{"id":4268,"date":"2024-07-29T12:44:03","date_gmt":"2024-07-29T16:44:03","guid":{"rendered":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/?page_id=4268"},"modified":"2026-04-10T16:35:26","modified_gmt":"2026-04-10T20:35:26","slug":"background-content","status":"publish","type":"page","link":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/background-content\/","title":{"rendered":"Background Content"},"content":{"rendered":"<h2>Brief Background on Digital Imaging<\/h2>\n<p>The first video presents a brief introduction to the concept of digital images, such as those collected on the Zeiss confocal microscopes. (<a href=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2024\/07\/IntroDigtialImagesCC2.mp4\" target=\"_blank\" rel=\"noopener\">Accessible version with closed captions<\/a>.)<\/p>\n<div style=\"width: 640px;\" class=\"wp-video\"><!--[if lt IE 9]><script>document.createElement('video');<\/script><![endif]-->\n<video class=\"wp-video-shortcode\" id=\"video-4268-1\" width=\"640\" height=\"360\" poster=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2024\/07\/IntroDigtialImages_First_Frame.png\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2024\/07\/IntroDigtialImages.mp4?_=1\" \/><a href=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2024\/07\/IntroDigtialImages.mp4\">https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2024\/07\/IntroDigtialImages.mp4<\/a><\/video><\/div>\n<p class=\"sectiontop-spacing\">The following video discusses how you should capture your digital images on the Zeiss confocal, while considering the Nyquist Theorem. (<a href=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2024\/08\/DigitalImageNyquistCC.mp4\" target=\"_blank\" rel=\"noopener\">Accessible version with closed captions<\/a>)<\/p>\n<div style=\"width: 640px;\" class=\"wp-video\"><video class=\"wp-video-shortcode\" id=\"video-4268-2\" width=\"640\" height=\"360\" poster=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2024\/08\/DigitalImageNyquist_First_Frame.png\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2024\/08\/DigitalImageNyquist.mp4?_=2\" \/><a href=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2024\/08\/DigitalImageNyquist.mp4\">https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2024\/08\/DigitalImageNyquist.mp4<\/a><\/video><\/div>\n<h2 class=\"sectiontop-spacinglarge\">Things to Consider When Taking a Z-Stack<\/h2>\n<p>The video reviews the importance of using Nyquist sampling and avoiding saturation when collecting your z-stacks on the confocal microscope.(<a href=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2026\/04\/Z-stacks-CC.mp4\">Accessible version with closed captions<\/a>)<\/p>\n<div style=\"width: 640px;\" class=\"wp-video\"><video class=\"wp-video-shortcode\" id=\"video-4268-3\" width=\"640\" height=\"361\" poster=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2026\/04\/Z_stacks_First_Frame.png\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2026\/04\/Z-stacks.mp4?_=3\" \/><a href=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2026\/04\/Z-stacks.mp4\">https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2026\/04\/Z-stacks.mp4<\/a><\/video><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Brief Background on Digital Imaging The first video presents a brief introduction to the concept of digital images, such as those collected on the Zeiss confocal microscopes. (Accessible version with closed captions.) The following video discusses how you should capture your digital images on the Zeiss confocal, while considering the Nyquist Theorem. (Accessible version with [&hellip;]<\/p>\n","protected":false},"author":283,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"acf":[],"publishpress_future_action":{"enabled":false,"date":"2026-05-07 00:25:00","action":"change-status","newStatus":"draft","terms":[],"taxonomy":""},"_links":{"self":[{"href":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-json\/wp\/v2\/pages\/4268"}],"collection":[{"href":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-json\/wp\/v2\/users\/283"}],"replies":[{"embeddable":true,"href":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-json\/wp\/v2\/comments?post=4268"}],"version-history":[{"count":17,"href":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-json\/wp\/v2\/pages\/4268\/revisions"}],"predecessor-version":[{"id":5135,"href":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-json\/wp\/v2\/pages\/4268\/revisions\/5135"}],"wp:attachment":[{"href":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-json\/wp\/v2\/media?parent=4268"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}