![\"Bruce](\"https:\/\/health.uconn.edu\/cell-biology\/wp-content\/uploads\/sites\/115\/2017\/10\/photo_white2.jpg\")
Professor\nDepartment of Cell Biology<\/p>\n
Professor
\nDepartment of Cell Biology<\/p>\n
Phone: 860-679-2811 UConn Health We are focused on metabolic reprogramming that accompanies epithelial-mesenchymal transition (EMT) in human breast cancer cell lines. We used 3-dimensional mammosphere culture conditions to induce a stable EMT in the two epithelial MCF7 and BT474 breast cancer cell lines, thereby generating corresponding post-EMT mesenchymal cell lines, termed MCF7M and BT474M cells. We are currently utilizing a 3-dimensional culture system in Matrigel, along with physiological levels of glucose, lactate, glutamine and pyruvate, in order to more accurately mimic the in vivo condition. We recently reported that both epithelial cell lines are more oxidative, and express enzymes involved in reverse glycolysis that potentially redirect carbons to anabolic pathways involved in cell growth. In contrast, the two mesenchymal cell lines proliferate faster and are more motile. The MCF7M and BT474M cells are also significantly more glycolytic, expressing higher levels of GLUT transporters and consuming more glucose, and expressing higher levels of the lactate transporter, MCT4, and producing more lactate than the epithelial cells. We are currently examining how lactate metabolism is shifted in these cell lines, and the potential role of the endogenous lactate transporter, GPR81, in the regulation of lactate metabolism. Most recently, we have also examined how the NAD+\/NADH ratio might be altered by but also control metabolic reprogramming in the epithelial vs mesenchymal cell lines. In this context, we have begun to examine whether the metabolism of ethanol, which is linked to a higher risk for diagnosis of some forms of breast cancer, might impact the NAD+\/NADH ratio and thus metabolic reprogramming, cell proliferation and cell viability.<\/p>\n Kondaveeti, Y., Guttilla Reed, I.K., and White, B.A. (2015). Epithelial\u2013mesenchymal transition induces similar metabolic alterations in two independent breast cancer cell lines<\/a>. Cancer Letters<\/em> 364 (2015) 44\u201358.<\/p>\n Guttilla, IK, Phoenix, KN, Hong, X, Tirnauer, JS, Claffey, KP, and White, BA\u00a0 (2012)\u00a0 Prolonged mammosphere culture of MCF-7 cells induces an EMT and repression of the estrogen receptor by microRNAs<\/a>. Breast Cancer Res Treatment <\/em>132: 75-85.<\/p>\n Gutilla I.K., Adams, B.D., White, B.A. (2012). ER\u03b1, microRNAs, and the epithelial-mesenchymal transition in breast cancer<\/a>. Trends Endocrinol Metab<\/em>. 23(2):73-82.<\/p>\n Guttilla, IK and White, BA (2009)\u00a0 Coordinate regulation of FOXO1 by miR-27a, miR-96, and miR-182 in breast cancer cells<\/a>. J Biol Chem<\/em> 284: 23204\u201323216.<\/p>\n Adams BD, Cowee DM, White BA (2009) The role of miR-206 in the epidermal growth factor (EGF) induced repression of estrogen receptor-a \u00a0(ERa) signaling and a luminal phenotype in MCF-7 breast cancer cells<\/a>. Mol Endocrinol<\/em> 23: 1215-1230. PMCID2718747.<\/p>\n Adams, B, Claffey, KP, White, BA (2009) Argonaute-2 Expression is Regulated By Epidermal Growth Factor Receptorand Mitogen-activated Protein Kinase Signaling and Correlates with a Transformed Phenotype in Breast Cancer Cells<\/a>. Endocrinology<\/em> 150:14-23.<\/p>\n Adams B, Furneaux H, White BA (2007) The micro-RNA miR-206 Targets the Human Estrogen Receptor-\u03b1, Repressing ER\u03b1 Expression and Function in Breast Cancer Cell Lines<\/a>. Mol. Endocrinol<\/em>. 21: 1132-1147.<\/p>\n Guttilla, IK, Adams, BD, White BA (2012) Trends Endocrinol Metab 23 :73-82. ERa, microRNAs, and the epithelial\u2013mesenchymal transition in breast cancer<\/a>.<\/p>\n
\nEmail: bwhite@uchc.edu <\/a>
\nOffice: E5054<\/p>\n
\n263 Farmington Avenue
\nFarmington, CT 06030<\/p>\nResearch Interests<\/h3>\n
Recent Peer-reviewed Research Publications<\/h3>\n
![\"The](\"https:\/\/health.uconn.edu\/cell-biology\/wp-content\/uploads\/sites\/115\/2017\/10\/image_mecover_sm.jpg\")
<\/p>\nRecent Reviews<\/h3>\n