{"id":82,"date":"2017-04-17T15:43:35","date_gmt":"2017-04-17T19:43:35","guid":{"rendered":"https:\/\/health.uconn.edu\/mouse-genome-modification\/?page_id=82"},"modified":"2018-05-10T10:41:40","modified_gmt":"2018-05-10T14:41:40","slug":"consultation","status":"publish","type":"page","link":"https:\/\/health.uconn.edu\/mouse-genome-modification\/services\/consultation\/","title":{"rendered":"Consultation"},"content":{"rendered":"<div id=\"pl-82\"  class=\"panel-layout\" ><div id=\"pg-82-0\"  class=\"panel-grid panel-no-style\" ><div id=\"pgc-82-0-0\"  class=\"panel-grid-cell\" ><div id=\"panel-82-0-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"0\" ><div class=\"textwidget\"><h1 style=\"margin: 15.0pt 0in 7.5pt 0in\"><span style=\"font-family: 'inherit',serif;font-weight: normal\">Consultation<\/span><\/h1>\n<p style=\"margin: 0in 0in 2.0pt 0in\"><span style=\"font-family: 'Arial',sans-serif;color: black\">The Center for Mouse Genome Modification\u00a0provides a comprehensive service to generate, maintain and archive genetically modified mouse strains. Investigators and trainees are welcome to discuss their needs and proposed projects including generation of novel mouse strains by CRISPR\/Cas9-mediated editing of mouse embryos, gene targeting in ES cells, or transgenic mice by pronuclear microinjection. We provide consultation to accommodate the specific needs and nature of each project. Our services include preparation of CRISPR specific reagents, targeting vectors, transgene constructs, and manipulation of early mouse embryos or ES cells to generate novel mouse strains. We will develop simple PCR genotyping assays as well as the subsequent breeding, management and cryopreservation of various mouse lines. <\/span><\/p>\n<p style=\"margin: 0in 0in 2.0pt 0in\"><span style=\"font-family: 'Arial',sans-serif;color: black\">\u00a0<\/span><\/p>\n<p style=\"margin: 0in 0in 2.0pt 0in\"><span style=\"font-family: 'Arial',sans-serif;color: black\">Whether we will use CRISPR or ES cell targeting to generate the mouse strain is largely depending on the genomic structure of the gene of interest and the nature of proposed project. Consultation is aimed towards discussion of the specific genome modification and nature of the project. The consultation will help develop strategy, design of donor\/targeting vector and other specific molecular reagents, and to formulate a final plan to generate and manage the novel<\/span><span style=\"font-size: 10.5pt;font-family: 'Helvetica Neue';color: black\"> mouse strain.<\/span><\/p>\n<\/div><\/div><\/div><\/div><\/div>","protected":false},"excerpt":{"rendered":"<p>Consultation The Center for Mouse Genome Modification\u00a0provides a comprehensive service to generate, maintain and archive genetically modified mouse strains. Investigators and trainees are welcome to discuss their needs and proposed projects including generation of novel mouse strains by CRISPR\/Cas9-mediated editing of mouse embryos, gene targeting in ES cells, or transgenic mice by pronuclear microinjection. We [&hellip;]<\/p>\n","protected":false},"author":38,"featured_media":0,"parent":80,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"acf":[],"publishpress_future_action":{"enabled":false,"date":"2026-04-15 11:35:34","action":"change-status","newStatus":"draft","terms":[],"taxonomy":""},"_links":{"self":[{"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/pages\/82"}],"collection":[{"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/users\/38"}],"replies":[{"embeddable":true,"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/comments?post=82"}],"version-history":[{"count":9,"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/pages\/82\/revisions"}],"predecessor-version":[{"id":544,"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/pages\/82\/revisions\/544"}],"up":[{"embeddable":true,"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/pages\/80"}],"wp:attachment":[{"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/media?parent=82"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}