{"id":153,"date":"2017-07-21T13:25:52","date_gmt":"2017-07-21T17:25:52","guid":{"rendered":"https:\/\/health.uconn.edu\/neuropeptide-lab\/?page_id=153"},"modified":"2017-07-24T10:11:16","modified_gmt":"2017-07-24T14:11:16","slug":"neuropeptide-lab-antibodies-available","status":"publish","type":"page","link":"https:\/\/health.uconn.edu\/neuropeptide-lab\/neuropeptide-lab-antibodies-available\/","title":{"rendered":"Neuropeptide Lab Antibodies Available"},"content":{"rendered":"

All are rabbit polyclonals; performance of many is improved by affinity-purification.<\/p>\n

Requesting Neuropeptide Scientific Materials<\/a><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Antigen<\/strong><\/td>\nIdentification<\/strong><\/td>\nUsage<\/strong><\/td>\nAffinity-purification<\/strong><\/td>\nReference<\/strong><\/td>\n<\/tr>\n
Peptides<\/strong><\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
ACTH(18-39)<\/td>\nJH44<\/td>\nRIA; Westerns; immunostain<\/td>\nno<\/td>\nSchnabel et al 1989 Mol Endo 3:1223<\/td>\n<\/tr>\n
ACTH(18-39)<\/td>\nKathy<\/td>\nRIA; immunostain<\/td>\nno<\/td>\nMains&Eipper 1983 Endocrinology 112:1986<\/td>\n<\/tr>\n
g3<\/sub>MSH<\/td>\nJH189<\/td>\nWesterns<\/td>\nyes<\/td>\nNoel et al 1991 Mol Endo 5:404<\/td>\n<\/tr>\n
POMC N-term<\/td>\nGeorgie<\/td>\nWesterns, immunostaining<\/td>\nno; use 5% milk in Westerns<\/td>\nMains&Eipper 1979 J Biol Chem 254:7885<\/td>\n<\/tr>\n
NPY<\/td>\nJH3<\/td>\nImmunostaining<\/td>\nno<\/td>\nDickerson et al 1987 J Biol Chem 262:13646<\/td>\n<\/tr>\n
PAM<\/strong><\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
Rat PHM<\/td>\nJH1761<\/td>\nWesterns<\/td>\nyes<\/td>\nOyarce et al 2001 J Biol Chem 276:33265<\/td>\n<\/tr>\n
Rat PHM<\/td>\nJH475<\/td>\nWesterns, IPT<\/td>\nno<\/td>\nYun et al Arch Biochem Biophys 1993 301:77<\/td>\n<\/tr>\n
rPAM(116-131)<\/td>\nJH246<\/td>\nWestern<\/td>\nyes<\/td>\nEipper et al 1991 J Biol Chem 266:7827<\/td>\n<\/tr>\n
bPAM(288-310)<\/td>\nJH100<\/td>\nWestern<\/td>\nyes<\/td>\nHusten&Eipper 1991 J Biol Chem 266:17004<\/td>\n<\/tr>\n
rPAM exon 16<\/td>\nJH629<\/td>\nWestern, IPT, immunostaining<\/td>\nyes<\/td>\nMaltese&Eipper 1992 Mol Endo 6:1998<\/td>\n<\/tr>\n
bPAM(561-579)<\/td>\nJH69<\/td>\nWesterns<\/td>\nyes<\/td>\nHusten&Eipper 1991 J Biol Chem 266:17004<\/td>\n<\/tr>\n
rPAL<\/td>\nJH471<\/td>\nWestern<\/td>\nno<\/td>\nYun et al Arch Biochem Biophys 1993 301:77<\/td>\n<\/tr>\n
rPAL<\/td>\nJH877<\/td>\nWestern<\/td>\nno<\/td>\nKolhekar et al 2002 Biochemistry 41:12384<\/td>\n<\/tr>\n
rPAM C-term<\/td>\nCT267,268<\/td>\nWestern, IPT<\/td>\nyes<\/td>\nFrancone et al 2010 Mol Endo 24:1543<\/td>\n<\/tr>\n
rPAM-(p)S937<\/td>\nJH1922<\/td>\nWestern<\/td>\n<\/td>\nSteveson et al 1999 J Biol Chem 274:21128<\/td>\n<\/tr>\n
rPAM-CKII site<\/td>\nJH2540-42<\/td>\nWestern<\/td>\n<\/td>\nSteveson et al 2001 J Biol Chem 276:40326<\/td>\n<\/tr>\n
rPAM C-term<\/td>\n6E6\u00a0 (mAb)<\/td>\nWestern, Immunostain<\/td>\nNo need<\/td>\nMilgram et al 1997 J Cell Sci 110:695<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n
\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Kalirin<\/strong><\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
Kalirin, SR4-7<\/td>\nJH2580\/1\/2<\/td>\nWestern, IPT<\/td>\nyes<\/td>\nPenzes et al 2000 J Biol Chem 275:7395<\/td>\n<\/tr>\n
Kalirin, Sec14<\/td>\nCT301\/302<\/td>\nWestern, IPT<\/td>\nyes<\/td>\nMiller et al 2015 J Biol Chem 290:13541<\/td>\n<\/tr>\n
Kalirin-7 C-term<\/td>\nJH2958\/9<\/td>\nWestern, IPT, immunostaining<\/td>\nNo; very inefficient<\/td>\nPenzes et al 2000 J Biol Chem 275:7395<\/td>\n<\/tr>\n
Kalirin-12<\/td>\nJH3225\/7<\/td>\n<\/td>\n<\/td>\nJohnson et al 2000 J Biol Chem 275:19324<\/td>\n<\/tr>\n
Trio<\/strong><\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
Trio SR5<\/td>\nCT232\/233<\/td>\n<\/td>\n<\/td>\nMcPherson et al 2005 Gene 347:125<\/td>\n<\/tr>\n
hTrio Sec14<\/td>\nCT320\/21\/22<\/td>\n<\/td>\n<\/td>\nKatrancha et al submitted<\/td>\n<\/tr>\n
hTrio IgFnIII<\/td>\nCT217, 219<\/td>\n<\/td>\n<\/td>\nKatrancha et al submitted<\/td>\n<\/tr>\n
Others<\/strong><\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
Atp7a<\/td>\nCT77\/78<\/td>\nWestern, immunostaining<\/td>\nyes<\/td>\nSteveson et al 2003 Endocrinology 144:188<\/td>\n<\/tr>\n
RESP18<\/td>\nJH1162\/3<\/td>\nWestern, immunostaining<\/td>\nno<\/td>\nSchiller et al 1995 J Biol Chem 270:26129<\/td>\n<\/tr>\n
rDBM<\/td>\nJH3125\/6<\/td>\nWestern<\/td>\nno<\/td>\nXin et al 2004 J Biol Chem 279:48159<\/td>\n<\/tr>\n
PC1<\/td>\nJH888<\/td>\nWestern, immunostaining<\/td>\nyes<\/td>\nZhou&Mains 1994 J Biol Chem 269:17440<\/td>\n<\/tr>\n
PC2<\/td>\nJH1125\/6<\/td>\nWestern<\/td>\nyes<\/td>\nZhou&Mains 1994 J Biol Chem 269:17440<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

All are rabbit polyclonals; performance of many is improved by affinity-purification. Requesting Neuropeptide Scientific Materials Antigen Identification Usage Affinity-purification Reference Peptides ACTH(18-39) JH44 RIA; Westerns; immunostain no Schnabel et al 1989 Mol Endo 3:1223 ACTH(18-39) Kathy RIA; immunostain no Mains&Eipper 1983 Endocrinology 112:1986 g3MSH JH189 Westerns yes Noel et al 1991 Mol Endo 5:404 POMC […]<\/p>\n","protected":false},"author":97,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"acf":[],"publishpress_future_action":{"enabled":false,"date":"2026-04-27 16:22:34","action":"change-status","newStatus":"draft","terms":[],"taxonomy":""},"_links":{"self":[{"href":"https:\/\/health.uconn.edu\/neuropeptide-lab\/wp-json\/wp\/v2\/pages\/153"}],"collection":[{"href":"https:\/\/health.uconn.edu\/neuropeptide-lab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/health.uconn.edu\/neuropeptide-lab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/health.uconn.edu\/neuropeptide-lab\/wp-json\/wp\/v2\/users\/97"}],"replies":[{"embeddable":true,"href":"https:\/\/health.uconn.edu\/neuropeptide-lab\/wp-json\/wp\/v2\/comments?post=153"}],"version-history":[{"count":15,"href":"https:\/\/health.uconn.edu\/neuropeptide-lab\/wp-json\/wp\/v2\/pages\/153\/revisions"}],"predecessor-version":[{"id":186,"href":"https:\/\/health.uconn.edu\/neuropeptide-lab\/wp-json\/wp\/v2\/pages\/153\/revisions\/186"}],"wp:attachment":[{"href":"https:\/\/health.uconn.edu\/neuropeptide-lab\/wp-json\/wp\/v2\/media?parent=153"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}