{"id":33,"date":"2017-04-17T14:18:24","date_gmt":"2017-04-17T18:18:24","guid":{"rendered":"https:\/\/health.uconn.edu\/mouse-genome-modification\/?page_id=33"},"modified":"2017-04-21T15:44:26","modified_gmt":"2017-04-21T19:44:26","slug":"standard-operating-procedure-for-mouse-colony-husbandry","status":"publish","type":"page","link":"https:\/\/health.uconn.edu\/mouse-genome-modification\/mouse-colony-management\/standard-operating-procedure-for-mouse-colony-husbandry\/","title":{"rendered":"Standard Operating Procedure for Mouse Colony Husbandry"},"content":{"rendered":"

Clidox Preparation<\/h2>\n
    \n
  • Mixed as a 1:5:1 dilution (1 part base, 5 parts water, 1 part activator).<\/li>\n
  • Prepared once per week.<\/li>\n
  • Use in squeeze bottle or spray bottle.<\/li>\n
  • Properly label bottle.<\/li>\n
  • To be used as hand and surface disinfectant.<\/li>\n
  • Sign-up sheet; initial; 1 bottle per room.<\/li>\n<\/ul>\n

    Receiving New Animals<\/h2>\n
      \n
    • Spray boxes with Clidox before entry into GTTF.<\/li>\n
    • Boxes are sprayed a second time in Laminar Flow Hood.<\/li>\n
    • Examine overall exterior condition of box.<\/li>\n
    • Check receiver information with label on box.<\/li>\n
    • Check contents on box ( ex: correct strain, sex and overall health).<\/li>\n
    • Notify CCM office of any problems.<\/li>\n
    • Follow special husbandry procedures on receiver.<\/li>\n
    • House mice appropriately (CCM standard operating procedures).<\/li>\n
    • Complete receiver (number of mice received and number of cages).<\/li>\n
    • Add total number of cages to inventory sheet.<\/li>\n
    • Bring receiver to CCM office.<\/li>\n<\/ul>\n

      Use of Shoe Covers<\/h2>\n
        \n
      • Shoe covers need to be changed when exiting or entering the GTTF hallway.<\/li>\n<\/ul>\n

        Changing Cages (Micro-Isolators)<\/h2>\n
          \n
        • Alternate major change\/partial major change every other week.<\/li>\n
        • A minor change being the second change for the week.<\/li>\n
        • Add a nestlet if cage does not contain nesting material.<\/li>\n
        • Shelf racks replaced every four weeks.<\/li>\n<\/ul>\n

          Sterile Procedures for Entering Cages<\/h2>\n
            \n
          • New Clidox prepared every week (1:5:1 dilution).<\/li>\n
          • Soak cotton cloth with Clidox; wipe interior of Laminar Flow Hood.<\/li>\n
          • Discard cloth; wait five minutes.<\/li>\n
          • Wet a new cotton cloth with Clidox and leave in hood for disinfecting hands in between cages (continually soaking cloth).<\/li>\n
          • Place sterile cage under hood.<\/li>\n
          • Remove cage top; place to one side.<\/li>\n
          • Wipe hands on Clidox cloth.<\/li>\n
          • Enter into sterile micro-environment.<\/li>\n
          • Check water bottle level; level should not be full; level should be at bottom of neck of bottle.<\/li>\n
          • Remember that inside cage components are sterile; outside cage components are considered dirty; NEVER LET OUSIDE COMPONENTS TOUCH INSIDE STERILE ENVIRONMENT.<\/li>\n<\/ul>\n

            Daily Cage Monitoring<\/h2>\n
              \n
            • Observe animal health.<\/li>\n
            • Check feed and water levels.<\/li>\n
            • Change cages that appear wet or flooded.<\/li>\n
            • Report any births, sick or dead animals to colony management staff.<\/li>\n<\/ul>\n

              Special Feeding Requirements<\/h2>\n

              Necessary for weanlings and young C57Bl\/6, Transgenics, Malocclusions and overall \"sick\" mice:<\/p>\n

                \n
              • Place a sterile Petri dish with autoclaved granular Harlan 7012 diet on floor of cage.<\/li>\n
              • Replace as needed.<\/li>\n
              • Put a yellow \"monitor food\" card on cage.<\/li>\n
              • Gel food may be added to cage in a sterile Petri dish for mice that appear dehydrated.<\/li>\n<\/ul>\n

                Pregnant Mice<\/h2>\n
                  \n
                • Place a \"please separate\" card on cage for harem mating units.<\/li>\n
                • Colony management staff will separate females and provide a nestlet.<\/li>\n
                • Do not change cage two days before birth.<\/li>\n
                • Do not change cage until pups are at least three days old.<\/li>\n
                • Pups are transferred into clean cage with previous nestlet if not excessively soiled.<\/li>\n
                • Place food on cage bottom.<\/li>\n<\/ul>\n

                  Recording Litters<\/h2>\n
                    \n
                  • Note date of litter on birth card.<\/li>\n
                  • Write \"wean\" date; 21 days from birth date.<\/li>\n
                  • Inform Colony Management staff of new litters or missing litters.<\/li>\n
                  • Turn card around to \"please wean\" at 21 days.<\/li>\n
                  • Don't count litter number until day three or more.<\/li>\n
                  • If litter needs to be fostered notify Colony Management staff of birth.<\/li>\n<\/ul>\n

                    Supplies<\/h2>\n
                      \n
                    • Sterile supplies and caging may be brought into the GTTF and rolled across sticky mats.<\/li>\n
                    • Upon returning from the pass-through room shoe covers are taken off before walking through GTTF corridor and discarded.<\/li>\n<\/ul>\n

                      Cleanliness of Work Area<\/h2>\n
                        \n
                      • Clean Laminar Flow Hood after use with Clidox then 70 percent alcohol.<\/li>\n
                      • Sweep floor after changing cages.<\/li>\n
                      • Mop floor with Quatricide weekly.<\/li>\n
                      • Clean sinks and counters weekly.<\/li>\n
                      • Maintain GTTF hallway weekly.<\/li>\n
                      • Check operational function of air purifiers in Procedure Rooms.<\/li>\n
                      • Change wall filters every three months.<\/li>\n
                      • Sanitize rooms every six months.<\/li>\n
                      • Pull sticky mats daily.<\/li>\n
                      • Racks placed in hallway for removal of dirty cages.<\/li>\n<\/ul>\n

                        Exiting Dirty Supplies To Washroom<\/h2>\n
                          \n
                        • Cover all caging.<\/li>\n
                        • Remove plastic bags from water bottles.<\/li>\n
                        • Leave dirty caging at end of GTTF hallway; do not exit caging until you are finished with daily husbandry work.<\/li>\n
                        • Once cages are sent outside of GTTF double-doors; remove shoe covers before walking down clean corridor.<\/li>\n<\/ul>\n

                          Supplies Entering Facility<\/h2>\n
                            \n
                          • All items enter at East Dock end of hallway.<\/li>\n
                          • Spray with Clidox 1:5:1 dilution.<\/li>\n
                          • Whenever possible wait 48 hours before bringing into facility.<\/li>\n<\/ul>\n

                            Contributed by Kelly Hoyt.<\/em><\/p>\n<\/div><\/div><\/div><\/div><\/div>","protected":false},"excerpt":{"rendered":"

                            Clidox PreparationMixed as a 1:5:1 dilution (1 part base, 5 parts water, 1 part activator).Prepared once per week.Use in squeeze bottle or spray bottle.Properly label bottle.To be used as hand and surface disinfectant.Sign-up sheet; initial; 1 bottle per room.Receiving New AnimalsSpray boxes with Clidox before entry into GTTF.Boxes are sprayed a second time in Laminar […]<\/p>\n","protected":false},"author":38,"featured_media":0,"parent":29,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"acf":[],"publishpress_future_action":{"enabled":false,"date":"2026-04-26 05:38:10","action":"change-status","newStatus":"draft","terms":[],"taxonomy":""},"_links":{"self":[{"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/pages\/33"}],"collection":[{"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/users\/38"}],"replies":[{"embeddable":true,"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/comments?post=33"}],"version-history":[{"count":3,"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/pages\/33\/revisions"}],"predecessor-version":[{"id":306,"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/pages\/33\/revisions\/306"}],"up":[{"embeddable":true,"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/pages\/29"}],"wp:attachment":[{"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/media?parent=33"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}