{"id":991,"date":"2022-02-02T10:43:16","date_gmt":"2022-02-02T15:43:16","guid":{"rendered":"https:\/\/health.uconn.edu\/mouse-genome-modification\/?p=991"},"modified":"2025-07-01T08:54:43","modified_gmt":"2025-07-01T12:54:43","slug":"piggybac-on-bac","status":"publish","type":"post","link":"https:\/\/health.uconn.edu\/mouse-genome-modification\/2022\/02\/02\/piggybac-on-bac\/","title":{"rendered":"Piggy Bac Mediated Transgenesis"},"content":{"rendered":"<div id=\"pl-991\"  class=\"panel-layout\" ><div id=\"pg-991-0\"  class=\"panel-grid panel-no-style\" ><div id=\"pgc-991-0-0\"  class=\"panel-grid-cell\" ><div id=\"panel-991-0-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child\" data-index=\"0\" ><div class=\"textwidget\"><h2>New Services<\/h2>\n<p>Piggy Bac-mediated transgenesis offers many advantages over traditional transgenics. It has a large cargo capacity (up to 200 kb), allowing insertion of large promoters and regulatory elements. The insertion is footprint-free and does not leave behind vector sequences. The transgene is also inserted in the host genome in a precise manner as a single copy without any chromosomal rearrangement or deletion (chromothripsis) and therefore offers reliable and consistent transgene expression.<\/p>\n<p>In the Piggy Bac approach, the donor template includes the transgene of interest flanked by inverted terminal repeat (ITR) sequences. The donor template is microinjected into 1-cell embryos along with mRNA encoding the transposase, which integrates the transgene into the host genome through a \u201ccut and paste\u201d mechanism.<\/p>\n<p>The CMGM has generated several novel mouse models using this approach including cell specific ablation and conditional rescue humanized mouse models.<\/p>\n<\/div><\/div><div id=\"panel-991-0-0-1\" class=\"so-panel widget widget_widget_sp_image widget_sp_image panel-last-child\" data-index=\"1\" ><h3 class=\"widget-title\">PiggyBac<\/h3><img loading=\"lazy\" decoding=\"async\" width=\"357\" height=\"207\" alt=\"PiggyBac-on-BAC illustration\" class=\"attachment-full\" style=\"max-width: 100%;\" srcset=\"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-content\/uploads\/sites\/134\/2024\/02\/Picture1.png 357w, https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-content\/uploads\/sites\/134\/2024\/02\/Picture1-300x174.png 300w\" sizes=\"(max-width: 357px) 100vw, 357px\" src=\"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-content\/uploads\/sites\/134\/2024\/02\/Picture1.png\" \/><\/div><\/div><\/div><\/div>","protected":false},"excerpt":{"rendered":"<p>New Services Piggy Bac-mediated transgenesis offers many advantages over traditional transgenics. It has a large cargo capacity (up to 200 kb), allowing insertion of large promoters and regulatory elements. The insertion is footprint-free and does not leave behind vector sequences. The transgene is also inserted in the host genome in a precise manner as a [&hellip;]<\/p>\n","protected":false},"author":249,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"wds_primary_category":0,"footnotes":""},"categories":[1],"tags":[],"acf":[],"publishpress_future_action":{"enabled":false,"date":"2026-04-26 03:12:35","action":"change-status","newStatus":"draft","terms":[],"taxonomy":"category"},"_links":{"self":[{"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/posts\/991"}],"collection":[{"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/users\/249"}],"replies":[{"embeddable":true,"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/comments?post=991"}],"version-history":[{"count":18,"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/posts\/991\/revisions"}],"predecessor-version":[{"id":1237,"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/posts\/991\/revisions\/1237"}],"wp:attachment":[{"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/media?parent=991"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/categories?post=991"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/tags?post=991"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}