2024
Effects of intermittent senolytic therapy on bone metabolism in postmenopausal women: a phase 2 randomized controlled trial
Farr JN, Atkinson EJ, Achenbach SJ, Volkman TL, Tweed AJ, Vos SJ, Ruan M, Sfeir J, Drake MT, Saul D, Doolittle ML, Bancos I, Yu K, Tchkonia T, LeBrasseur NK, Kirkland JL, Monroe DG, Khosla S. Nature Medicine. 2024 Sep.
SenNet recommendations for detecting senescent cells in different tissues
Suryadevara V, Hudgins AD, Rajesh A, Pappalardo A, Karpova A, Dey AK, Hertzel A, Agudelo A, Rocha A, Soygur B, Schilling B, Carver CM, Aguayo-Mazzucato C, Baker DJ, Bernlohr DA, Jurk D, Mangarova DB, Quardokus EM, Enninga EAL, Schmidt EL, Chen F, Duncan FE, Cambuli F, Kaur G, Kuchel GA, Lee G, Daldrup-Link HE, Martini H, Phatnani H, Al-Naggar IM, Rahman I, Nie J, Passos JF, Silverstein JC, Campisi J, Wang J, Iwasaki K, Barbosa K, Metis K, Nernekli K, Niedernhofer LJ, Ding L, Wang L, Adams LC, Ruiyang L, Doolittle ML, Teneche MG, Schafer MJ, Xu M, Hajipour M, Boroumand M, Basisty N, Sloan N, Slavov N, Kuksenko O, Robson P, Gomez PT, Vasilikos P, Adams PD, Carapeto P, Zhu Q, Ramasamy R, Perez-Lorenzo R, Fan R, Dong R, Montgomery RR, Shaikh S, Vickovic S, Yin S, Kang S, Suvakov S, Khosla S, Garovic VD, Menon V, Xu Y, Song Y, Suh Y, Dou Z, Neretti N. Nature Reviews Molecular Cell Biology. 2024 Jun.
Osteochondroprogenitor cells and neutrophils expressing p21 and senescence markers modulate fracture repair
Saul D#, Doolittle ML#,*, Rowsey JL, Froemming MN, Kosinsky RL, Vos SJ, Ruan M, LeBrasseur NK, Chandra A, Pignolo RJ, Passos JF, Farr JN, Monroe DG, Khosla S*. Journal of Clinical Investigation. 2024 May. #First authors. *Corresponding authors
Senescent skeletal muscle fibroadipogenic progenitors recruit and promote M2 polarization of macrophages
Zhang X, Ng YE, Chini LCS, Heeren AA, White TA, Li H, Huang H, Doolittle ML, Khosla S, LeBrasseur NK. Aging Cell. 2024 Mar.
2023
Influences of Aged Bone Marrow Macrophages on Skeletal Health and Senescence
Pappert M, Khosla S, Doolittle ML*. Current Osteoporosis Reports. 2023 Dec. *Corresponding Author
Single‐Cell Integration of BMD GWAS Results Prioritize Candidate Genes Influencing Age‐Related Bone Loss
Doolittle ML, Khosla S, Saul D. JBMR Plus. 2023 Oct.
Modest Effects of Osteoclast‐Specific ERα Deletion after Skeletal Maturity
Doolittle ML, Eckhardt BA, Vos SJ, Grain S, Rowsey JL, Ruan M, Saul D, Farr JN, Weivoda MM, Khosla S, Monroe DG. JBMR Plus. 2023 Oct.
Multiparametric senescent cell phenotyping reveals targets of senolytic therapy in the aged murine skeleton
Doolittle ML, Saul D, Kaur J, Rowsey JL, Vos SJ, Pavelko KD, Farr JN, Monroe DG, Khosla S. Nature Communications. 2023 Jul.
MicroRNA 19a 3p Decreases with Age in Mice and Humans and Inhibits Osteoblast Senescence
Kaur J, Saul D, Doolittle ML, Farr JN, Khosla S, Monroe DG. JBMR Plus. 2023 Jun.
In vitro and in vivo effects of zoledronic acid on senescence and senescence-associated secretory phenotype markers
Samakkarnthai P, Saul D, Zhang L, Aversa Z, Doolittle ML, Sfeir JG, Kaur J, Atkinson EJ, Edwards JR, Russell GG, Pignolo RJ, Kirkland JL, Tchkonia T, Niedernhofer LJ, Monroe DG, LeBrasseur NK, Farr JN, Robbins PD, Khosla S. Aging. 2023 May.
Local senolysis in aged mice only partially replicates the benefits of systemic senolysis
Farr JN, Saul D, Doolittle ML, Kaur J, Rowsey JL, Vos SJ, Froemming MN, Lagnado AB, Zhu Y, Ikeno Y, Pignolo RJ, Niedernhofer NJ, Robbins PD, Jurk D, Passos JF, LeBrasseur NK, Tchkonia T, Kirkland JL, Monroe DG, Khosla S. Journal of Clinical Investigation. 2023 Apr.
2022
Skeletal Effects of Inducible ERα Deletion in Osteocytes in Adult Mice
Doolittle ML, Saul D, Kaur J, Rowsey JL, Eckhardt BA, Vos SJ, Kroupova K, Ruan M, Weivoda MM, Oursler MJ, Farr JN, Monroe DG, Khosla S. Journal of Bone and Mineral Research. 2022 Sep.
A new gene set identifies senescent cells and predicts senescence-associated pathways across tissues
Saul D, Kosinsky RL, Atkinson EJ, Doolittle ML, Zhang X, LeBrasseur NK, Pignolo RJ, Robbins PD, Niedernhofer LJ, Ikeno Y, Jurk D, Passos JF, Hickson LJ, Xue A, Monroe DG, Tchkonia T, Kirkland JL, Farr JN, Khosla S. Nature Communications. 2022 Aug.
Identification of a suitable endogenous control miRNA in bone aging and senescence
Kaur J, Saul D, Doolittle ML, Rowsey JL, Vos SJ, Farr JN, Khosla S, Monroe DG. Gene. 2022 Aug.
Targeted clearance of p21‐ but not p16‐positive senescent cells prevents radiation‐induced osteoporosis and increased marrow adiposity
Chandra A, Lagnado AB, Farr JN, Doolittle ML, Tchkonia T, Kirkland JL, LeBrassseur NK, Robbins PD, Niedernhofer LJ, Ikeno Y, Passos JF, Monroe DG, Pignolo RJ, Khosla S. Aging Cell. 2022 Apr.
2021
Modulation of fracture healing by the transient accumulation of senescent cells
Saul D, Monroe DG, Rowsey JL, Kosinsky RL, Vos SJ, Doolittle ML, Farr JN, Khosla S. Elife.2021 Oct.
The role of senolytics in osteoporosis and other skeletal pathologies
Doolittle ML, Monroe DG, Farr JN, Khosla S. Mechanisms of Ageing and Development. 2021 Oct.
Neutrophils induce paracrine telomere dysfunction and senescence in ROS‐dependent manner
Lagnado A, Leslie J, Ruchaud-Sparagano MH, Victorelli S, Hirsova P, Ogrodnik M, Collins AL, Visioli MG, Habiballa L, Saretzki G, Evans SA, Salmonowicz, Hruby A, Geh D, Pavelko KD, Dolan D, Reeves HL, Grellscheid S, Wilson CH, Pandanaboyana S, Doolittle ML, von Zglinicki T, Oakley F, Gallage S, Wilson C, Birch J, Carroll B, Chapman J, Heikenwalder M, Neretti N, Khosla S, Masuda CA, Tchkonia T, Kirkland JL, Jurk D, Mann D, Passos JF. EMBO Journal. 2021 May.
Isolation and Culture of Neonatal Mouse Calvarial Osteoblasts
Doolittle, ML, Ackert-Bicknell, CL, Jonason, JH. Methods in Molecular Biology - Skeletal Development and Repair. 2021.
2020
Osteocyte Cellular Senescence
Farr JN, Kaur J, Doolittle ML, Khosla S. Current Osteoporosis Reports. 2020 Oct.
Genetic analysis of osteoblast activity identifies Zbtb40 as a regulator of osteoblast activity and bone mass
Doolittle ML#, Calabrese GM#, Mesner LD, Maynard RD, Ackert-Bicknell CL*, Farber CR*. PLOS Genetics. 2020 Jun. #First authors. *Corresponding authors.
Pre-2020
Targeting the gut microbiome to treat the osteoarthritis of obesity
Schott EM, Farnsworth CW, Grier A, Lillis JA, Soniwala S, Dadourian GH, Bell RD, Doolittle ML, Villani DA, Awad H, Ketz JP, Kamal F, Ackert-Bicknell C, Ashton JM, Gill SR, Mooney RA, Zuscik MJ. JCI Insight. 2018 Apr.
A Nondestructive, Reproducible Method of Measuring Joint Reaction Force at the Distal Radioulnar Joint
Canham CD, Schreck MJ, Maqsoodi N, Doolittle ML, Olles M, Elfar JC. Journal of Hand Surgery. 2015 Jun.