{"id":21,"date":"2015-11-23T13:26:54","date_gmt":"2015-11-23T18:26:54","guid":{"rendered":"http:\/\/production.wordpress.uconn.edu\/uctemplate2014\/?page_id=21"},"modified":"2025-12-03T09:02:13","modified_gmt":"2025-12-03T14:02:13","slug":"home","status":"publish","type":"page","link":"https:\/\/health.uconn.edu\/human-genome\/","title":{"rendered":"Home"},"content":{"rendered":"<div id=\"pl-21\"  class=\"panel-layout\" ><div id=\"pg-21-0\"  class=\"panel-grid panel-no-style\" ><div id=\"pgc-21-0-0\"  class=\"panel-grid-cell\" ><div id=\"panel-21-0-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"0\" ><div class=\"textwidget\"><h2><strong>Genome Editing<\/strong><\/h2>\n<p style=\"font-weight: 400;\">The Cell and Genome Engineering Core (CGEC) supports the university research community by providing state-of-the-art genome editing services and scientific expertise. Our mission is to empower investigators with high-quality, customized cellular models that accelerate discovery and innovation.<\/p>\n<p style=\"font-weight: 400;\">We offer full-service TALEN and CRISPR\/Cas9 strategies to engineer cell lines for a wide variety of research applications. Our capabilities include:<\/p>\n<ul>\n<li style=\"font-weight: 400;\">Precise single\u2013base pair edits to introduce or correct patient-specific mutations<\/li>\n<li style=\"font-weight: 400;\">Targeted deletions, ranging from a few bases to multi-megabase regions, for knockout model generation<\/li>\n<li style=\"font-weight: 400;\">Reporter and fusion-protein knock-ins at any genomic locus<\/li>\n<li style=\"font-weight: 400;\">Safe-harbor locus engineering to support reliable expression of exogenous DNA<\/li>\n<li style=\"font-weight: 400;\">Lentiviral delivery options for genome editing in hard-to-transfect or suspension cancer cell lines<\/li>\n<\/ul>\n<h3><strong>Knockouts and Targeted Deletions<\/strong><\/h3>\n<ul>\n<li style=\"font-weight: 400;\">Targeted sequence deletions ranging from a few bases to multi-megabase regions<\/li>\n<li style=\"font-weight: 400;\">Knockout generation in hard-to-transfect cancer cell lines using lentiviral delivery systems<\/li>\n<\/ul>\n<h3><strong>Knock-ins and Reporter Engineering<\/strong><\/h3>\n<ul>\n<li style=\"font-weight: 400;\">Targeted knock-in of reporters, fusion tags, and functional elements<\/li>\n<li style=\"font-weight: 400;\">Design and integration of custom reporter constructs<\/li>\n<li style=\"font-weight: 400;\">Safe-harbor locus engineering for stable and consistent expression of exogenous DNA<\/li>\n<\/ul>\n<p style=\"font-weight: 400;\">We specialize in scarless genome editing using optimized single-stranded or HDR-based templates. For custom reporter constructs, we can design, build, and target vectors tailored to your laboratory\u2019s research needs.<\/p>\n<p style=\"font-weight: 400;\">Contact us at <u>ucscicore@uchc.edu<\/u> to discuss your project and explore how we can support your scientific goals.<\/p>\n<p>Additional information:<\/p>\n<p><a href=\"https:\/\/health.uconn.edu\/human-genome\/crisprcas9\/\">CRISPR\/Cas9<\/a><\/p>\n<p><a href=\"https:\/\/health.uconn.edu\/human-genome\/targeting-vectors\/\">Targeting Vectors<\/a><\/p>\n<p><a href=\"https:\/\/health.uconn.edu\/human-genome\/cell-culture-services\/\" class=\"broken_link\">Cell Culture\u00a0Services<\/a><\/p>\n<\/div><\/div><\/div><div id=\"pgc-21-0-1\"  class=\"panel-grid-cell\" ><div id=\"panel-21-0-1-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"1\" ><div class=\"textwidget\"><p>&nbsp;<\/p>\n<h3><strong>Genome Editing Timeline<\/strong><\/h3>\n<p>&nbsp;<\/p>\n<p><a href=\"https:\/\/health.uconn.edu\/human-genome\/wp-content\/uploads\/sites\/126\/2025\/12\/Screenshot-2025-12-03-at-8.56.57\u202fAM.png\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/health.uconn.edu\/human-genome\/wp-content\/uploads\/sites\/126\/2025\/12\/Screenshot-2025-12-03-at-8.56.57\u202fAM-300x125.png\" alt=\"\" width=\"485\" height=\"202\" class=\"alignnone wp-image-128\" srcset=\"https:\/\/health.uconn.edu\/human-genome\/wp-content\/uploads\/sites\/126\/2025\/12\/Screenshot-2025-12-03-at-8.56.57\u202fAM-300x125.png 300w, https:\/\/health.uconn.edu\/human-genome\/wp-content\/uploads\/sites\/126\/2025\/12\/Screenshot-2025-12-03-at-8.56.57\u202fAM-1024x427.png 1024w, https:\/\/health.uconn.edu\/human-genome\/wp-content\/uploads\/sites\/126\/2025\/12\/Screenshot-2025-12-03-at-8.56.57\u202fAM-768x321.png 768w, https:\/\/health.uconn.edu\/human-genome\/wp-content\/uploads\/sites\/126\/2025\/12\/Screenshot-2025-12-03-at-8.56.57\u202fAM.png 1078w\" sizes=\"(max-width: 485px) 100vw, 485px\" \/><\/a><\/p>\n<\/div><\/div><\/div><\/div><\/div>","protected":false},"excerpt":{"rendered":"<p>Genome Editing The Cell and Genome Engineering Core (CGEC) supports the university research community by providing state-of-the-art genome editing services and scientific expertise. Our mission is to empower investigators with high-quality, customized cellular models that accelerate discovery and innovation. We offer full-service TALEN and CRISPR\/Cas9 strategies to engineer cell lines for a wide variety of [&hellip;]<\/p>\n","protected":false},"author":38,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"open","template":"page-blank.php","meta":{"_acf_changed":false,"footnotes":""},"acf":[],"publishpress_future_action":{"enabled":false,"date":"2026-05-05 11:50:55","action":"change-status","newStatus":"draft","terms":[],"taxonomy":""},"_links":{"self":[{"href":"https:\/\/health.uconn.edu\/human-genome\/wp-json\/wp\/v2\/pages\/21"}],"collection":[{"href":"https:\/\/health.uconn.edu\/human-genome\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/health.uconn.edu\/human-genome\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/health.uconn.edu\/human-genome\/wp-json\/wp\/v2\/users\/38"}],"replies":[{"embeddable":true,"href":"https:\/\/health.uconn.edu\/human-genome\/wp-json\/wp\/v2\/comments?post=21"}],"version-history":[{"count":15,"href":"https:\/\/health.uconn.edu\/human-genome\/wp-json\/wp\/v2\/pages\/21\/revisions"}],"predecessor-version":[{"id":138,"href":"https:\/\/health.uconn.edu\/human-genome\/wp-json\/wp\/v2\/pages\/21\/revisions\/138"}],"wp:attachment":[{"href":"https:\/\/health.uconn.edu\/human-genome\/wp-json\/wp\/v2\/media?parent=21"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}