Instructor (In Residence)
Department of Cell Biology
UConn Health, School of Medicine
263 Farmington Avenue, Farmington, CT 06030
- Apply regenerative medicine to develop personalized therapeutic options for hematopoietic diseases.
- Understand the cross talk between human hematopoietic niche cells and hematopoietic cells during hemogenic endothelial to hematopoietic transition using human induced pluripotent stem cells.
In Dr. Oguro’s lab, my research focuses on generating hematopoietic stem cells (HSCs) from human induced pluripotent stem cells (iPSCs). HSC transplantation is widely used in clinics to treat blood disorders and malignancies. However, there is limited availability of suitable donors and current clinical HSC collection protocols do not always produce enough number of HSCs from donors to ensure transplantation success. Thus, there is a strong demand for an alternative source of autologous HSCs for clinical transplantation. A promising source for a virtually unlimited supply of HSCs is the in vitro differentiation of human iPSCs into HSCs. However, the derivation of HSCs from human iPSCs in culture remains elusive. To fill this gap, we are developing methods to generate autologous HSCs from human iPSCs by mimicking the environment during the developmental process of HSCs (Figure 1). Additionally, I am exploring the role of estrogen receptor α signaling in HSC proliferation and mobilization.
JK Kanaujiya, EG Lingenheld, WC Skarnes and H Oguro. Inhibition of P53-mediated apoptosis promotes hematopoietic differentiation of human pluripotent stem cell-derived hemogenic endothelial cells. The International Society of Stem Cell Research (ISSCR) 2020 Virtual Annual Meeting.
J Kanaujiya, J Balsbaugh, E Reichenberger, I Chen. Whole-cell proteomic profiling of osteoclasts from a mouse model for craniometaphyseal dysplasia. The American Society for Bone and Mineral Research (ASBMR) 2018 Annual Meeting at Montréal, Québec, Canada. Journal of Bone and Mineral Research, 33, 183-184.
- Kanaujiya J, Bastow E, Luxmi R, Hao Z, Zattas D, Hochstrasser M, Reichenberger EJ, Chen IP. Rapid degradation of progressive ankylosis protein (ANKH) in craniometaphyseal dysplasia. Sci Rep. 2018 Oct 24;8(1):15710. doi: 10.1038/s41598-018-34157-5. PubMed PMID: 30356088; PubMed Central PMCID: PMC6200807
- Chen IP, Luxmi R, Kanaujiya J, Hao Z, Reichenberger EJ. Craniometaphyseal Dysplasia Mutations in ANKH Negatively Affect Human Induced Pluripotent Stem Cell Differentiation into Osteoclasts. Stem Cell Reports. 2017 Nov 14;9(5):1369-1376. doi: 10.1016/j.stemcr.2017.09.016. Epub 2017 Oct 19. PubMed PMID: 29056330; PubMed Central PMCID: PMC5830990.
- Kanaujiya JK, Lochab S, Kapoor I, Pal P, Datta D, Bhatt ML, Sanyal S, Behre G, Trivedi AK. Proteomic identification of Profilin1 as a corepressor of estrogen receptor alpha in MCF7 breast cancer cells. Proteomics. 2013 Jul;13(14):2100-12. doi: 10.1002/pmic.201200534. Epub 2013 Jun 12. PubMed PMID: 23576398.
- Pal P, Kanaujiya JK, Lochab S, Tripathi SB, Bhatt ML, Singh PK, Sanyal S, Trivedi AK. 2-D gel electrophoresis-based proteomic analysis reveals that ormeloxifen induces G0-G1 growth arrest and ERK-mediated apoptosis in chronic myeloid leukemia cells K562. Proteomics. 2011 Apr;11(8):1517-29. doi: 10.1002/pmic.201000720. Epub 2011 Feb 25. PubMed PMID: 21360677.