-biology\/protein-structures\/dna-polymerase-x\/","title":{"rendered":"DNA Polymerase X"},"content":{"rendered":"<div id=\"pl-183\"  class=\"panel-layout\" ><div id=\"pg-183-0\"  class=\"panel-grid panel-no-style\" ><div id=\"pgc-183-0-0\"  class=\"panel-grid-cell\" ><div id=\"panel-183-0-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"0\" ><div class=\"textwidget\"><p><div style=\"width: 400px;\" class=\"wp-video\"><!--[if lt IE 9]><script>document.createElement('video');<\/script><![endif]-->\n<video class=\"wp-video-shortcode\" id=\"video-183-1\" width=\"400\" height=\"360\" loop=\"1\" autoplay=\"1\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"https:\/\/health.uconn.edu\/structural-biology\/wp-content\/uploads\/sites\/177\/2017\/11\/Dna-Polymerase-X-1.mp4?_=1\" \/><a href=\"https:\/\/health.uconn.edu\/structural-biology\/wp-content\/uploads\/sites\/177\/2017\/11\/Dna-Polymerase-X-1.mp4\">https:\/\/health.uconn.edu\/structural-biology\/wp-content\/uploads\/sites\/177\/2017\/11\/Dna-Polymerase-X-1.mp4<\/a><\/video><\/div><\/p>\n<\/div><\/div><\/div><div id=\"pgc-183-0-1\"  class=\"panel-grid-cell\" ><div class=\"panel-cell-style panel-cell-style-for-183-0-1\" ><div id=\"panel-183-0-1-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"1\" ><div class=\"textwidget\"><p>DNA polymerase X (Pol X) from African swine fever virus is the smallest known nucleotide polymerase. It has extremely low fidelity and may act as a mutase to alter the viral genome. Although it lacks the canonical DNA binding domain of other nucleotide polymerases, Pol X binds DNA with very high affinity. It also bind dNTP substrates in the absence of template DNA. It has highest highest affinity for dGTP, which may contribute to its low fidelity and preference for making GG mismatches.<\/p>\n<hr \/>\n<p>Maciejewski, M.W., Shin, R., Pan, B., Marintchev, A., Denninger, A., Mullen, M.A., Kang, C., Gryk, M.R. and Mullen, G.P. (2001) Solution structure of a viral DNA repair polymerase.\u00a0<em>Nature Structural Biology<\/em>\u00a08, 936-941.\u00a0<\/p>\n<p><a href=\"http:\/\/www.ncbi.nlm.nih.gov\/entrez\/query.fcgi?cmd=Retrieve&amp;db=PubMed&amp;list_uids=11685238&amp;dopt=Abstract\">PubMed<\/a>\u00a0|\u00a0<a href=\"http:\/\/www.nature.com\/cgi-taf\/DynaPage.taf?file=\/nsb\/journal\/v8\/n11\/abs\/nsb1101-936.html&amp;dynoptions=doi1035509692\" class=\"broken_link\">Journal<\/a>\u00a0|\u00a0<a href=\"http:\/\/www.nature.com\/cgi-taf\/DynaPage.taf?file=\/nsb\/journal\/v8\/n11\/full\/nsb1101-915.html\" class=\"broken_link\">News &amp; Views<\/a>\u00a0|\u00a0<a href=\"http:\/\/www.rcsb.org\/pdb\/cgi\/explore.cgi?pid=21931037809366&amp;pdbId=1JAJ\" class=\"broken_link\">PDB Link<\/a><\/p>\n<p><a href=\"https:\/\/health.uconn.edu\/structural-biology\/protein-structures\/\">Back to Image Gallery<\/a><\/p>\n<\/div><\/div><\/div><\/div><\/div><\/div>","protected":false},"excerpt":{"rendered":"<p>DNA polymerase X (Pol X) from African swine fever virus is the smallest known nucleotide polymerase. It has extremely low fidelity and may act as a mutase to alter the viral genome. Although it lacks the canonical DNA binding domain of other nucleotide polymerases, Pol X binds DNA with very high affinity. It also bind [&hellip;]<\/p>\n","protected":false},"author":38,"featured_media":0,"parent":155,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"acf":[],"publishpress_future_action":{"enabled":false,"date":"2026-05-02 00:53:52","action":"change-status","newStatus":"draft","terms":[],"taxonomy":""},"_links":{"self":[{"href":"https:\/\/health.uconn.edu\/structural-biology\/wp-json\/wp\/v2\/pages\/183"}],"collection":[{"href":"https:\/\/health.uconn.edu\/structural-biology\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/health.uconn.edu\/structural-biology\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/health.uconn.edu\/structural-biology\/wp-json\/wp\/v2\/users\/38"}],"replies":[{"embeddable":true,"href":"https:\/\/health.uconn.edu\/structural-biology\/wp-json\/wp\/v2\/comments?post=183"}],"version-history":[{"count":14,"href":"https:\/\/health.uconn.edu\/structural-biology\/wp-json\/wp\/v2\/pages\/183\/revisions"}],"predecessor-version":[{"id":549,"href":"https:\/\/health.uconn.edu\/structural-biology\/wp-json\/wp\/v2\/pages\/183\/revisions\/549"}],"up":[{"embeddable":true,"href":"https:\/\/health.uconn.edu\/structural-biology\/wp-json\/wp\/v2\/pages\/155"}],"wp:attachment":[{"href":"https:\/\/health.uconn.edu\/structural-biology\/wp-json\/wp\/v2\/media?parent=183"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}