{"id":3732,"date":"2023-05-10T11:33:47","date_gmt":"2023-05-10T15:33:47","guid":{"rendered":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/?page_id=3732"},"modified":"2026-02-06T12:31:34","modified_gmt":"2026-02-06T17:31:34","slug":"general-resources","status":"publish","type":"page","link":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/general-resources\/","title":{"rendered":"General Microscopy Information"},"content":{"rendered":"

Microscopy Techniques<\/h2>\n\n\n\n\n\n\n\n\n\n
\n Comparison of microscopy techniques by lateral, and axial resolution,
\n imaging platform, and fluorochrome requirements.
\n <\/caption>\n
Technique<\/th>\nLateral Resolution(nm)<\/th>\nAxial Resolution(nm)<\/th>\nPlatform<\/th>\nFluorochrome<\/th>\n<\/tr>\n
Widefield<\/td>\n~200-300<\/td>\n~500-700<\/td>\nWidefield<\/td>\nStandard<\/td>\n<\/tr>\n
CLSM<\/td>\n~150-220<\/td>\n~360-500<\/td>\nConfocal<\/td>\nStandard<\/td>\n<\/tr>\n
SIM<\/td>\n~100-120<\/td>\n~350-400<\/td>\nWidefield<\/td>\nStandard<\/td>\n<\/tr>\n
STED<\/td>\n~70-90<\/td>\n~100-200<\/td>\nConfocal<\/td>\nPhotostable<\/td>\n<\/tr>\n
PALM<\/td>\n~20-50<\/td>\n~10-70<\/td>\nWidefield<\/td>\nSwitchable<\/td>\n<\/tr>\n
Note: <\/strong>STED – lateral and axial resolutions of ~ 50nm and ~90nm can be achieved when using dyes resistant to photobleaching.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

(Table adapted from: Kubalov\u00e1 I, N\u011bme\u010dkov\u00e1 A, Weisshart K, H\u0159ibov\u00e1 E, Schubert V. Comparing Super-Resolution Microscopy Techniques to Analyze Chromosomes. Int J Mol Sci. 2021 Feb 14;22(4):1903. doi: 10.3390\/ijms22041903. PMID: 33672992; PMCID: PMC7917581.)
\n<\/em><\/p>\n

Background Information on Microscopy<\/h2>\n

iBiology (opens in a new tab)<\/span><\/a> – presents a series of short videos on light microscopy.
\nMolecular Expressions (opens in a new tab)<\/span><\/a> – Exploring the world of optics and microscopy.
\nEducation in Microscopy and Digital Imaging (opens in a new tab)<\/span><\/a> – Presented by Zeiss, a series of articles and interactive pages from basic to advanced topics in microscopy.
\nMicroscopy U (opens in a new tab)<\/span><\/a> – The source for microscopy education.<\/p>\n

Cell Analysis Resource Center<\/h2>\n

ThermoFisher Scientific (opens in a new tab)<\/span><\/a> – Resource for basic principles about cell analysis, applications, and techniques.<\/p>\n

 <\/p>\n

Transmitted Light Techniques<\/h2>\n

K\u00f6hler Illumination in Brightfield Microscopy (opens in a new tab)<\/span><\/a> – description of the alignment technique as presented by ibidi.<\/p>\n

The Principle of Phase Contrast Microscopy (opens in a new tab)<\/span><\/a> – detailed explanation of phase contrasted, presented by ibidi.<\/p>\n","protected":false},"excerpt":{"rendered":"

Microscopy Techniques Comparison of microscopy techniques by lateral, and axial resolution, imaging platform, and fluorochrome requirements. Technique Lateral Resolution(nm) Axial Resolution(nm) Platform Fluorochrome Widefield ~200-300 ~500-700 Widefield Standard CLSM ~150-220 ~360-500 Confocal Standard SIM ~100-120 ~350-400 Widefield Standard STED ~70-90 ~100-200 Confocal Photostable PALM ~20-50 ~10-70 Widefield Switchable Note: STED – lateral and axial resolutions […]<\/p>\n","protected":false},"author":283,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"acf":[],"publishpress_future_action":{"enabled":false,"date":"2026-04-29 01:40:28","action":"change-status","newStatus":"draft","terms":[],"taxonomy":""},"_links":{"self":[{"href":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-json\/wp\/v2\/pages\/3732"}],"collection":[{"href":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-json\/wp\/v2\/users\/283"}],"replies":[{"embeddable":true,"href":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-json\/wp\/v2\/comments?post=3732"}],"version-history":[{"count":18,"href":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-json\/wp\/v2\/pages\/3732\/revisions"}],"predecessor-version":[{"id":5067,"href":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-json\/wp\/v2\/pages\/3732\/revisions\/5067"}],"wp:attachment":[{"href":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-json\/wp\/v2\/media?parent=3732"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}