{"id":86,"date":"2017-04-17T15:47:58","date_gmt":"2017-04-17T19:47:58","guid":{"rendered":"https:\/\/health.uconn.edu\/mouse-genome-modification\/?page_id=86"},"modified":"2018-05-08T11:33:43","modified_gmt":"2018-05-08T15:33:43","slug":"design-and-generation-of-targeting-constructs-using-recombineering-based-method","status":"publish","type":"page","link":"https:\/\/health.uconn.edu\/mouse-genome-modification\/services\/design-and-generation-of-targeting-constructs-using-recombineering-based-method\/","title":{"rendered":"Design and Generation of Targeting Constructs Using Recombineering-based Method"},"content":{"rendered":"<div id=\"pl-86\"  class=\"panel-layout\" ><div id=\"pg-86-0\"  class=\"panel-grid panel-no-style\" ><div id=\"pgc-86-0-0\"  class=\"panel-grid-cell\" ><div id=\"panel-86-0-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"0\" ><div class=\"textwidget\"><p>We design and generate gene targeting constructs. The constructs generated in the Center for Mouse Genome Modification (CMGM)\u00a0include conventional knock-out, knock-in, conditional knock-out, knock-in subtle mutations such as a point mutation and small deletion, and other custom made constructs.<\/p>\n<p>The constructs are derived from C57Bl\/6 genomic DNA, unless investigator provides the BAC clones from other strains. We use C57Bl\/6 x 129SVEV <a href=\"https:\/\/health.uconn.edu\/mouse-genome-modification\/services\/mouse-embryonic-stem-cells\/\">F1 hybrid ES cell lines<\/a> for targeting.<\/p>\n<p>Investigators who plan to make a transgenic\/targeting construct please contact the CMGM at <a href=\"mailto:gttf@uchc.edu\">gttf@uchc.edu<\/a>.<\/p>\n<p>We will need the construct purpose- conventional or conditional knock-out, point mutation or deletion as well as the MGI number\u00a0or Genbank accession number.<\/p>\n<h2><\/h2>\n<p>The service fees cover the materials and technician time. It does not cover the construct design, trouble shooting and consultation during generation of targeting animals. Thus the project should be considered as a collaboration.<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<\/div><\/div><\/div><\/div><\/div>","protected":false},"excerpt":{"rendered":"<p>We design and generate gene targeting constructs. The constructs generated in the Center for Mouse Genome Modification (CMGM)\u00a0include conventional knock-out, knock-in, conditional knock-out, knock-in subtle mutations such as a point mutation and small deletion, and other custom made constructs. The constructs are derived from C57Bl\/6 genomic DNA, unless investigator provides the BAC clones from other [&hellip;]<\/p>\n","protected":false},"author":38,"featured_media":0,"parent":80,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"acf":[],"publishpress_future_action":{"enabled":false,"date":"2026-05-09 20:45:24","action":"change-status","newStatus":"draft","terms":[],"taxonomy":""},"_links":{"self":[{"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/pages\/86"}],"collection":[{"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/users\/38"}],"replies":[{"embeddable":true,"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/comments?post=86"}],"version-history":[{"count":5,"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/pages\/86\/revisions"}],"predecessor-version":[{"id":527,"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/pages\/86\/revisions\/527"}],"up":[{"embeddable":true,"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/pages\/80"}],"wp:attachment":[{"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/media?parent=86"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}