{"id":16,"date":"2017-06-01T13:19:47","date_gmt":"2017-06-01T17:19:47","guid":{"rendered":"https:\/\/health.uconn.edu\/li-lab\/?page_id=16"},"modified":"2017-06-20T15:36:56","modified_gmt":"2017-06-20T19:36:56","slug":"protocols","status":"publish","type":"page","link":"https:\/\/health.uconn.edu\/li-lab\/protocols\/","title":{"rendered":"Protocols"},"content":{"rendered":"

Neurobiology<\/h3>\n

Axon Tracing with DiI\/DiA<\/a><\/p>\n

Brain Slice Culture<\/a><\/p>\n

Explant Culture in Matrigel<\/a><\/p>\n

Neuospere Culture<\/a><\/p>\n

Electroporation in Brain Slice<\/p>\n

In Utero Electroporation<\/a><\/p>\n<\/div><\/div>

Staining<\/h3>\n

TUNEL Assay<\/a><\/p>\n

Section ISH<\/a><\/p>\n

Immunofluorescence<\/a><\/p>\n

X-gal Staining<\/a><\/p>\n

BrdU Immunohistochemistry<\/a><\/p>\n

Wholemount ISH<\/a><\/p>\n

Immunocytochemistry<\/a><\/p>\n

H and E Staining<\/a><\/p>\n

BrdU Immunofluorescence<\/a><\/p>\n

Neurofilament Staining<\/a><\/p>\n

Synthesis of RNA Probes<\/a><\/p>\n

Nissl Staining<\/p>\n<\/div><\/div>

Molecular and Cell Biology<\/h3>\n

ChIP Assay<\/a><\/p>\n

Protein Quantification<\/a><\/p>\n

Mini-prep<\/a><\/p>\n

Total RNA extraction<\/a><\/p>\n

Gel Shift Assay<\/p>\n

Western Blot<\/p>\n

Heat Shock Transformation<\/a><\/p>\n

\"One Minute\" Transformation<\/a><\/p>\n

Extraction of Nuclear Proteins<\/a><\/p>\n

Transfection with Lipofectamine 2000<\/a><\/p>\n

DNA Probes for Southern Blot<\/a><\/p>\n

Electroporation<\/a><\/p>\n<\/div><\/div><\/div>

BAC Modification<\/h3>\n

Preparation of Electrocompetent Cells<\/a><\/p>\n

Large Prep of BAC DNA (CsCl)<\/a><\/p>\n

Prep BAC DNA for Pronuclear Injection<\/a><\/p>\n

BAC DNA Isolation<\/a><\/p>\n

Preparation of Recombination Competent EL350\/EL250 Cells<\/a><\/p>\n

Mini Prep of BAC DNA <\/a><\/p>\n

Retrieving DNA by Gap Repairing<\/a><\/p>\n<\/div><\/div>

Mouse Embryonic Stem (ES) Cell Manipulations<\/h3>\n

ES Cell Culture<\/p>\n

STO Cell Feeder Plate<\/a><\/p>\n

Mini-Southern Blot Analysis<\/a><\/p>\n

Gene Targeting in ES Cells<\/a><\/p>\n

Preparation of Feeder Cells<\/a><\/p>\n<\/div><\/div>

Manipulations of Mouse Embryos<\/h3>\n

Morula Aggregation<\/a><\/p>\n

Preparation of DNA Fragment for Production of Transgenic Mouse<\/a><\/p>\n

Superovulation<\/a><\/p>\n

PCR Genotyping<\/a><\/p>\n

Fertilized Eggs Collection<\/a><\/p>\n<\/div><\/div>

Manipulation of Chick Embryos<\/h3>\n

Electroporation in Chick Embryos<\/a><\/p>\n<\/div><\/div>

Molecular Biology<\/h3>\n

Colony Screening by PCR<\/a><\/p>\n

Total RNA Extraction<\/a><\/p>\n

Preparation of Chemical Competent Cells<\/a><\/p>\n

Preparation of Electro-competent Cells<\/a><\/p>\n<\/div><\/div><\/div><\/div><\/div>","protected":false},"excerpt":{"rendered":"

NeurobiologyAxon Tracing with DiI\/DiABrain Slice CultureExplant Culture in MatrigelNeuospere CultureElectroporation in Brain SliceIn Utero Electroporation StainingTUNEL AssaySection ISHImmunofluorescenceX-gal StainingBrdU ImmunohistochemistryWholemount ISHImmunocytochemistryH and E StainingBrdU ImmunofluorescenceNeurofilament StainingSynthesis of RNA ProbesNissl Staining Molecular and Cell BiologyChIP AssayProtein QuantificationMini-prepTotal RNA extractionGel Shift AssayWestern BlotHeat Shock Transformation”One Minute” TransformationExtraction of Nuclear ProteinsTransfection with Lipofectamine 2000DNA Probes for Southern BlotElectroporation<\/p>\n","protected":false},"author":39,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"acf":[],"publishpress_future_action":{"enabled":false,"date":"2026-04-17 12:05:26","action":"change-status","newStatus":"draft","terms":[],"taxonomy":""},"_links":{"self":[{"href":"https:\/\/health.uconn.edu\/li-lab\/wp-json\/wp\/v2\/pages\/16"}],"collection":[{"href":"https:\/\/health.uconn.edu\/li-lab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/health.uconn.edu\/li-lab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/health.uconn.edu\/li-lab\/wp-json\/wp\/v2\/users\/39"}],"replies":[{"embeddable":true,"href":"https:\/\/health.uconn.edu\/li-lab\/wp-json\/wp\/v2\/comments?post=16"}],"version-history":[{"count":7,"href":"https:\/\/health.uconn.edu\/li-lab\/wp-json\/wp\/v2\/pages\/16\/revisions"}],"predecessor-version":[{"id":115,"href":"https:\/\/health.uconn.edu\/li-lab\/wp-json\/wp\/v2\/pages\/16\/revisions\/115"}],"wp:attachment":[{"href":"https:\/\/health.uconn.edu\/li-lab\/wp-json\/wp\/v2\/media?parent=16"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}