{"id":22,"date":"2017-04-27T10:10:49","date_gmt":"2017-04-27T14:10:49","guid":{"rendered":"https:\/\/health.uconn.edu\/flow-cytometry\/?page_id=22"},"modified":"2024-08-20T13:47:45","modified_gmt":"2024-08-20T17:47:45","slug":"instrumentation","status":"publish","type":"page","link":"https:\/\/health.uconn.edu\/flow-cytometry\/instrumentation\/","title":{"rendered":"Instrumentation"},"content":{"rendered":"<div id=\"pl-22\"  class=\"panel-layout\" ><div id=\"pg-22-0\"  class=\"panel-grid panel-no-style\" ><div id=\"pgc-22-0-0\"  class=\"panel-grid-cell\" ><div id=\"panel-22-0-0-0\" class=\"so-panel widget widget_widget_sp_image widget_sp_image panel-first-child panel-last-child\" data-index=\"0\" ><img loading=\"lazy\" decoding=\"async\" width=\"250\" height=\"150\" alt=\"Accuri C6 high throughput system\" class=\"attachment-full\" style=\"max-width: 100%;\" src=\"https:\/\/health.uconn.edu\/flow-cytometry\/wp-content\/uploads\/sites\/123\/2018\/06\/accuri_c6_silo.jpg\" \/><\/div><\/div><div id=\"pgc-22-0-1\"  class=\"panel-grid-cell\" ><div id=\"panel-22-0-1-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"1\" ><div class=\"textwidget\"><h3>Accuri\u2122 C6<\/h3>\n<p>Located in room E3004, this simple high throughput system is equipped with a blue and red laser, two light scatter detectors, and four fluorescence detectors with optical filters optimized for the detection of FITC, PE, PerCP, and APC. A compact optical design, fixed alignment, and preoptimized detector settings make the system easier to use. Data is digitally collected over a wide dynamic range of\u00a0 7.2 decades (16 million channels of digital data), making\u00a0 all data available to users as needed. Gating strategies and\u00a0 fluorescence compensation values can be set before, during, or after data collection.<\/p>\n<ul>\n<li><a href=\"https:\/\/health.uconn.edu\/flow-cytometry\/wp-content\/uploads\/sites\/123\/2018\/06\/QuickStart-Guide-Collect.pdf\">Quick Start Guide: Collect<\/a><\/li>\n<li><a href=\"https:\/\/health.uconn.edu\/flow-cytometry\/wp-content\/uploads\/sites\/123\/2018\/06\/QuickStart-Guide-Analyze.pdf\">Quick Start Guide: Analyze<\/a><\/li>\n<li><a href=\"https:\/\/health.uconn.edu\/flow-cytometry\/wp-content\/uploads\/sites\/123\/2018\/06\/7820019-QuickStart-Guide-CFlow-Sampler.pdf\">Quick Start Guide: CFlow\u00ae Sampler<\/a><\/li>\n<\/ul>\n<\/div><\/div><\/div><\/div><div id=\"pg-22-1\"  class=\"panel-grid panel-no-style\" ><div id=\"pgc-22-1-0\"  class=\"panel-grid-cell\" ><div id=\"panel-22-1-0-0\" class=\"so-panel widget widget_widget_sp_image widget_sp_image panel-first-child panel-last-child\" data-index=\"2\" ><img loading=\"lazy\" decoding=\"async\" width=\"250\" height=\"150\" alt=\"Amnis ImageStream X Mark II imaging flow cytometer\" class=\"attachment-full\" style=\"max-width: 100%;\" src=\"https:\/\/health.uconn.edu\/flow-cytometry\/wp-content\/uploads\/sites\/123\/2018\/06\/amnis_imagestream.jpg\" \/><\/div><\/div><div id=\"pgc-22-1-1\"  class=\"panel-grid-cell\" ><div id=\"panel-22-1-1-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"3\" ><div class=\"textwidget\"><h3>Amnis ImageStream\u00ae<sup>X<\/sup>\u00a0Mark II<\/h3>\n<p>The revolutionary ImageStream\u00ae<sup>X<\/sup> Mark II Imaging Flow Cytometer combines the speed, sensitivity, and phenotyping abilities of flow cytometry with the detailed imagery and functional insights of microscopy. This unique combination enables a broad range of applications that would be impossible using either technique alone.<\/p>\n<p>This instrument produces multiple high-resolution images of every cell directly in flow, including brightfield and darkfield (SSC), and up to 10 fluorescent markers with sensitivity exceeding conventional flow cytometers. Applications include the study of cell-cell interactions, phagocytosis, apoptosis and autophagy, the characterization of circulating tumor cells, and many others.<\/p>\n<ul>\n<li><a href=\"https:\/\/health.uconn.edu\/flow-cytometry\/wp-content\/uploads\/sites\/123\/2018\/06\/Sample-Prep-Guide_ISX.pdf\">Quick Start Guide: ISX Sample Preparation<\/a><\/li>\n<li><a href=\"https:\/\/health.uconn.edu\/flow-cytometry\/wp-content\/uploads\/sites\/123\/2018\/06\/780-00959-00-RevA_IS-MkII-QS-guide.pdf\">Quick Start Guide: ImageStream Mark II Operation<\/a><\/li>\n<\/ul>\n<\/div><\/div><\/div><\/div><div id=\"pg-22-2\"  class=\"panel-grid panel-no-style\" ><div id=\"pgc-22-2-0\"  class=\"panel-grid-cell\" ><div id=\"panel-22-2-0-0\" class=\"so-panel widget widget_widget_sp_image widget_sp_image panel-first-child panel-last-child\" data-index=\"4\" ><img loading=\"lazy\" decoding=\"async\" width=\"542\" height=\"529\" alt=\"BD FACSymphony A5 SE\" class=\"attachment-full\" style=\"max-width: 100%;\" srcset=\"https:\/\/health.uconn.edu\/flow-cytometry\/wp-content\/uploads\/sites\/123\/2024\/01\/BD-FACSymphony-A5-SE.jpg 542w, https:\/\/health.uconn.edu\/flow-cytometry\/wp-content\/uploads\/sites\/123\/2024\/01\/BD-FACSymphony-A5-SE-300x293.jpg 300w\" sizes=\"(max-width: 542px) 100vw, 542px\" src=\"https:\/\/health.uconn.edu\/flow-cytometry\/wp-content\/uploads\/sites\/123\/2024\/01\/BD-FACSymphony-A5-SE.jpg\" \/><\/div><\/div><div id=\"pgc-22-2-1\"  class=\"panel-grid-cell\" ><div id=\"panel-22-2-1-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"5\" ><div class=\"textwidget\"><h3>BD FACSymphony A5 SE<\/h3>\n<p>There are two identical BD FACSymphony A5 SE cell analyzers which have five lasers and 48 algorithmically optimized fluorescence detectors for cellular analysis. This instrument can be used for both conventional and spectral flow cytometry because it uses additional high-sensitivity detectors and new software functionality to detect the full spectrum of emitted light and unmix spectrally live in BD FACS Diva software, all while retaining the flexibility of familiar compensation-based workflows. Both instruments have an available 96-or 384-well plate reader attached.<\/p>\n<ul>\n<li><a href=\"https:\/\/health.uconn.edu\/flow-cytometry\/wp-content\/uploads\/sites\/123\/2024\/08\/primary-channel-A5.pdf\">Primary Channel<\/a><\/li>\n<\/ul>\n<\/div><\/div><\/div><\/div><div id=\"pg-22-3\"  class=\"panel-grid panel-no-style\" ><div id=\"pgc-22-3-0\"  class=\"panel-grid-cell\" ><div id=\"panel-22-3-0-0\" class=\"so-panel widget widget_widget_sp_image widget_sp_image panel-first-child panel-last-child\" data-index=\"6\" ><img loading=\"lazy\" decoding=\"async\" width=\"385\" height=\"601\" alt=\"BD FACSymphony S6 Cell Sorter\" class=\"attachment-full\" style=\"max-width: 100%;\" srcset=\"https:\/\/health.uconn.edu\/flow-cytometry\/wp-content\/uploads\/sites\/123\/2024\/01\/BD-FACSymphony-S6-Cell-Sorter.jpg 385w, https:\/\/health.uconn.edu\/flow-cytometry\/wp-content\/uploads\/sites\/123\/2024\/01\/BD-FACSymphony-S6-Cell-Sorter-192x300.jpg 192w\" sizes=\"(max-width: 385px) 100vw, 385px\" src=\"https:\/\/health.uconn.edu\/flow-cytometry\/wp-content\/uploads\/sites\/123\/2024\/01\/BD-FACSymphony-S6-Cell-Sorter.jpg\" \/><\/div><\/div><div id=\"pgc-22-3-1\"  class=\"panel-grid-cell\" ><div id=\"panel-22-3-1-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"7\" ><div class=\"textwidget\"><h3>BD FACSymphony S6 Cell Sorter<\/h3>\n<p>The FACSymphony S6 SE has five lasers and 48 algorithmically optimized fluorescence detectors for cellular analysis. This instrument can be used for both conventional and spectral flow cytometry because it uses additional high-sensitivity detectors and new software functionality to detect the full spectrum of emitted light and unmix spectrally live in BD FACS Diva software, all while retaining the flexibility of familiar compensation-based workflows. The sorter is capable of separating 6 distinct cellular populations and has the capacity to sort single cells into 96- or 384-well plates or onto glass slides. The FACSymphony S6 SE cell sorter is installed in a biosafety cabinet and is equipped with an aerosol containment unit to allow sorting of biohazardous materials.<\/p>\n<ul>\n<li><a href=\"https:\/\/health.uconn.edu\/flow-cytometry\/wp-content\/uploads\/sites\/123\/2024\/08\/primary-channel-A5.pdf\">Primary Channel<\/a><\/li>\n<\/ul>\n<\/div><\/div><\/div><\/div><div id=\"pg-22-4\"  class=\"panel-grid panel-no-style\" ><div id=\"pgc-22-4-0\"  class=\"panel-grid-cell\" ><div id=\"panel-22-4-0-0\" class=\"so-panel widget widget_widget_sp_image widget_sp_image panel-first-child panel-last-child\" data-index=\"8\" ><img loading=\"lazy\" decoding=\"async\" width=\"250\" height=\"150\" alt=\"Becton-Dickinson LSR II\" class=\"attachment-full\" style=\"max-width: 100%;\" src=\"https:\/\/health.uconn.edu\/flow-cytometry\/wp-content\/uploads\/sites\/123\/2017\/04\/photo_lsr.jpg\" \/><\/div><\/div><div id=\"pgc-22-4-1\"  class=\"panel-grid-cell\" ><div id=\"panel-22-4-1-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"9\" ><div class=\"textwidget\"><h3>Becton-Dickinson LSR II<\/h3>\n<p>The LSR II-B cell analyzer has five lasers and is able to detect 20 parameters (18 fluorescence plus forward and side scatter). LSR II-B is considered a special order research product (SORP) and was customized for ongoing research at UConn Health.<\/p>\n<ul>\n<li><a href=\"https:\/\/health.uconn.edu\/flow-cytometry\/wp-content\/uploads\/sites\/123\/2017\/04\/lsrII_b.pdf\">LSR II-B Laser Configurations<\/a><\/li>\n<\/ul>\n<\/div><\/div><\/div><\/div><div id=\"pg-22-5\"  class=\"panel-grid panel-no-style\" ><div id=\"pgc-22-5-0\"  class=\"panel-grid-cell\" ><div id=\"panel-22-5-0-0\" class=\"so-panel widget widget_widget_sp_image widget_sp_image panel-first-child panel-last-child\" data-index=\"10\" ><img loading=\"lazy\" decoding=\"async\" width=\"250\" height=\"150\" alt=\"Becton-Dickinson FacsAria II\" class=\"attachment-full\" style=\"max-width: 100%;\" src=\"https:\/\/health.uconn.edu\/flow-cytometry\/wp-content\/uploads\/sites\/123\/2017\/04\/photo_facsaria.jpg\" \/><\/div><\/div><div id=\"pgc-22-5-1\"  class=\"panel-grid-cell\" ><div id=\"panel-22-5-1-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"11\" ><div class=\"textwidget\"><h3>Becton-Dickinson FacsAria II<\/h3>\n<p>The FACS Aria II high-speed cell sorter has five lasers. and can detect up to 18 parameters (16 fluorescence colors plus forward and side scatter). The Aria II cell sorter is installed in a biosafety cabinet and is equipped with an aerosol containment unit to allow sorting of biohazardous materials. Additionally, the Aria II cell sorter is equipped with an automated cell deposition unit (ACDU) that allows for sorting into plates or glass slides. The Aria II sorter is considered a special order research product (SORP) and was customized for ongoing research at UConn Health.<\/p>\n<ul>\n<li><a href=\"https:\/\/health.uconn.edu\/flow-cytometry\/wp-content\/uploads\/sites\/123\/2017\/04\/ariaII_b.pdf\">Aria II-B Laser Configurations<\/a><\/li>\n<\/ul>\n<\/div><\/div><\/div><\/div><div id=\"pg-22-6\"  class=\"panel-grid panel-no-style\" ><div id=\"pgc-22-6-0\"  class=\"panel-grid-cell\" ><div id=\"panel-22-6-0-0\" class=\"so-panel widget widget_widget_sp_image widget_sp_image panel-first-child panel-last-child\" data-index=\"12\" ><img loading=\"lazy\" decoding=\"async\" width=\"470\" height=\"302\" alt=\"BioRad ZE5 Cell Analyzer\" class=\"attachment-full\" style=\"max-width: 100%;\" srcset=\"https:\/\/health.uconn.edu\/flow-cytometry\/wp-content\/uploads\/sites\/123\/2021\/01\/ze5-cell-analyzer-detail.jpg 470w, https:\/\/health.uconn.edu\/flow-cytometry\/wp-content\/uploads\/sites\/123\/2021\/01\/ze5-cell-analyzer-detail-300x193.jpg 300w\" sizes=\"(max-width: 470px) 100vw, 470px\" src=\"https:\/\/health.uconn.edu\/flow-cytometry\/wp-content\/uploads\/sites\/123\/2021\/01\/ze5-cell-analyzer-detail.jpg\" \/><\/div><\/div><div id=\"pgc-22-6-1\"  class=\"panel-grid-cell\" ><div id=\"panel-22-6-1-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"13\" ><div class=\"textwidget\"><h3>BioRad ZE5<\/h3>\n<p>The ZE5 Cell Analyzer has 5 lasers and allows for 27 fluorescence parameter flow cytometry analysis. It integrates a universal plate loader that accepts single tubes, tube racks, or 96- and 384-well plates. It has integrated sample vortexing, sample temperature control, and volumetric cell counting. Forward scatter can be detected from both the 488nm and 405nm lasers which may allow for better detection of small particles.<\/p>\n<ul>\n<li><a href=\"https:\/\/health.uconn.edu\/flow-cytometry\/wp-content\/uploads\/sites\/123\/2021\/01\/ZE5-Reagent-Guide.pdf\">ZE5 Reagent Guide<\/a><\/li>\n<\/ul>\n<\/div><\/div><\/div><\/div><\/div>","protected":false},"excerpt":{"rendered":"<p>Accuri\u2122 C6 Located in room E3004, this simple high throughput system is equipped with a blue and red laser, two light scatter detectors, and four fluorescence detectors with optical filters optimized for the detection of FITC, PE, PerCP, and APC. A compact optical design, fixed alignment, and preoptimized detector settings make the system easier to [&hellip;]<\/p>\n","protected":false},"author":38,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"acf":[],"publishpress_future_action":{"enabled":false,"date":"2026-04-15 00:16:20","action":"change-status","newStatus":"draft","terms":[],"taxonomy":""},"_links":{"self":[{"href":"https:\/\/health.uconn.edu\/flow-cytometry\/wp-json\/wp\/v2\/pages\/22"}],"collection":[{"href":"https:\/\/health.uconn.edu\/flow-cytometry\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/health.uconn.edu\/flow-cytometry\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/health.uconn.edu\/flow-cytometry\/wp-json\/wp\/v2\/users\/38"}],"replies":[{"embeddable":true,"href":"https:\/\/health.uconn.edu\/flow-cytometry\/wp-json\/wp\/v2\/comments?post=22"}],"version-history":[{"count":20,"href":"https:\/\/health.uconn.edu\/flow-cytometry\/wp-json\/wp\/v2\/pages\/22\/revisions"}],"predecessor-version":[{"id":230,"href":"https:\/\/health.uconn.edu\/flow-cytometry\/wp-json\/wp\/v2\/pages\/22\/revisions\/230"}],"wp:attachment":[{"href":"https:\/\/health.uconn.edu\/flow-cytometry\/wp-json\/wp\/v2\/media?parent=22"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}