{"id":3963,"date":"2023-11-02T13:06:51","date_gmt":"2023-11-02T17:06:51","guid":{"rendered":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/?page_id=3963"},"modified":"2026-02-10T11:06:59","modified_gmt":"2026-02-10T16:06:59","slug":"live-cell-imaging","status":"publish","type":"page","link":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/live-cell-imaging\/","title":{"rendered":"Live Cell Imaging"},"content":{"rendered":"

Prokaryotic and eukaryotic naturally exhibit autofluorescence due to the cellular components and metabolites, such as flavins, NADH, lipofuscins, collagen, and elastin. Culture media can also interfere with fluorescent imaging due to phenol red and additional metabolites. Please review the following content below to find something suitable for your experiments.<\/p>\n

Media<\/h2>\n

Bogdanov, A., Bogdanova, E., Chudakov, D.\u00a0<\/span>et al.<\/i>\u00a0Cell culture medium affects GFP photostability: a solution.\u00a0<\/span>Nat Methods<\/i>\u00a0<\/span>6<\/b>, 859\u2013860 (2009). https:\/\/doi.org\/10.1038\/nmeth1209-859<\/span> (opens in a new tab)<\/span><\/a><\/p>\n

ThermoFisher Scientific - FluoroBrite DMEM Media (opens in a new tab)<\/span><\/a><\/p>\n

Dulbecco's MEM (opens in a new tab)<\/span><\/a> - (USBiological Life Sciences (opens in a new tab)<\/span><\/a>) It is recommended to make a stock that is buffered with NaHCO3 for 5% CO2 and supplement the medium with 2% FBS. It has been found that HEPES is sensitive to light and produces hydrogen peroxide as referenced from Analysis of the cytotoxic effects of light-exposed HEPES-containing culture medium (opens in a new tab)<\/span><\/a>.<\/p>\n

Antibleaching live cell visualization medium\u00a0<\/span>DMEMgfp<\/sup>-2 (opens in a new tab)<\/span><\/a> - cell culture medium for cells expressing GFP. Please note references, Cell culture medium affects GFP photostability: a solution (opens in a new tab)<\/span><\/a>,\u00a0 and\u00a0<\/span>Anti-fading media for live cell GFP imaging (opens in a new tab)<\/span><\/a>.<\/p>\n

FluoroBrite DMEM (opens in a new tab)<\/span><\/a> - designed to enhance the signal-to-noise ratio of fluorophores without the background fluorescence from phenol red. Can be used during live cell imaging to maintain cell health.<\/p>\n

 <\/p>\n

Supplies<\/h2>\n

Cellvis (opens in a new tab)<\/span><\/a> - Glass Bottom Dishes<\/p>\n

Fisher Scientific - Thermo Scientific Nunc Glass Bottom Dishes (opens in a new tab)<\/span><\/a> and Thermo Scientific Nunc Lab-Tek and Lab Tek II Chamber Slides (opens in a new tab)<\/span><\/a><\/p>\n

Ibidi (opens in a new tab)<\/span><\/a> - Chambered Coverslips, and Culture Inserts (opens in a new tab)<\/span><\/a><\/p>\n

Mattek (opens in a new tab)<\/span><\/a> - Glass Bottom Dishes<\/p>\n

Nunc Lab (opens in a new tab)<\/span><\/a>-Tek II Chambered Coverglass<\/p>\n

Wilco (opens in a new tab)<\/span><\/a> - Glass Bottom Dishes<\/p>\n

 <\/p>\n<\/div><\/div><\/div><\/div><\/div>","protected":false},"excerpt":{"rendered":"

Prokaryotic and eukaryotic naturally exhibit autofluorescence due to the cellular components and metabolites, such as flavins, NADH, lipofuscins, collagen, and elastin. Culture media can also interfere with fluorescent imaging due to phenol red and additional metabolites. Please review the following content below to find something suitable for your experiments. Media Bogdanov, A., Bogdanova, E., Chudakov, […]<\/p>\n","protected":false},"author":283,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"acf":[],"publishpress_future_action":{"enabled":false,"date":"2026-04-20 09:47:49","action":"change-status","newStatus":"draft","terms":[],"taxonomy":""},"_links":{"self":[{"href":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-json\/wp\/v2\/pages\/3963"}],"collection":[{"href":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-json\/wp\/v2\/users\/283"}],"replies":[{"embeddable":true,"href":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-json\/wp\/v2\/comments?post=3963"}],"version-history":[{"count":18,"href":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-json\/wp\/v2\/pages\/3963\/revisions"}],"predecessor-version":[{"id":5078,"href":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-json\/wp\/v2\/pages\/3963\/revisions\/5078"}],"wp:attachment":[{"href":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-json\/wp\/v2\/media?parent=3963"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}