{"id":21,"date":"2018-03-10T13:14:49","date_gmt":"2018-03-10T18:14:49","guid":{"rendered":"https:\/\/health.uconn.edu\/pinter-lab\/?page_id=21"},"modified":"2021-05-07T12:15:48","modified_gmt":"2021-05-07T16:15:48","slug":"research","status":"publish","type":"page","link":"https:\/\/health.uconn.edu\/pinter-lab\/research\/","title":{"rendered":"Research"},"content":{"rendered":"<div id=\"pl-21\"  class=\"panel-layout\" ><div id=\"pg-21-0\"  class=\"panel-grid panel-no-style\" ><div id=\"pgc-21-0-0\"  class=\"panel-grid-cell\" ><div id=\"panel-21-0-0-0\" class=\"so-panel widget widget_metaslider_widget panel-first-child panel-last-child\" data-index=\"0\" ><div id=\"metaslider-id-92\" style=\"width: 100%; margin: 0 auto;\" class=\"ml-slider-3-80-0 ml-slider-pro-2-36-0 metaslider metaslider-flex metaslider-92 ml-slider ms-theme-outline nav-hidden\" role=\"region\" aria-roledescription=\"Slideshow\" aria-label=\"Cells\">\n    <div id=\"metaslider_container_92\">\n        <div id=\"metaslider_92\">\n            <ul class='slides'>\n                <li style=\"display: block; width: 100%;\" class=\"slide-157 ms-image \" aria-roledescription=\"slide\" aria-label=\"slide-157\"><img loading=\"lazy\" decoding=\"async\" data-src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2018\/08\/merged_green_SetMin_100_red_SetMax_100.tif-RGB-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"\" class=\"slider-92 slide-157 lazyload\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1200px; --smush-placeholder-aspect-ratio: 1200\/800;\" \/><noscript><img loading=\"lazy\" decoding=\"async\" data-src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2018\/08\/merged_green_SetMin_100_red_SetMax_100.tif-RGB-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"\" class=\"slider-92 slide-157 lazyload\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1200px; --smush-placeholder-aspect-ratio: 1200\/800;\" \/><noscript><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2018\/08\/merged_green_SetMin_100_red_SetMax_100.tif-RGB-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"\" class=\"slider-92 slide-157\" \/><\/noscript><\/noscript><\/li>\n                <li style=\"display: none; width: 100%;\" class=\"slide-154 ms-image \" aria-roledescription=\"slide\" aria-label=\"slide-154\"><img loading=\"lazy\" decoding=\"async\" data-src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2018\/08\/XIST488-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"\" class=\"slider-92 slide-154 lazyload\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1200px; --smush-placeholder-aspect-ratio: 1200\/800;\" \/><noscript><img loading=\"lazy\" decoding=\"async\" data-src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2018\/08\/XIST488-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"\" class=\"slider-92 slide-154 lazyload\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1200px; --smush-placeholder-aspect-ratio: 1200\/800;\" \/><noscript><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2018\/08\/XIST488-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"\" class=\"slider-92 slide-154\" \/><\/noscript><\/noscript><\/li>\n                <li style=\"display: none; width: 100%;\" class=\"slide-155 ms-image \" aria-roledescription=\"slide\" aria-label=\"slide-155\"><img loading=\"lazy\" decoding=\"async\" data-src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2018\/08\/FIRRE647-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"\" class=\"slider-92 slide-155 lazyload\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1200px; --smush-placeholder-aspect-ratio: 1200\/800;\" \/><noscript><img loading=\"lazy\" decoding=\"async\" data-src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2018\/08\/FIRRE647-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"\" class=\"slider-92 slide-155 lazyload\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1200px; --smush-placeholder-aspect-ratio: 1200\/800;\" \/><noscript><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2018\/08\/FIRRE647-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"\" class=\"slider-92 slide-155\" \/><\/noscript><\/noscript><\/li>\n                <li style=\"display: none; width: 100%;\" class=\"slide-156 ms-image \" aria-roledescription=\"slide\" aria-label=\"slide-156\"><img loading=\"lazy\" decoding=\"async\" data-src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2018\/08\/ATPASE555-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"\" class=\"slider-92 slide-156 lazyload\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1200px; --smush-placeholder-aspect-ratio: 1200\/800;\" \/><noscript><img loading=\"lazy\" decoding=\"async\" data-src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2018\/08\/ATPASE555-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"\" class=\"slider-92 slide-156 lazyload\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1200px; --smush-placeholder-aspect-ratio: 1200\/800;\" \/><noscript><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2018\/08\/ATPASE555-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"\" class=\"slider-92 slide-156\" \/><\/noscript><\/noscript><\/li>\n                <li style=\"display: none; width: 100%;\" class=\"slide-434 ms-image \" aria-roledescription=\"slide\" aria-label=\"slide-434\"><img loading=\"lazy\" decoding=\"async\" data-src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2020\/04\/198-1-w1-Cy5-3_Plate_D_p00_0_A01f02d3-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"An immunofluorescent image of 14 week old neurons.\" class=\"slider-92 slide-434 lazyload\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1200px; --smush-placeholder-aspect-ratio: 1200\/800;\" \/><noscript><img loading=\"lazy\" decoding=\"async\" data-src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2020\/04\/198-1-w1-Cy5-3_Plate_D_p00_0_A01f02d3-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"An immunofluorescent image of 14 week old neurons.\" class=\"slider-92 slide-434 lazyload\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1200px; --smush-placeholder-aspect-ratio: 1200\/800;\" \/><noscript><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2020\/04\/198-1-w1-Cy5-3_Plate_D_p00_0_A01f02d3-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"An immunofluorescent image of 14 week old neurons.\" class=\"slider-92 slide-434\" \/><\/noscript><\/noscript><\/li>\n                <li style=\"display: none; width: 100%;\" class=\"slide-435 ms-image \" aria-roledescription=\"slide\" aria-label=\"slide-435\"><img loading=\"lazy\" decoding=\"async\" data-src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2020\/04\/198-5-w1-cy5-2_Plate_D_p00_0_C01f01d3-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"An immunofluorescent image of 14 week old neurons.\" class=\"slider-92 slide-435 lazyload\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1200px; --smush-placeholder-aspect-ratio: 1200\/800;\" \/><noscript><img loading=\"lazy\" decoding=\"async\" data-src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2020\/04\/198-5-w1-cy5-2_Plate_D_p00_0_C01f01d3-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"An immunofluorescent image of 14 week old neurons.\" class=\"slider-92 slide-435 lazyload\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1200px; --smush-placeholder-aspect-ratio: 1200\/800;\" \/><noscript><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2020\/04\/198-5-w1-cy5-2_Plate_D_p00_0_C01f01d3-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"An immunofluorescent image of 14 week old neurons.\" class=\"slider-92 slide-435\" \/><\/noscript><\/noscript><\/li>\n                <li style=\"display: none; width: 100%;\" class=\"slide-436 ms-image \" aria-roledescription=\"slide\" aria-label=\"slide-436\"><img loading=\"lazy\" decoding=\"async\" data-src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2020\/04\/DIG-EVEN-40x-iPSC-merge-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"An image of cells with Xist clouds in iPSCs, identified by fluorescent in situ hybridization.\" class=\"slider-92 slide-436 lazyload\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1200px; --smush-placeholder-aspect-ratio: 1200\/800;\" \/><noscript><img loading=\"lazy\" decoding=\"async\" data-src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2020\/04\/DIG-EVEN-40x-iPSC-merge-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"An image of cells with Xist clouds in iPSCs, identified by fluorescent in situ hybridization.\" class=\"slider-92 slide-436 lazyload\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1200px; --smush-placeholder-aspect-ratio: 1200\/800;\" \/><noscript><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2020\/04\/DIG-EVEN-40x-iPSC-merge-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"An image of cells with Xist clouds in iPSCs, identified by fluorescent in situ hybridization.\" class=\"slider-92 slide-436\" \/><\/noscript><\/noscript><\/li>\n                <li style=\"display: none; width: 100%;\" class=\"slide-530 ms-image \" aria-roledescription=\"slide\" aria-label=\"slide-530\"><img loading=\"lazy\" decoding=\"async\" data-src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2020\/05\/C6XX_Diff51_bottom-1200x800.png\" height=\"800\" width=\"1200\" alt=\"An immunofluorescence image of iPSC-derived trophoblasts.\" class=\"slider-92 slide-530 lazyload\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1200px; --smush-placeholder-aspect-ratio: 1200\/800;\" \/><noscript><img loading=\"lazy\" decoding=\"async\" data-src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2020\/05\/C6XX_Diff51_bottom-1200x800.png\" height=\"800\" width=\"1200\" alt=\"An immunofluorescence image of iPSC-derived trophoblasts.\" class=\"slider-92 slide-530 lazyload\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1200px; --smush-placeholder-aspect-ratio: 1200\/800;\" \/><noscript><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2020\/05\/C6XX_Diff51_bottom-1200x800.png\" height=\"800\" width=\"1200\" alt=\"An immunofluorescence image of iPSC-derived trophoblasts.\" class=\"slider-92 slide-530\" \/><\/noscript><\/noscript><\/li>\n                <li style=\"display: none; width: 100%;\" class=\"slide-531 ms-image \" aria-roledescription=\"slide\" aria-label=\"slide-531\"><img loading=\"lazy\" decoding=\"async\" data-src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2020\/05\/NCC25_C23XX_D5-1200x800.png\" height=\"800\" width=\"1200\" alt=\"An immunofluorescence image of iPSC-derived neural crest cells.\" class=\"slider-92 slide-531 lazyload\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1200px; --smush-placeholder-aspect-ratio: 1200\/800;\" \/><noscript><img loading=\"lazy\" decoding=\"async\" data-src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2020\/05\/NCC25_C23XX_D5-1200x800.png\" height=\"800\" width=\"1200\" alt=\"An immunofluorescence image of iPSC-derived neural crest cells.\" class=\"slider-92 slide-531 lazyload\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1200px; --smush-placeholder-aspect-ratio: 1200\/800;\" \/><noscript><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2020\/05\/NCC25_C23XX_D5-1200x800.png\" height=\"800\" width=\"1200\" alt=\"An immunofluorescence image of iPSC-derived neural crest cells.\" class=\"slider-92 slide-531\" \/><\/noscript><\/noscript><\/li>\n                <li style=\"display: none; width: 100%;\" class=\"slide-532 ms-image \" aria-roledescription=\"slide\" aria-label=\"slide-532\"><img loading=\"lazy\" decoding=\"async\" data-src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2020\/05\/FISH_C6XXp23Gel-1200x800.png\" height=\"800\" width=\"1200\" alt=\"An image of cells with Xist clouds identified by fluorescent in situ hybridization.\" class=\"slider-92 slide-532 lazyload\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1200px; --smush-placeholder-aspect-ratio: 1200\/800;\" \/><noscript><img loading=\"lazy\" decoding=\"async\" data-src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2020\/05\/FISH_C6XXp23Gel-1200x800.png\" height=\"800\" width=\"1200\" alt=\"An image of cells with Xist clouds identified by fluorescent in situ hybridization.\" class=\"slider-92 slide-532 lazyload\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1200px; --smush-placeholder-aspect-ratio: 1200\/800;\" \/><noscript><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2020\/05\/FISH_C6XXp23Gel-1200x800.png\" height=\"800\" width=\"1200\" alt=\"An image of cells with Xist clouds identified by fluorescent in situ hybridization.\" class=\"slider-92 slide-532\" \/><\/noscript><\/noscript><\/li>\n                <li style=\"display: none; width: 100%;\" class=\"slide-550 ms-image \" aria-roledescription=\"slide\" aria-label=\"slide-550\"><img loading=\"lazy\" decoding=\"async\" data-src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2020\/05\/CS-23_ch2-20x-1a_composite-copy-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"An immunofluorescence image of iPSC-derived smooth muscle cells.\" class=\"slider-92 slide-550 lazyload\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1200px; --smush-placeholder-aspect-ratio: 1200\/800;\" \/><noscript><img loading=\"lazy\" decoding=\"async\" data-src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2020\/05\/CS-23_ch2-20x-1a_composite-copy-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"An immunofluorescence image of iPSC-derived smooth muscle cells.\" class=\"slider-92 slide-550 lazyload\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1200px; --smush-placeholder-aspect-ratio: 1200\/800;\" \/><noscript><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2020\/05\/CS-23_ch2-20x-1a_composite-copy-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"An immunofluorescence image of iPSC-derived smooth muscle cells.\" class=\"slider-92 slide-550\" \/><\/noscript><\/noscript><\/li>\n            <\/ul>\n        <\/div>\n        \n    <\/div>\n<\/div><\/div><\/div><div id=\"pgc-21-0-1\"  class=\"panel-grid-cell\" ><div id=\"panel-21-0-1-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"1\" ><div class=\"textwidget\"><p style=\"text-align: left;\"><strong>Our primary research interest is to understand how chromosomal gene dosage impacts human development, specifically in two viable human aneuploidy conditions: Turner Syndrome (monosomy X) and Down syndrome (trisomy 21). To this end, we develop computational &amp; molecular approaches to accurately distinguish parental alleles, and alter their expression across chromosomes X &amp; 21 using long non-coding RNA and CRISPR-mounted tools. We apply these techniques in human induced pluripotent stem cells (hiPSCs) that we derived alongside isogenic euploid control lines to model developmental aspects of Turner &amp; Down syndrome <em>in vitro<\/em>.<\/strong><\/p>\n<p style=\"text-align: left;\">The mammalian X provides a unique perspective on chromosomal gene dosage, as a classic model of epigenetics: X chromosome inactivation (XCI), the process by which one X chromosome in females is silenced to achieve gene dosage parity with males. To establish XCI, the long non-coding RNA <em>XIST<\/em> accumulates on the X from which it is transcribed, and orchestrates a series of changes in subnuclear localization, chromosome topology, chromatin compaction and regulatory modifications to stably silence genes in <em>cis. <\/em>Some genes however, are skipped by <em>XIST<\/em> and remain active on the inactive X chromosome, thereby escaping XCI. Our long-term goal is understand the mechanisms that insulate such \u201cescapee\u201d genes against the spread of heterochromatin, and translate these lessons to X-linked genetic disorders that can manifest heterozygous females, including Rett\u2019s syndrome and Duchenne\u2019s muscular dystrophy.<\/p>\n<\/div><\/div><\/div><\/div><div id=\"pg-21-1\"  class=\"panel-grid panel-no-style\" ><div id=\"pgc-21-1-0\"  class=\"panel-grid-cell\" ><div id=\"panel-21-1-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"2\" ><div class=\"textwidget\"><p>Importantly, because many escapee genes have Y-linked homologs (X-Y pair or gametologs), they are expressed from two active copies in both XY males and XX females. Turner\u2019s syndrome (TS, karyotype 45,X) is hypothesized to result from haploinsufficiency in some of these genes, but only its cardinal phenotype (short stature) has been conclusively mapped to an X-Y pair to-date. Yet, by far the most frequent outcome of XO karyotype, is failure to reach term (est. 10-15% of all spontaneous terminations), and life-span in TS patients is reduced (~15 years) due to progressive aortic defects. We aim to use our TS hiPSC models to determine which genes contribute to the (extra)-embryonic defects that underpin these TS phenotypes.<\/p>\n<p>In contrast, excess gene dosage from chromosome 21 causes Down Syndrome (DS), the most frequent viable aneuploidy in humans (karyotype 47, +21 incidence of 1 in 700). DS encompasses a range of phenotypes including congenital heart disease, immunodeficiency, and intellectual disability. In concert with signs of accelerated aging, middle-aged individuals with DS tend to develop highly-penetrant, early-onset Alzheimer\u2019s disease (AD), making DS the most frequent single genetic cause of AD overall. We are re-purposing XIST to silence one of the three copies of chromosome 21 in our DS hiPSCs learn how trisomy 21 impacts neural development. Using allele-specific CRISPR-mounted tools, we also aim to dissect which genes contribute to DS-relevant cellular phenotypes and to explore avenues to correct their dosage.<\/p>\n<\/div><\/div><\/div><div id=\"pgc-21-1-1\"  class=\"panel-grid-cell\" ><div id=\"panel-21-1-1-0\" class=\"so-panel widget widget_metaslider_widget panel-first-child panel-last-child\" data-index=\"3\" ><div id=\"metaslider-id-92\" style=\"width: 100%; margin: 0 auto;\" class=\"ml-slider-3-80-0 ml-slider-pro-2-36-0 metaslider metaslider-flex metaslider-92 ml-slider ms-theme-outline nav-hidden nav-hidden\" role=\"region\" aria-roledescription=\"Slideshow\" aria-label=\"Cells\">\n    <div id=\"metaslider_container_92\">\n        <div id=\"metaslider_92\">\n            <ul class='slides'>\n                <li style=\"display: block; width: 100%;\" class=\"slide-157 ms-image \" aria-roledescription=\"slide\" aria-label=\"slide-157\"><img loading=\"lazy\" decoding=\"async\" data-src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2018\/08\/merged_green_SetMin_100_red_SetMax_100.tif-RGB-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"\" class=\"slider-92 slide-157 lazyload\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1200px; --smush-placeholder-aspect-ratio: 1200\/800;\" \/><noscript><img loading=\"lazy\" decoding=\"async\" data-src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2018\/08\/merged_green_SetMin_100_red_SetMax_100.tif-RGB-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"\" class=\"slider-92 slide-157 lazyload\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1200px; --smush-placeholder-aspect-ratio: 1200\/800;\" \/><noscript><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2018\/08\/merged_green_SetMin_100_red_SetMax_100.tif-RGB-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"\" class=\"slider-92 slide-157\" \/><\/noscript><\/noscript><\/li>\n                <li style=\"display: none; width: 100%;\" class=\"slide-154 ms-image \" aria-roledescription=\"slide\" aria-label=\"slide-154\"><img loading=\"lazy\" decoding=\"async\" data-src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2018\/08\/XIST488-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"\" class=\"slider-92 slide-154 lazyload\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1200px; --smush-placeholder-aspect-ratio: 1200\/800;\" \/><noscript><img loading=\"lazy\" decoding=\"async\" data-src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2018\/08\/XIST488-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"\" class=\"slider-92 slide-154 lazyload\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1200px; --smush-placeholder-aspect-ratio: 1200\/800;\" \/><noscript><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2018\/08\/XIST488-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"\" class=\"slider-92 slide-154\" \/><\/noscript><\/noscript><\/li>\n                <li style=\"display: none; width: 100%;\" class=\"slide-155 ms-image \" aria-roledescription=\"slide\" aria-label=\"slide-155\"><img loading=\"lazy\" decoding=\"async\" data-src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2018\/08\/FIRRE647-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"\" class=\"slider-92 slide-155 lazyload\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1200px; --smush-placeholder-aspect-ratio: 1200\/800;\" \/><noscript><img loading=\"lazy\" decoding=\"async\" data-src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2018\/08\/FIRRE647-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"\" class=\"slider-92 slide-155 lazyload\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1200px; --smush-placeholder-aspect-ratio: 1200\/800;\" \/><noscript><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2018\/08\/FIRRE647-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"\" class=\"slider-92 slide-155\" \/><\/noscript><\/noscript><\/li>\n                <li style=\"display: none; width: 100%;\" class=\"slide-156 ms-image \" aria-roledescription=\"slide\" aria-label=\"slide-156\"><img loading=\"lazy\" decoding=\"async\" data-src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2018\/08\/ATPASE555-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"\" class=\"slider-92 slide-156 lazyload\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1200px; --smush-placeholder-aspect-ratio: 1200\/800;\" \/><noscript><img loading=\"lazy\" decoding=\"async\" data-src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2018\/08\/ATPASE555-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"\" class=\"slider-92 slide-156 lazyload\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1200px; --smush-placeholder-aspect-ratio: 1200\/800;\" \/><noscript><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2018\/08\/ATPASE555-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"\" class=\"slider-92 slide-156\" \/><\/noscript><\/noscript><\/li>\n                <li style=\"display: none; width: 100%;\" class=\"slide-434 ms-image \" aria-roledescription=\"slide\" aria-label=\"slide-434\"><img loading=\"lazy\" decoding=\"async\" data-src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2020\/04\/198-1-w1-Cy5-3_Plate_D_p00_0_A01f02d3-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"An immunofluorescent image of 14 week old neurons.\" class=\"slider-92 slide-434 lazyload\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1200px; --smush-placeholder-aspect-ratio: 1200\/800;\" \/><noscript><img loading=\"lazy\" decoding=\"async\" data-src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2020\/04\/198-1-w1-Cy5-3_Plate_D_p00_0_A01f02d3-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"An immunofluorescent image of 14 week old neurons.\" class=\"slider-92 slide-434 lazyload\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1200px; --smush-placeholder-aspect-ratio: 1200\/800;\" \/><noscript><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2020\/04\/198-1-w1-Cy5-3_Plate_D_p00_0_A01f02d3-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"An immunofluorescent image of 14 week old neurons.\" class=\"slider-92 slide-434\" \/><\/noscript><\/noscript><\/li>\n                <li style=\"display: none; width: 100%;\" class=\"slide-435 ms-image \" aria-roledescription=\"slide\" aria-label=\"slide-435\"><img loading=\"lazy\" decoding=\"async\" data-src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2020\/04\/198-5-w1-cy5-2_Plate_D_p00_0_C01f01d3-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"An immunofluorescent image of 14 week old neurons.\" class=\"slider-92 slide-435 lazyload\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1200px; --smush-placeholder-aspect-ratio: 1200\/800;\" \/><noscript><img loading=\"lazy\" decoding=\"async\" data-src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2020\/04\/198-5-w1-cy5-2_Plate_D_p00_0_C01f01d3-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"An immunofluorescent image of 14 week old neurons.\" class=\"slider-92 slide-435 lazyload\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1200px; --smush-placeholder-aspect-ratio: 1200\/800;\" \/><noscript><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2020\/04\/198-5-w1-cy5-2_Plate_D_p00_0_C01f01d3-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"An immunofluorescent image of 14 week old neurons.\" class=\"slider-92 slide-435\" \/><\/noscript><\/noscript><\/li>\n                <li style=\"display: none; width: 100%;\" class=\"slide-436 ms-image \" aria-roledescription=\"slide\" aria-label=\"slide-436\"><img loading=\"lazy\" decoding=\"async\" data-src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2020\/04\/DIG-EVEN-40x-iPSC-merge-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"An image of cells with Xist clouds in iPSCs, identified by fluorescent in situ hybridization.\" class=\"slider-92 slide-436 lazyload\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1200px; --smush-placeholder-aspect-ratio: 1200\/800;\" \/><noscript><img loading=\"lazy\" decoding=\"async\" data-src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2020\/04\/DIG-EVEN-40x-iPSC-merge-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"An image of cells with Xist clouds in iPSCs, identified by fluorescent in situ hybridization.\" class=\"slider-92 slide-436 lazyload\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1200px; --smush-placeholder-aspect-ratio: 1200\/800;\" \/><noscript><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2020\/04\/DIG-EVEN-40x-iPSC-merge-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"An image of cells with Xist clouds in iPSCs, identified by fluorescent in situ hybridization.\" class=\"slider-92 slide-436\" \/><\/noscript><\/noscript><\/li>\n                <li style=\"display: none; width: 100%;\" class=\"slide-530 ms-image \" aria-roledescription=\"slide\" aria-label=\"slide-530\"><img loading=\"lazy\" decoding=\"async\" data-src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2020\/05\/C6XX_Diff51_bottom-1200x800.png\" height=\"800\" width=\"1200\" alt=\"An immunofluorescence image of iPSC-derived trophoblasts.\" class=\"slider-92 slide-530 lazyload\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1200px; --smush-placeholder-aspect-ratio: 1200\/800;\" \/><noscript><img loading=\"lazy\" decoding=\"async\" data-src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2020\/05\/C6XX_Diff51_bottom-1200x800.png\" height=\"800\" width=\"1200\" alt=\"An immunofluorescence image of iPSC-derived trophoblasts.\" class=\"slider-92 slide-530 lazyload\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1200px; --smush-placeholder-aspect-ratio: 1200\/800;\" \/><noscript><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2020\/05\/C6XX_Diff51_bottom-1200x800.png\" height=\"800\" width=\"1200\" alt=\"An immunofluorescence image of iPSC-derived trophoblasts.\" class=\"slider-92 slide-530\" \/><\/noscript><\/noscript><\/li>\n                <li style=\"display: none; width: 100%;\" class=\"slide-531 ms-image \" aria-roledescription=\"slide\" aria-label=\"slide-531\"><img loading=\"lazy\" decoding=\"async\" data-src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2020\/05\/NCC25_C23XX_D5-1200x800.png\" height=\"800\" width=\"1200\" alt=\"An immunofluorescence image of iPSC-derived neural crest cells.\" class=\"slider-92 slide-531 lazyload\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1200px; --smush-placeholder-aspect-ratio: 1200\/800;\" \/><noscript><img loading=\"lazy\" decoding=\"async\" data-src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2020\/05\/NCC25_C23XX_D5-1200x800.png\" height=\"800\" width=\"1200\" alt=\"An immunofluorescence image of iPSC-derived neural crest cells.\" class=\"slider-92 slide-531 lazyload\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1200px; --smush-placeholder-aspect-ratio: 1200\/800;\" \/><noscript><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2020\/05\/NCC25_C23XX_D5-1200x800.png\" height=\"800\" width=\"1200\" alt=\"An immunofluorescence image of iPSC-derived neural crest cells.\" class=\"slider-92 slide-531\" \/><\/noscript><\/noscript><\/li>\n                <li style=\"display: none; width: 100%;\" class=\"slide-532 ms-image \" aria-roledescription=\"slide\" aria-label=\"slide-532\"><img loading=\"lazy\" decoding=\"async\" data-src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2020\/05\/FISH_C6XXp23Gel-1200x800.png\" height=\"800\" width=\"1200\" alt=\"An image of cells with Xist clouds identified by fluorescent in situ hybridization.\" class=\"slider-92 slide-532 lazyload\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1200px; --smush-placeholder-aspect-ratio: 1200\/800;\" \/><noscript><img loading=\"lazy\" decoding=\"async\" data-src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2020\/05\/FISH_C6XXp23Gel-1200x800.png\" height=\"800\" width=\"1200\" alt=\"An image of cells with Xist clouds identified by fluorescent in situ hybridization.\" class=\"slider-92 slide-532 lazyload\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1200px; --smush-placeholder-aspect-ratio: 1200\/800;\" \/><noscript><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2020\/05\/FISH_C6XXp23Gel-1200x800.png\" height=\"800\" width=\"1200\" alt=\"An image of cells with Xist clouds identified by fluorescent in situ hybridization.\" class=\"slider-92 slide-532\" \/><\/noscript><\/noscript><\/li>\n                <li style=\"display: none; width: 100%;\" class=\"slide-550 ms-image \" aria-roledescription=\"slide\" aria-label=\"slide-550\"><img loading=\"lazy\" decoding=\"async\" data-src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2020\/05\/CS-23_ch2-20x-1a_composite-copy-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"An immunofluorescence image of iPSC-derived smooth muscle cells.\" class=\"slider-92 slide-550 lazyload\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1200px; --smush-placeholder-aspect-ratio: 1200\/800;\" \/><noscript><img loading=\"lazy\" decoding=\"async\" data-src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2020\/05\/CS-23_ch2-20x-1a_composite-copy-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"An immunofluorescence image of iPSC-derived smooth muscle cells.\" class=\"slider-92 slide-550 lazyload\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1200px; --smush-placeholder-aspect-ratio: 1200\/800;\" \/><noscript><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/health.uconn.edu\/pinter-lab\/wp-content\/uploads\/sites\/92\/2020\/05\/CS-23_ch2-20x-1a_composite-copy-1200x800.jpg\" height=\"800\" width=\"1200\" alt=\"An immunofluorescence image of iPSC-derived smooth muscle cells.\" class=\"slider-92 slide-550\" \/><\/noscript><\/noscript><\/li>\n            <\/ul>\n        <\/div>\n        \n    <\/div>\n<\/div><\/div><\/div><\/div><\/div>","protected":false},"excerpt":{"rendered":"<p>Our primary research interest is to understand how chromosomal gene dosage impacts human development, specifically in two viable human aneuploidy conditions: Turner Syndrome (monosomy X) and Down syndrome (trisomy 21). To this end, we develop computational &amp; molecular approaches to accurately distinguish parental alleles, and alter their expression across chromosomes X &amp; 21 using long [&hellip;]<\/p>\n","protected":false},"author":336,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"acf":[],"publishpress_future_action":{"enabled":false,"date":"2026-04-30 18:57:35","action":"change-status","newStatus":"draft","terms":[],"taxonomy":""},"_links":{"self":[{"href":"https:\/\/health.uconn.edu\/pinter-lab\/wp-json\/wp\/v2\/pages\/21"}],"collection":[{"href":"https:\/\/health.uconn.edu\/pinter-lab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/health.uconn.edu\/pinter-lab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/health.uconn.edu\/pinter-lab\/wp-json\/wp\/v2\/users\/336"}],"replies":[{"embeddable":true,"href":"https:\/\/health.uconn.edu\/pinter-lab\/wp-json\/wp\/v2\/comments?post=21"}],"version-history":[{"count":41,"href":"https:\/\/health.uconn.edu\/pinter-lab\/wp-json\/wp\/v2\/pages\/21\/revisions"}],"predecessor-version":[{"id":580,"href":"https:\/\/health.uconn.edu\/pinter-lab\/wp-json\/wp\/v2\/pages\/21\/revisions\/580"}],"wp:attachment":[{"href":"https:\/\/health.uconn.edu\/pinter-lab\/wp-json\/wp\/v2\/media?parent=21"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}