{"id":2,"date":"2017-03-31T14:03:08","date_gmt":"2017-03-31T18:03:08","guid":{"rendered":"https:\/\/health.uconn.edu\/mouse-genome-modification\/?page_id=2"},"modified":"2025-10-14T13:23:18","modified_gmt":"2025-10-14T17:23:18","slug":"home","status":"publish","type":"page","link":"https:\/\/health.uconn.edu\/mouse-genome-modification\/","title":{"rendered":"Home"},"content":{"rendered":"<div id=\"pl-2\"  class=\"panel-layout\" ><div id=\"pg-2-0\"  class=\"panel-grid panel-no-style\" ><div id=\"pgc-2-0-0\"  class=\"panel-grid-cell\" ><div id=\"panel-2-0-0-0\" class=\"so-panel widget widget_metaslider_widget panel-first-child panel-last-child\" data-index=\"0\" ><div id=\"metaslider-id-330\" style=\"width: 100%; margin: 0 auto;\" class=\"ml-slider-3-80-0 ml-slider-pro-2-36-0 metaslider metaslider-flex metaslider-330 ml-slider ms-theme-default nav-hidden\" role=\"region\" aria-roledescription=\"Slideshow\" aria-label=\"Home\">\n    <div id=\"metaslider_container_330\">\n        <div id=\"metaslider_330\">\n            <ul class='slides'>\n                <li style=\"display: block; width: 100%;\" class=\"slide-329 ms-image \" aria-roledescription=\"slide\" aria-label=\"slide-329\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-content\/uploads\/sites\/134\/2017\/04\/shutterstock_9557542.jpg\" height=\"350\" width=\"1170\" alt=\"DNA strand\" class=\"slider-330 slide-329\" \/><\/li>\n                <li style=\"display: none; width: 100%;\" class=\"slide-327 ms-image \" aria-roledescription=\"slide\" aria-label=\"slide-327\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-content\/uploads\/sites\/134\/2017\/04\/DSC_0001.jpg\" height=\"350\" width=\"1170\" alt=\"Researcher looking into a petri dish through a microscope\" class=\"slider-330 slide-327\" \/><\/li>\n                <li style=\"display: none; width: 100%;\" class=\"slide-353 ms-image \" aria-roledescription=\"slide\" aria-label=\"slide-353\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-content\/uploads\/sites\/134\/2017\/05\/IMG_5416-1170x350.jpg\" height=\"350\" width=\"1170\" alt=\"Eggs\" class=\"slider-330 slide-353\" \/><\/li>\n                <li style=\"display: none; width: 100%;\" class=\"slide-331 ms-image \" aria-roledescription=\"slide\" aria-label=\"slide-331\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-content\/uploads\/sites\/134\/2017\/04\/IMG_5248.jpg\" height=\"350\" width=\"1170\" alt=\"Egg\" class=\"slider-330 slide-331\" \/><\/li>\n                <li style=\"display: none; width: 100%;\" class=\"slide-332 ms-image \" aria-roledescription=\"slide\" aria-label=\"slide-332\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-content\/uploads\/sites\/134\/2017\/04\/IMG_5249.jpg\" height=\"350\" width=\"1170\" alt=\"Egg\" class=\"slider-330 slide-332\" \/><\/li>\n                <li style=\"display: none; width: 100%;\" class=\"slide-326 ms-image \" aria-roledescription=\"slide\" aria-label=\"slide-326\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-content\/uploads\/sites\/134\/2017\/04\/artificial_insemination_shutterstock_129449231.jpg\" height=\"350\" width=\"1170\" alt=\"Artificial insemination\" class=\"slider-330 slide-326\" \/><\/li>\n                <li style=\"display: none; width: 100%;\" class=\"slide-325 ms-image \" aria-roledescription=\"slide\" aria-label=\"slide-325\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-content\/uploads\/sites\/134\/2017\/04\/Academic_Ent_6-16-16_JGelineau146.jpg\" height=\"350\" width=\"1170\" alt=\"UConn Health sign on the building\" class=\"slider-330 slide-325\" \/><\/li>\n            <\/ul>\n        <\/div>\n        \n    <\/div>\n<\/div><\/div><\/div><\/div><div id=\"pg-2-1\"  class=\"panel-grid panel-no-style\" ><div id=\"pgc-2-1-0\"  class=\"panel-grid-cell\" ><div id=\"panel-2-1-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"1\" ><div class=\"intro-text panel-widget-style panel-widget-style-for-2-1-0-0\" ><div class=\"textwidget\"><p>The Center for Mouse Genome Modification (CMGM) at UConn Health provides design and generation of genetically modified mice and other services.\u00a0 See about the services we have to offer on our QuickTake video:<\/p>\n<p>https:\/\/uconnhealth.mediasite.com\/Mediasite\/Play\/a0b3096027264469b6fc039ebc0d642c1d<\/p>\n<\/div><\/div><\/div><\/div><\/div><div id=\"pg-2-2\"  class=\"panel-grid panel-has-style\" ><div class=\"panel-row-style panel-row-style-for-2-2\" ><div id=\"pgc-2-2-0\"  class=\"panel-grid-cell panel-grid-cell-empty\" ><\/div><\/div><\/div><div id=\"pg-2-3\"  class=\"panel-grid panel-no-style\" ><div id=\"pgc-2-3-0\"  class=\"panel-grid-cell\" ><div id=\"panel-2-3-0-0\" class=\"so-panel widget widget_siteorigin-panels-postloop panel-first-child panel-last-child\" data-index=\"2\" ><div class=\"post-hr panel-widget-style panel-widget-style-for-2-3-0-0\" ><article id=\"post-1153\" class=\"post-1153 post type-post status-publish format-standard hentry category-uncategorized\">\r\n\t<header class=\"entry-header\">\r\n\t\t\t\t\r\n\t\t<h1 class=\"entry-title\">Recombinant Adeno-Associated Viruses (rAAV)<\/h1>\t\t<div class=\"entry-meta\">\r\n\t\t\t<span class=\"posted-on\">Posted on <a href=\"https:\/\/health.uconn.edu\/mouse-genome-modification\/2024\/03\/12\/recombinant-adeno-associated-viruses-raav\/\" rel=\"bookmark\"><time class=\"entry-date published\" datetime=\"2024-03-12T14:22:27-04:00\">March 12, 2024<\/time><time class=\"updated\" datetime=\"2025-07-01T08:55:15-04:00\">July 1, 2025<\/time><\/a><\/span><span class=\"byline\"> by <span class=\"author vcard\"><a class=\"url fn n\" href=\"https:\/\/health.uconn.edu\/mouse-genome-modification\/author\/dmc04013\/\">Deborah M Kaback<\/a><\/span><\/span>\t\t<\/div><!-- .entry-meta -->\r\n\t\t\r\n\t\t\t\t\r\n\t<\/header><!-- .entry-header -->\r\n\t\r\n\t<div class=\"entry-content clearfix subpage\">\r\n\r\n\r\n        <p><a href=\"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-content\/uploads\/sites\/134\/2024\/03\/rAAV.png\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-content\/uploads\/sites\/134\/2024\/03\/rAAV-300x129.png\" alt=\"\" width=\"363\" height=\"156\" class=\"alignnone wp-image-1164\" srcset=\"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-content\/uploads\/sites\/134\/2024\/03\/rAAV-300x129.png 300w, https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-content\/uploads\/sites\/134\/2024\/03\/rAAV-1024x441.png 1024w, https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-content\/uploads\/sites\/134\/2024\/03\/rAAV-768x331.png 768w, https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-content\/uploads\/sites\/134\/2024\/03\/rAAV.png 1133w\" sizes=\"(max-width: 363px) 100vw, 363px\" \/><\/a><\/p>\n<p>The Center for Mouse Genome Modification (CMGM) is now offering genome editing services using recombinant adeno-associated viruses (rAAV).<\/p>\n<p>AAV (adeno-associated virus), which is a single-stranded DNA (ssDNA) virus, can effectively penetrate the zona pellucida and infect one-cell embryos. Recombinant AAV (rAAV) contains the sequence of interest together with 5\u2019- and 3\u2019-homology arms for CRISPR-mediated gene editing in the mouse genome. To this end, we infect one-cell embryos with rAAV followed by electroporation of with Cas9\/sgRNA ribonucleoprotein (RNP) for subsequent genome modification in the mouse. The advantage of this approach is that rAAV infection is faster and less laborious than traditional pronuclear microinjection to transduce ssDNA template into mouse embryos. Furthermore, ssDNA template in the form of rAAV genome is a more efficient template for homology-directed repair as compared to double-stranded DNA. This approach significantly increases the efficiency to generate novel mouse models containing the sequence of interest.<\/p>\n\r\n        \r\n\r\n\t\t<hr\/>\r\n\t\t\t<\/div><!-- .entry-content -->\r\n\r\n\t<footer class=\"entry-footer\">\r\n\t\t\r\n\t\t\r\n\t<\/footer><!-- .entry-footer -->\r\n<\/article><!-- #post-## -->\r\n<\/div><\/div><\/div><div id=\"pgc-2-3-1\"  class=\"panel-grid-cell\" ><div id=\"panel-2-3-1-0\" class=\"so-panel widget widget_siteorigin-panels-postloop panel-first-child panel-last-child\" data-index=\"3\" ><div class=\"post-hr panel-widget-style panel-widget-style-for-2-3-1-0\" ><article id=\"post-991\" class=\"post-991 post type-post status-publish format-standard hentry category-uncategorized\">\r\n\t<header class=\"entry-header\">\r\n\t\t\t\t\r\n\t\t<h1 class=\"entry-title\">Piggy Bac Mediated Transgenesis<\/h1>\t\t<div class=\"entry-meta\">\r\n\t\t\t<span class=\"posted-on\">Posted on <a href=\"https:\/\/health.uconn.edu\/mouse-genome-modification\/2022\/02\/02\/piggybac-on-bac\/\" rel=\"bookmark\"><time class=\"entry-date published\" datetime=\"2022-02-02T10:43:16-05:00\">February 2, 2022<\/time><time class=\"updated\" datetime=\"2025-07-01T08:54:43-04:00\">July 1, 2025<\/time><\/a><\/span><span class=\"byline\"> by <span class=\"author vcard\"><a class=\"url fn n\" href=\"https:\/\/health.uconn.edu\/mouse-genome-modification\/author\/dmc04013\/\">Deborah M Kaback<\/a><\/span><\/span>\t\t<\/div><!-- .entry-meta -->\r\n\t\t\r\n\t\t\t\t\r\n\t<\/header><!-- .entry-header -->\r\n\t\r\n\t<div class=\"entry-content clearfix subpage\">\r\n\r\n\r\n        <div id=\"pl-991\"  class=\"panel-layout\" ><div id=\"pg-991-0\"  class=\"panel-grid panel-no-style\" ><div id=\"pgc-991-0-0\"  class=\"panel-grid-cell\" ><div id=\"panel-991-0-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child\" data-index=\"0\" ><div class=\"textwidget\"><h2>New Services<\/h2>\n<p>Piggy Bac-mediated transgenesis offers many advantages over traditional transgenics. It has a large cargo capacity (up to 200 kb), allowing insertion of large promoters and regulatory elements. The insertion is footprint-free and does not leave behind vector sequences. The transgene is also inserted in the host genome in a precise manner as a single copy without any chromosomal rearrangement or deletion (chromothripsis) and therefore offers reliable and consistent transgene expression.<\/p>\n<p>In the Piggy Bac approach, the donor template includes the transgene of interest flanked by inverted terminal repeat (ITR) sequences. The donor template is microinjected into 1-cell embryos along with mRNA encoding the transposase, which integrates the transgene into the host genome through a \u201ccut and paste\u201d mechanism.<\/p>\n<p>The CMGM has generated several novel mouse models using this approach including cell specific ablation and conditional rescue humanized mouse models.<\/p>\n<\/div><\/div><div id=\"panel-991-0-0-1\" class=\"so-panel widget widget_widget_sp_image widget_sp_image panel-last-child\" data-index=\"1\" ><h3 class=\"widget-title\">PiggyBac<\/h3><img loading=\"lazy\" decoding=\"async\" width=\"357\" height=\"207\" alt=\"PiggyBac-on-BAC illustration\" class=\"attachment-full\" style=\"max-width: 100%;\" srcset=\"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-content\/uploads\/sites\/134\/2024\/02\/Picture1.png 357w, https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-content\/uploads\/sites\/134\/2024\/02\/Picture1-300x174.png 300w\" sizes=\"(max-width: 357px) 100vw, 357px\" src=\"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-content\/uploads\/sites\/134\/2024\/02\/Picture1.png\" \/><\/div><\/div><\/div><\/div>\r\n        \r\n\r\n\t\t<hr\/>\r\n\t\t\t<\/div><!-- .entry-content -->\r\n\r\n\t<footer class=\"entry-footer\">\r\n\t\t\r\n\t\t\r\n\t<\/footer><!-- .entry-footer -->\r\n<\/article><!-- #post-## -->\r\n<\/div><\/div><\/div><\/div><\/div>","protected":false},"excerpt":{"rendered":"<p>New Services Piggy Bac-mediated transgenesis offers many advantages over traditional transgenics. It has a large cargo capacity (up to 200 kb), allowing insertion of large promoters and regulatory elements. The insertion is footprint-free and does not leave behind vector sequences. The transgene is also inserted in the host genome in a precise manner as a [&hellip;]<\/p>\n","protected":false},"author":38,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"open","template":"page-blank.php","meta":{"_acf_changed":false,"footnotes":""},"acf":[],"publishpress_future_action":{"enabled":false,"date":"2026-05-10 18:42:12","action":"change-status","newStatus":"draft","terms":[],"taxonomy":""},"_links":{"self":[{"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/pages\/2"}],"collection":[{"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/users\/38"}],"replies":[{"embeddable":true,"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/comments?post=2"}],"version-history":[{"count":35,"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/pages\/2\/revisions"}],"predecessor-version":[{"id":1258,"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/pages\/2\/revisions\/1258"}],"wp:attachment":[{"href":"https:\/\/health.uconn.edu\/mouse-genome-modification\/wp-json\/wp\/v2\/media?parent=2"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}