{"id":3830,"date":"2023-07-26T11:45:42","date_gmt":"2023-07-26T15:45:42","guid":{"rendered":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/?page_id=3830"},"modified":"2026-02-11T15:15:39","modified_gmt":"2026-02-11T20:15:39","slug":"super-resolution","status":"publish","type":"page","link":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/super-resolution\/","title":{"rendered":"Super Resolution"},"content":{"rendered":"<div id=\"pl-3830\"  class=\"panel-layout\" ><div id=\"pg-3830-0\"  class=\"panel-grid panel-no-style\" ><div id=\"pgc-3830-0-0\"  class=\"panel-grid-cell\" ><div id=\"panel-3830-0-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child\" data-index=\"0\" ><div class=\"textwidget\"><p><div id=\"metaslider-id-3878\" style=\"max-width: 350px; margin: 0 auto;\" class=\"ml-slider-3-80-0 ml-slider-pro-2-36-0 metaslider metaslider-flex metaslider-3878 ml-slider ms-theme-architekt has-dots-nav\" role=\"region\" aria-roledescription=\"Slideshow\" aria-label=\"Super Resolution Microscopy vs Widefield Imaging\">\n    <div id=\"metaslider_container_3878\">\n        <div id=\"metaslider_3878\" class=\"flexslider\">\n            <ul class='slides'>\n                <li style=\"display: block; width: 100%;\" class=\"slide-3886 ms-image \" aria-roledescription=\"slide\" aria-label=\"slide-3886\"><img loading=\"lazy\" decoding=\"async\" width=\"700\" height=\"700\" src=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/08\/SAureus-WF-Zoomed.png\" class=\"slider-3878 slide-3886\" alt=\"SAureus WF Nile Red\" rel=\"\" title=\"\" srcset=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/08\/SAureus-WF-Zoomed.png 700w, https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/08\/SAureus-WF-Zoomed-300x300.png 300w, https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/08\/SAureus-WF-Zoomed-150x150.png 150w\" sizes=\"(max-width: 700px) 100vw, 700px\" \/><div class=\"caption-wrap\"><div class=\"caption\">Zoomed wide field fluorescent microscopy image of S. aureus stained with Nile Red. (J.Batchelder, Mok Lab)<\/div><\/div><\/li>\n                <li style=\"display: none; width: 100%;\" class=\"slide-3888 ms-image \" aria-roledescription=\"slide\" aria-label=\"slide-3888\"><img loading=\"lazy\" decoding=\"async\" width=\"700\" height=\"700\" src=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/08\/SAureus-SIM-Zoomed.png\" class=\"slider-3878 slide-3888\" alt=\"SAureus SIM Nile Red\" rel=\"\" title=\"\" srcset=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/08\/SAureus-SIM-Zoomed.png 700w, https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/08\/SAureus-SIM-Zoomed-300x300.png 300w, https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/08\/SAureus-SIM-Zoomed-150x150.png 150w\" sizes=\"(max-width: 700px) 100vw, 700px\" \/><div class=\"caption-wrap\"><div class=\"caption\">Zoomed SIM image of  S. aureus stained with Nile Red. (J.Batchelder, Mok Lab)<\/div><\/div><\/li>\n                <li style=\"display: none; width: 100%;\" class=\"slide-3898 ms-image \" aria-roledescription=\"slide\" aria-label=\"slide-3898\"><img loading=\"lazy\" decoding=\"async\" width=\"700\" height=\"700\" src=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/08\/MT-13-WF-Single-Plane.png\" class=\"slider-3878 slide-3898\" alt=\"Widefield Image MitoTracker\" rel=\"\" title=\"\" srcset=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/08\/MT-13-WF-Single-Plane.png 700w, https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/08\/MT-13-WF-Single-Plane-300x300.png 300w, https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/08\/MT-13-WF-Single-Plane-150x150.png 150w\" sizes=\"(max-width: 700px) 100vw, 700px\" \/><div class=\"caption-wrap\"><div class=\"caption\">Widefield microscope image of mitochondria. (CCAM)<\/div><\/div><\/li>\n                <li style=\"display: none; width: 100%;\" class=\"slide-3899 ms-image \" aria-roledescription=\"slide\" aria-label=\"slide-3899\"><img loading=\"lazy\" decoding=\"async\" width=\"700\" height=\"700\" src=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/08\/MT-13-SIM-Single-Plane.png\" class=\"slider-3878 slide-3899\" alt=\"SIM Image MitoTracker\" rel=\"\" title=\"\" srcset=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/08\/MT-13-SIM-Single-Plane.png 700w, https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/08\/MT-13-SIM-Single-Plane-300x300.png 300w, https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/08\/MT-13-SIM-Single-Plane-150x150.png 150w\" sizes=\"(max-width: 700px) 100vw, 700px\" \/><div class=\"caption-wrap\"><div class=\"caption\">SIM image of mitochondria. (CCAM)<\/div><\/div><\/li>\n                <li style=\"display: none; width: 100%;\" class=\"slide-3900 ms-image \" aria-roledescription=\"slide\" aria-label=\"slide-3900\"><img loading=\"lazy\" decoding=\"async\" width=\"700\" height=\"700\" src=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/08\/MT-3D-Scale.png\" class=\"slider-3878 slide-3900\" alt=\"Widefield 3D Stack MitoTracker\" rel=\"\" title=\"\" srcset=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/08\/MT-3D-Scale.png 700w, https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/08\/MT-3D-Scale-300x300.png 300w, https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/08\/MT-3D-Scale-150x150.png 150w\" sizes=\"(max-width: 700px) 100vw, 700px\" \/><div class=\"caption-wrap\"><div class=\"caption\">Widefield microscope 3D stack of mitochondria.  (CCAM)<\/div><\/div><\/li>\n                <li style=\"display: none; width: 100%;\" class=\"slide-3901 ms-image \" aria-roledescription=\"slide\" aria-label=\"slide-3901\"><img loading=\"lazy\" decoding=\"async\" width=\"700\" height=\"700\" src=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/08\/MT-Sim-3D-Scale.png\" class=\"slider-3878 slide-3901\" alt=\"SIM MitoTracker 3D Stack\" rel=\"\" title=\"\" srcset=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/08\/MT-Sim-3D-Scale.png 700w, https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/08\/MT-Sim-3D-Scale-300x300.png 300w, https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/08\/MT-Sim-3D-Scale-150x150.png 150w\" sizes=\"(max-width: 700px) 100vw, 700px\" \/><div class=\"caption-wrap\"><div class=\"caption\">SIM 3D stack of mitochondria.  (CCAM)<\/div><\/div><\/li>\n                <li style=\"display: none; width: 100%;\" class=\"slide-3906 ms-image \" aria-roledescription=\"slide\" aria-label=\"slide-3906\"><img loading=\"lazy\" decoding=\"async\" width=\"700\" height=\"700\" src=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/08\/WF-MAP2-Zoomed.png\" class=\"slider-3878 slide-3906\" alt=\"Zoomed Widefield Image of MAP2 Stained Neurons\" rel=\"\" title=\"\" srcset=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/08\/WF-MAP2-Zoomed.png 700w, https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/08\/WF-MAP2-Zoomed-300x300.png 300w, https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/08\/WF-MAP2-Zoomed-150x150.png 150w\" sizes=\"(max-width: 700px) 100vw, 700px\" \/><div class=\"caption-wrap\"><div class=\"caption\">Zoomed Widefield Image of MAP2 Stained  Neuronal cultures. (K. Caro, Martinelli Lab)<\/div><\/div><\/li>\n                <li style=\"display: none; width: 100%;\" class=\"slide-3907 ms-image \" aria-roledescription=\"slide\" aria-label=\"slide-3907\"><img loading=\"lazy\" decoding=\"async\" width=\"700\" height=\"700\" src=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/08\/STORM-MAP2-GrayAdj.png\" class=\"slider-3878 slide-3907\" alt=\"STORM Imge of MAP2 Stained Dendrites\" rel=\"\" title=\"STORM MAP2 GrayAdj\" srcset=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/08\/STORM-MAP2-GrayAdj.png 700w, https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/08\/STORM-MAP2-GrayAdj-300x300.png 300w, https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/08\/STORM-MAP2-GrayAdj-150x150.png 150w\" sizes=\"(max-width: 700px) 100vw, 700px\" \/><div class=\"caption-wrap\"><div class=\"caption\">STORM Image of MAP2 Stained Dendrites. (K. Caro, Martinelli Lab)<\/div><\/div><\/li>\n            <\/ul>\n        <\/div>\n        \n    <\/div>\n<\/div><\/p>\n<table style=\"border-collapse: collapse; width: 100%;\">\n<caption class=\"caption-larger-trainingvideos\">Super Resolution Techniques<\/caption>\n<thead>\n<tr>\n<th scope=\"col\">Technique<\/th>\n<th scope=\"col\">System<\/th>\n<th scope=\"col\">Lateral Resolution<\/th>\n<th scope=\"col\">Axial Resolution<\/th>\n<th scope=\"col\">Fluorophores<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td style=\"width: 16.6667%; text-align: center;\">SIM<\/td>\n<td style=\"width: 16.6667%; text-align: center;\">Widefield<\/td>\n<td style=\"width: 16.6667%; text-align: center;\">~120 nm<\/td>\n<td style=\"width: 16.6667%; text-align: center;\">~ 250 nm<\/td>\n<td style=\"width: 16.6667%; text-align: center;\">Standard<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 16.6667%; text-align: center;\">SMLM<\/td>\n<td style=\"width: 16.6667%; text-align: center;\">Widefield<\/td>\n<td style=\"width: 16.6667%; text-align: center;\">~20 nm<\/td>\n<td style=\"width: 16.6667%; text-align: center;\">~20 nm<\/td>\n<td style=\"width: 16.6667%; text-align: center;\">Photoswitchable, Photoactivatable<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 16.6667%; text-align: center;\">STED<\/td>\n<td style=\"width: 16.6667%; text-align: center;\">Confocal<\/td>\n<td style=\"width: 16.6667%; text-align: center;\">~20 nm<\/td>\n<td style=\"width: 16.6667%; text-align: center;\">~50 nm<\/td>\n<td style=\"width: 16.6667%; text-align: center;\">Standard<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>SIM - Structured illumination microscopy<br \/>\nSMLM - Single-molecule localization microscopy:<\/p>\n<ul>\n<li>PALM - P<span>hotoactivated localization microscopy<\/span><\/li>\n<li>STORM - Stochastic optical reconstruction microscopy<\/li>\n<li>PAINT - Point accumulation in nanoscale topography<\/li>\n<\/ul>\n<p>STED - Stimulated emission depletion<\/p>\n<\/div><\/div><div id=\"panel-3830-0-0-1\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce\" data-index=\"1\" ><div class=\"textwidget\"><h2>Sample Preparation<\/h2>\n<p><a href=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/07\/Elyra_7_Sample-prep-superresolution-EN_41_011_065.pdf\" target=\"_blank\" rel=\"noopener\">Zeiss Elyra 7 Quick Guide <span class=\"sr-only\"> (opens in a new tab)<\/span><\/a> - Information pertaining to sample preparations for SIM, PALM, and dSTORM.\n<\/p>\n<p class=\"heading-title\"><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/27391487\/\" target=\"_blank\" rel=\"noopener\">Optimizing Imaging Conditions for Demanding Multi-Color Super Resolution Localization Microscopy<\/a> - article introducing a new SMLM imaging buffer, OxEA, for use in 3-color experiments using Alexa dyes 488, 555 and 647.<\/p>\n<\/div><\/div><div id=\"panel-3830-0-0-2\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce\" data-index=\"2\" ><div class=\"textwidget\"><h2>Dyes for Single Molecule Localization<\/h2>\n<p class=\"heading-title\"><a href=\"https:\/\/abberior.rocks\/dyes-labels\/abberior-cage\/\" target=\"_blank\" rel=\"noopener\">abberior CAGE dyes <span class=\"sr-only\"> (opens in a new tab)<\/span><\/a> - family of dyes specific for PALM and STORM.<\/p>\n<\/div><\/div><div id=\"panel-3830-0-0-3\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce\" data-index=\"3\" ><div class=\"textwidget\"><h2>Calibration Tools<\/h2>\n<p class=\"heading-title\"><a href=\"https:\/\/www.gattaquant.com\/products\/gatta-sim-nanoruler.html\" target=\"_blank\" rel=\"noopener\">GATTA quant <span class=\"sr-only\"> (opens in a new tab)<\/span><\/a> - Nanorulers for checking resolution on a SIM system.<\/p>\n<\/div><\/div><div id=\"panel-3830-0-0-4\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-last-child\" data-index=\"4\" ><div class=\"textwidget\"><h2>Reference Articles<\/h2>\n<p class=\"heading-title\"><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/28406496\/\" target=\"_blank\" rel=\"noopener\">Strategic and practical guidelines for successful structured illumination microscopy <span class=\"sr-only\"> (opens in a new tab)<\/span><\/a><\/p>\n<p><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32830013\/\" target=\"_blank\" rel=\"noopener\">Quantitative Data Analysis in Single-Molecule Localization Microscopy <span class=\"sr-only\"> (opens in a new tab)<\/span><\/a><\/p>\n<\/div><\/div><\/div><\/div><\/div>","protected":false},"excerpt":{"rendered":"<p>Super Resolution Techniques Technique System Lateral Resolution Axial Resolution Fluorophores SIM Widefield ~120 nm ~ 250 nm Standard SMLM Widefield ~20 nm ~20 nm Photoswitchable, Photoactivatable STED Confocal ~20 nm ~50 nm Standard SIM &#8211; Structured illumination microscopy SMLM &#8211; Single-molecule localization microscopy: PALM &#8211; Photoactivated localization microscopy STORM &#8211; Stochastic optical reconstruction microscopy PAINT [&hellip;]<\/p>\n","protected":false},"author":283,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"acf":[],"publishpress_future_action":{"enabled":false,"date":"2026-04-11 10:47:48","action":"change-status","newStatus":"draft","terms":[],"taxonomy":""},"_links":{"self":[{"href":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-json\/wp\/v2\/pages\/3830"}],"collection":[{"href":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-json\/wp\/v2\/users\/283"}],"replies":[{"embeddable":true,"href":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-json\/wp\/v2\/comments?post=3830"}],"version-history":[{"count":22,"href":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-json\/wp\/v2\/pages\/3830\/revisions"}],"predecessor-version":[{"id":3848,"href":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-json\/wp\/v2\/pages\/3830\/revisions\/3848"}],"wp:attachment":[{"href":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-json\/wp\/v2\/media?parent=3830"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}