Direct Immunofluorescence Studies for Mucocutaneous Disorders

Immunofluorescence is a clinically proven test to diagnose cutaneous immunologic disorders such as:

  • Blistering diseases (Bullous Pemphigoid)
  • Connective tissue diseases (Lupus Erythematosus)
  • Vasculitis (Henoch-Schonlein Purpura)

Our Services

  • Fast turnaround time: within 24 to 48 hours after specimen receipt
  • DIF performed on skin, mucosa and ocular tissue in our CLIA-approved laboratory
  • Correlation of DIF results with clinical and histopathological findings
  • Accurate and concise sign-out of cases
  • Photomicrographs of DIF results are available upon request
  • Direct communication with laboratory director, Michael Murphy, M.D., regarding DIF results and other queries: 860-679-3474 or

Sending a Specimen for Direct Immunofluorescence Testing

Submission of Tissue: Place direct immunofluorescence (DIF) tissue specimen in the provided white-top vial containing Zeus solution, and store at room temperature. The submission of additional tissue in formalin fixative is recommended for definitive clinical-histopathological correlation of any skin lesion(s). Complete the UConn Health dermatopathology requisition form, include patient’s insurance details, and mail to the address below. See Biopsy Site Guidelines below for more information.

View more detailed information regarding DIF biopsies:

Direct Immunofluorescence Supplies

The UConn Health Dermatopathology Service provides complimentary DIF bottles containing Zeus solution and requisition forms. Please call the Dermatopathology Laboratory to request supplies at 860-679-3669. DIF solution has a shelf-life of one year.

Send DIF cases to:
Attn: Michael Murphy, M.D.
Dermatopathology Laboratory
Department of Dermatology
UConn Health
21 South Road
Farmington, CT 06030