{"id":287,"date":"2016-02-02T13:11:42","date_gmt":"2016-02-02T18:11:42","guid":{"rendered":"http:\/\/ccamwp.uh.uconn.edu\/?page_id=287"},"modified":"2026-02-04T16:29:54","modified_gmt":"2026-02-04T21:29:54","slug":"ccam-microscopy-equipment","status":"publish","type":"page","link":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/ccam-microscopy-equipment\/","title":{"rendered":"Microscopy Equipment"},"content":{"rendered":"<div id=\"pl-287\" class=\"panel-layout\">\n<div id=\"pg-287-0\" class=\"panel-grid panel-no-style\">\n<div id=\"pgc-287-0-0\" class=\"panel-grid-cell\" data-weight=\"0.5\">\n<div id=\"panel-287-0-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"0\" data-style=\"{&quot;background_display&quot;:&quot;tile&quot;,&quot;accordion&quot;:&quot;&quot;}\">\n<div class=\"textwidget\">\n<h2 id=\"cellbiology\">Academic Research Building (ARB)<br \/>\nE6011<br \/>\n263 Farmington Avenue<\/h2>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"pg-287-1\" class=\"panel-grid panel-no-style\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;,&quot;full_height&quot;:&quot;&quot;,&quot;cell_alignment&quot;:&quot;flex-start&quot;}\">\n<div id=\"pgc-287-1-0\" class=\"panel-grid-cell\" data-weight=\"1\">\n<div id=\"panel-287-1-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"2\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;,&quot;accordion&quot;:&quot;&quot;}\">\n<div class=\"textwidget\">\n<p>Please refer to the following spectral tools below to see if your probes are compatible with our equipment. If you need further assistance, please contact Susan, <a href=\"mailto:staurovsky@uchc.edu\">staurovsky@uchc.edu<\/a>, <a href=\"tel:18606794686\">860-679-4686<\/a>.<\/p>\n<ul>\n<li>ThermoFisher Scientific <a href=\"https:\/\/www.thermofisher.com\/order\/fluorescence-spectraviewer#!\/\" rel=\"noopener\" target=\"_blank\">Fluorescence SpecrtraViewer (opens in new tab)<\/a><\/li>\n<li>BioLegend <a href=\"https:\/\/www.biolegend.com\/en-us\/spectra-analyzer\" target=\"_blank\" rel=\"noopener\">Fluorescence Spectra Analyzer (opens in new tab)<\/a><\/li>\n<li>FPbase <a href=\"https:\/\/www.fpbase.org\/spectra\/\" target=\"_blank\" rel=\"noopener\">Spectra Viewer (opens in new tab)<\/a><\/li>\n<li>AAT Bioquest <a href=\"https:\/\/www.aatbio.com\/fluorescence-excitation-emission-spectrum-graph-viewer\/\" target=\"_blank\" rel=\"noopener\">Fluorescence Spectrum Viewer (opens in new tab)<\/a><\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"pg-287-2\" class=\"panel-grid panel-no-style\" data-style=\"{&quot;background_display&quot;:&quot;tile&quot;,&quot;full_height&quot;:&quot;&quot;}\">\n<div id=\"pgc-287-2-0\" class=\"panel-grid-cell\" data-weight=\"1\">\n<div id=\"panel-287-2-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"3\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;,&quot;background_image_size&quot;:&quot;full&quot;,&quot;background_image_opacity&quot;:&quot;100&quot;,&quot;border_thickness&quot;:&quot;1px&quot;,&quot;accordion&quot;:&quot;&quot;}\">\n<div class=\"textwidget\">\n<div class=\"panel panel-default\">\n<div class=\"panel-heading\">\n<h3 class=\"panel-title\"><strong>Zeiss LSM\u00a0880<\/strong><\/h3>\n<\/div>\n<div class=\"panel-body\">\n<p><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/07\/880-LSM-WebImg.png\" alt=\"Zeiss LSM 880\" width=\"180\" height=\"190\" class=\"alignleft size-full wp-image-3771\" \/><\/p>\n<ul>\n<li>Mounted on an Axio Observer Z1 with automated XYZ control<\/li>\n<li>Spectral 32-channel QUASAR GaAsp detector, detection range 410 &#8211; 696 nm<\/li>\n<li>Two PMT&#8217;s, detection range 415 &#8211; 735 nm, and a transmitted light detector<\/li>\n<li>Incubation system with temperature, humidity and carbon dioxide control.<\/li>\n<\/ul>\n<table style=\"height: 166px;width: 752px;margin-left: auto;margin-right: auto\">\n<caption>Zeiss LSM 880 Configuration<\/caption>\n<tbody>\n<tr>\n<th scope=\"col\" style=\"width: 306px;text-align: center\">Laser Wavelengths (nm)<\/th>\n<th scope=\"col\" style=\"width: 432px;text-align: center\">Type<\/th>\n<th scope=\"col\" style=\"width: 432px;text-align: center\">Common Fluorophores<\/th>\n<\/tr>\n<tr>\n<td style=\"width: 306px;text-align: center\">\u00a0405 nm<\/td>\n<td style=\"width: 432px;text-align: center\">\u00a0Diode<\/td>\n<td style=\"width: 432px;text-align: center\"><span>Alexa405, DAPI, Hoechst<\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 306px;text-align: center\">\u00a0458, 488, and 514 nm<\/td>\n<td style=\"width: 432px;text-align: center\">Argon<\/td>\n<td style=\"width: 432px;text-align: center\"><span>Alexa488, GFP<\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 306px;text-align: center\">\u00a0561 nm<\/td>\n<td style=\"width: 432px;text-align: center\">\u00a0Diode Pumped Solid State (DPSS)<\/td>\n<td style=\"width: 432px;text-align: center\"><span>Alexa568, Rhodamine<\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 306px;text-align: center\">\u00a0633 nm<\/td>\n<td style=\"width: 432px;text-align: center\">Helium Neon (HeNe)<\/td>\n<td style=\"width: 432px;text-align: center\">Alexa 647, Cy5<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<table style=\"height: 166px;width: 752px;margin-left: auto;margin-right: auto\">\n<caption>Ojectives Available on Zeiss LSM 880<\/caption>\n<tbody>\n<tr>\n<th scope=\"col\" style=\"width: 188px;text-align: center\">Objectives<\/th>\n<th scope=\"col\" style=\"width: 188px;text-align: center\">Magnification<\/th>\n<th scope=\"col\" style=\"width: 188px;text-align: center\">Medium<\/th>\n<th scope=\"col\" style=\"width: 188px;text-align: center\">Working Distance (mm)<\/th>\n<\/tr>\n<tr>\n<td style=\"width: 188px;text-align: center\">EC Plan-Neofluar<\/td>\n<td style=\"width: 188px;text-align: center\">\u00a010x\/0.3<\/td>\n<td style=\"width: 188px;text-align: center\">Air<\/td>\n<td style=\"width: 188px;text-align: center\">\u00a05.2<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 188px;text-align: center\">Plan-Apochromat<\/td>\n<td style=\"width: 188px;text-align: center\">\u00a020x\/0.8<\/td>\n<td style=\"width: 188px;text-align: center\">Air<\/td>\n<td style=\"width: 188px;text-align: center\">\u00a00.55<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 188px;text-align: center\">LD LCI Plan-Apochromat<\/td>\n<td style=\"width: 188px;text-align: center\">\u00a025x\/0.8<\/td>\n<td style=\"width: 188px;text-align: center\">Water, Silicone, Glycerine, Oil<\/td>\n<td style=\"width: 188px;text-align: center\">\u00a00.57<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 188px;text-align: center\">Achrostigmat<\/td>\n<td style=\"width: 188px;text-align: center\">\u00a040x\/1.3<\/td>\n<td style=\"width: 188px;text-align: center\">Oil<\/td>\n<td style=\"width: 188px;text-align: center\">\u00a00.21<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 188px;text-align: center\">C-Apochromat<\/td>\n<td style=\"width: 188px;text-align: center\">\u00a040x\/1.2<\/td>\n<td style=\"width: 188px;text-align: center\">Water<\/td>\n<td style=\"width: 188px;text-align: center\">\u00a00.28<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 188px;text-align: center\">Plan-Apochromat<\/td>\n<td style=\"width: 188px;text-align: center\">63x\/1.4<\/td>\n<td style=\"width: 188px;text-align: center\">Oil<\/td>\n<td style=\"width: 188px;text-align: center\">\u00a00.13<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"pg-287-3\" class=\"panel-grid panel-no-style\">\n<div id=\"pgc-287-3-0\" class=\"panel-grid-cell\" data-weight=\"1\">\n<div id=\"panel-287-3-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"4\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;,&quot;background_image_size&quot;:&quot;full&quot;,&quot;background_image_opacity&quot;:&quot;100&quot;,&quot;border_thickness&quot;:&quot;1px&quot;,&quot;accordion&quot;:&quot;&quot;}\">\n<div class=\"textwidget\">\n<div class=\"panel panel-default\">\n<div class=\"panel-heading\">\n<h3 class=\"panel-title\"><strong>Zeiss Observer Z1 &#8211; TIRF<\/strong><\/h3>\n<\/div>\n<div class=\"panel-body\">\n<p><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/09\/TIRF-Scope-Small.png\" alt=\"Zeiss Observer Z1 microscope in the CCAM lab. \" width=\"160\" height=\"186\" class=\"alignleft wp-image-3948 size-full\" \/><\/p>\n<p>The Zeiss Observer Z1 is a motorized, inverted microscope fitted with a stage-top incubation system for live cell imaging, providing heating, humidification, and carbon dioxide. The system is equipped with a 5.5-megapixel, Andor Neo sCMOS camera. The scope is capable of phase, DIC, and fluorescence microscopy. The Lumencor Spectra 7, 6 LED light source is currently configured for imaging DAPI, GFP, Rhodamine, and Cy5. Objective choices range from 10x through 63x.<\/p>\n<p>An advanced imaging feature of this system is its capability to perform TIRF using 488 nm, 514 nm, and 561 nm wavelengths for fluorescent indicators such as GFP,\u00a0 YFP, and RFP using the 100x\/1.45 alpha-Plan Fluar oil immersion objective.<\/p>\n<p>The microscope is controlled via MicroManager, a plugin from FIJI, enabling it to acquire single-channel or multi-channel images as well as time series with multiple position acquisition.<\/p>\n<figure id=\"attachment_3949\" aria-describedby=\"caption-attachment-3949\" style=\"width: 400px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/09\/Observer-Cells-sm.png\" alt=\"3T3 Cells stained with MitoTracker Red ( purple) and Alexa Fluor 488 phalloidin (green). \" width=\"400\" height=\"285\" class=\"wp-image-3949 size-full\" srcset=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/09\/Observer-Cells-sm.png 400w, https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/09\/Observer-Cells-sm-300x214.png 300w\" sizes=\"(max-width: 400px) 100vw, 400px\" \/><figcaption id=\"caption-attachment-3949\" class=\"wp-caption-text\">3T3 cells stained with MitoTracker Red and Alexa Fluor 488 phalloidin. (CCAM)<\/figcaption><\/figure>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"pg-287-4\" class=\"panel-grid panel-no-style\" data-style=\"{&quot;background_display&quot;:&quot;tile&quot;,&quot;full_height&quot;:&quot;&quot;}\">\n<div id=\"pgc-287-4-0\" class=\"panel-grid-cell\" data-weight=\"1\">\n<div id=\"panel-287-4-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"5\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;,&quot;accordion&quot;:&quot;&quot;}\">\n<div class=\"textwidget\">\n<div class=\"panel panel-default\">\n<div class=\"panel-heading\">\n<h3 class=\"panel-title\"><strong>Imaging01<\/strong><\/h3>\n<\/div>\n<div class=\"panel-body\"><img loading=\"lazy\" decoding=\"async\" class=\"alignleft wp-image-2885 size-full\" src=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2021\/03\/user-terminal.png\" alt=\"User terminal\" width=\"167\" height=\"132\" align=\"middle\" \/>This CCAM user computer is a Windows 7 computer with MetaMorph, Photoshop, Office, Zeiss LSM and Zen Image Browsers. All the software is available, with prior approval, via remote access.<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"pg-287-5\" class=\"panel-grid panel-no-style\" data-style=\"{&quot;background_display&quot;:&quot;tile&quot;,&quot;full_height&quot;:&quot;&quot;}\">\n<div id=\"pgc-287-5-0\" class=\"panel-grid-cell\" data-weight=\"1\">\n<div id=\"panel-287-5-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"6\" data-style=\"{&quot;background_display&quot;:&quot;tile&quot;,&quot;accordion&quot;:&quot;&quot;}\">\n<div class=\"textwidget\">\n<div class=\"line-separator3\"><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"pg-287-6\" class=\"panel-grid panel-no-style\">\n<div id=\"pgc-287-6-0\" class=\"panel-grid-cell\" data-weight=\"0.5\">\n<div id=\"panel-287-6-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"7\" data-style=\"{&quot;background_display&quot;:&quot;tile&quot;,&quot;accordion&quot;:&quot;&quot;}\">\n<div class=\"textwidget\">\n<h2 class=\"panel-heading\"><a name=\"cellbiology\"><\/a>Cell &amp; Genome Sciences Building (CGSB)<br \/>\nImaging Suite R1510<br \/>\n400 Farmington Avenue<\/h2>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"pgc-287-6-1\" class=\"panel-grid-cell\" data-weight=\"0.5\">\n<div id=\"panel-287-6-1-0\" class=\"so-panel widget widget_widget_sp_image widget_sp_image panel-first-child panel-last-child\" data-index=\"8\" data-style=\"{&quot;background_display&quot;:&quot;tile&quot;,&quot;accordion&quot;:&quot;&quot;}\"><\/div>\n<\/div>\n<\/div>\n<div id=\"pg-287-7\" class=\"panel-grid panel-no-style\" data-style=\"{&quot;background_display&quot;:&quot;tile&quot;,&quot;full_height&quot;:&quot;&quot;}\">\n<div id=\"pgc-287-7-0\" class=\"panel-grid-cell\" data-weight=\"1\">\n<div id=\"panel-287-7-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"9\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;,&quot;background_image_size&quot;:&quot;full&quot;,&quot;background_image_opacity&quot;:&quot;100&quot;,&quot;border_thickness&quot;:&quot;1px&quot;,&quot;accordion&quot;:&quot;&quot;}\">\n<div class=\"textwidget\">\n<div class=\"panel panel-default\">\n<div class=\"panel-heading\">\n<h3 class=\"panel-title\"><strong>Zeiss Elyra 7 SIM<sup>2<\/sup> &#8211; R1521<\/strong><\/h3>\n<\/div>\n<div class=\"panel-body\">\n<p><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/07\/Elyra-7-ZeissWebImg.png\" alt=\"View of Zeiss Elyra with Incubation Chamber\" width=\"167\" height=\"132\" class=\"alignleft wp-image-2889 size-full\" \/><\/p>\n<p>Structured illumination microscopy, SIM, is a widefield technique that greatly increases the diffraction-limited resolution of the light microscope, ~ 200 nm, to super-resolution, ~ 100 nm. SIM uses a periodic grating that is projected onto the image plane of the microscope. Software is then used to analyze the resulting Moir\u00e9 pattern, and through deconvolution, a super-resolution image is obtained. \u00a0The Zeiss Elyra 7 SIM<sup>2 <\/sup>uses an advanced reconstruction algorithm that improves the SIM resolution to less than 100 nm, along with quick image acquisition and compatibility for live-cell applications.<br \/>\n&nbsp;<\/p>\n<ul style=\"list-style-type: disc\">\n<li>Zeiss Axio Observer 7, with definite focus<\/li>\n<li>XY and Z piezo-controlled stage and focus<\/li>\n<li>Two CMOS cameras for dual acquisition<\/li>\n<li>Incubation system for temperature, humidity, and carbon dioxide<\/li>\n<li>TIRF, PALM, and STORM.<\/li>\n<li>Samples live and fixed, up to ~ 150 \u00b5m thickness<\/li>\n<\/ul>\n<figure id=\"attachment_3951\" aria-describedby=\"caption-attachment-3951\" style=\"width: 572px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/09\/WF-Apotome-Adjusted-sm-text.png\" alt=\"RPE Choroid Wholemount\" width=\"572\" height=\"285\" class=\"wp-image-3951 size-full\" srcset=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/09\/WF-Apotome-Adjusted-sm-text.png 572w, https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/09\/WF-Apotome-Adjusted-sm-text-300x149.png 300w\" sizes=\"(max-width: 572px) 100vw, 572px\" \/><figcaption id=\"caption-attachment-3951\" class=\"wp-caption-text\">Mouse retina pigments epithelium cells\/choroid wholemount with tight junction (anti- ZO1, Green) and ROS stress levels (anti-8OHdG, purple). (Y. Jiang\/Fong Lab)<\/figcaption><\/figure>\n<p>&nbsp;<\/p>\n<\/div>\n<div class=\"panel-body\">\n<table style=\"height: 166px;width: 752px;margin-left: auto;margin-right: auto\">\n<caption>Zeiss Elyra Available Wavelengths and Associated Fluorophores<\/caption>\n<tbody>\n<tr>\n<th scope=\"col\" style=\"width: 306px;text-align: center\">Laser Wavelengths (nm)<\/th>\n<th scope=\"col\" style=\"width: 432px;text-align: center\">Common Fluorophores<\/th>\n<\/tr>\n<tr>\n<td style=\"width: 306px;text-align: center\">\u00a0405 nm\u00a0 for PALM<\/td>\n<td style=\"width: 432px;text-align: center\"><span>Alexa405, DAPI, Hoechst<\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 306px;text-align: center\">\u00a0488 nm<\/td>\n<td style=\"width: 432px;text-align: center\"><span>Alexa488, GFP<\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 306px;text-align: center\">\u00a0561 nm (500mW)<\/td>\n<td style=\"width: 432px;text-align: center\"><span>Alexa568, Rhodamine<\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 306px;text-align: center\">\u00a0640 nm (500mW)<\/td>\n<td style=\"width: 432px;text-align: center\">Alexa 647, Cy5<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<table style=\"height: 166px;width: 752px;margin-left: auto;margin-right: auto\">\n<caption>Zeiss Elyra Available Objectives<\/caption>\n<tbody>\n<tr>\n<th scope=\"col\" style=\"width: 306px;text-align: center\">Objectives<\/th>\n<th scope=\"col\" style=\"width: 432px;text-align: center\">Magnification<\/th>\n<th scope=\"col\" style=\"width: 432px;text-align: center\">Medium<\/th>\n<th scope=\"col\" style=\"width: 432px;text-align: center\">Working Distance (mm)<\/th>\n<\/tr>\n<tr>\n<td style=\"width: 306px;text-align: center\">EC Plan-Neofluar<\/td>\n<td style=\"width: 306px;text-align: center\">10x\/0.3<\/td>\n<td style=\"width: 306px;text-align: center\">Air<\/td>\n<td style=\"width: 432px;text-align: center\">5.2<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 306px;text-align: center\">Plan-Apochromat<\/td>\n<td style=\"width: 306px;text-align: center\">20x\/0.8<\/td>\n<td style=\"width: 306px;text-align: center\">Air<\/td>\n<td style=\"width: 432px;text-align: center\">\u00a00.55<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 306px;text-align: center\">Plan-Apochromat<\/td>\n<td style=\"width: 306px;text-align: center\">40x\/1.4<\/td>\n<td style=\"width: 306px;text-align: center\">Oil<\/td>\n<td style=\"width: 432px;text-align: center\">\u00a0 0.13<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 306px;text-align: center\">Plan-Apochromat<\/td>\n<td style=\"width: 432px;text-align: center\">\u00a063x\/1.4<\/td>\n<td style=\"width: 306px;text-align: center\">Oil<\/td>\n<td style=\"width: 306px;text-align: center\">0.19<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 306px;text-align: center\">C-Apochromat Corr<\/td>\n<td style=\"width: 432px;text-align: center\">\u00a063x\/1.2<\/td>\n<td style=\"width: 306px;text-align: center\">Water<\/td>\n<td style=\"width: 306px;text-align: center\">0.17<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 306px;text-align: center\">alpha Plan-Apochromat Corr<\/td>\n<td style=\"width: 432px;text-align: center\">\u00a063x\/1.46<\/td>\n<td style=\"width: 306px;text-align: center\">Oil<\/td>\n<td style=\"width: 306px;text-align: center\">0.10<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 306px;text-align: center\">Plan-Apochromat<\/td>\n<td style=\"width: 432px;text-align: center\">100x\/1.57<\/td>\n<td style=\"width: 306px;text-align: center\">Oil-HI<\/td>\n<td style=\"width: 306px;text-align: center\">0.12<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"pg-287-8\" class=\"panel-grid panel-no-style\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;,&quot;background_image_size&quot;:&quot;full&quot;,&quot;background_image_opacity&quot;:&quot;100&quot;,&quot;border_thickness&quot;:&quot;1px&quot;,&quot;full_height&quot;:&quot;&quot;,&quot;cell_alignment&quot;:&quot;flex-start&quot;}\">\n<div id=\"pgc-287-8-0\" class=\"panel-grid-cell\" data-weight=\"1\">\n<div id=\"panel-287-8-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"10\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;,&quot;background_image_size&quot;:&quot;full&quot;,&quot;background_image_opacity&quot;:&quot;100&quot;,&quot;border_thickness&quot;:&quot;1px&quot;,&quot;accordion&quot;:&quot;&quot;}\">\n<div class=\"textwidget\">\n<div class=\"panel panel-default\">\n<div class=\"panel-heading\">\n<h3 class=\"panel-title\"><strong>Nikon Crest V3 Spinning Disk Confocal &#8211; R1522<\/strong><\/h3>\n<\/div>\n<div class=\"panel-body\">\n<p><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2025\/01\/NikonSDCropped.png\" alt=\"CCAM's Nikon Spinning Disk Confocal\" width=\"227\" height=\"173\" class=\"alignleft wp-image-4423\" srcset=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2025\/01\/NikonSDCropped.png 1903w, https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2025\/01\/NikonSDCropped-300x229.png 300w, https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2025\/01\/NikonSDCropped-1024x781.png 1024w, https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2025\/01\/NikonSDCropped-768x586.png 768w, https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2025\/01\/NikonSDCropped-1536x1171.png 1536w\" sizes=\"(max-width: 227px) 100vw, 227px\" \/><\/p>\n<ul style=\"list-style-type: square\">\n<li style=\"text-align: left\">Confocal and Widefield Modes for samples not exceeding 50 um<\/li>\n<li style=\"text-align: left\">X-Light V3 Spinning Disk by CrestOptics<\/li>\n<li style=\"text-align: left\"><span>70 um pinhole size, 250 um spacing<\/span><\/li>\n<li style=\"text-align: left\">Fast Imaging,\u00a0 and Tiling Capabilities,\u00a0 with Large Field of View, 25mm<\/li>\n<li><span>\u00a0Nikon Eclipse Ti2 with Perfect Focus System, and <\/span>XY Motorized Stage<\/li>\n<li><span>Teledyne Photometrics Kinetix 10MP sCMOS Camera<\/span><\/li>\n<li><span>Tokai Hit Stage Top Incubation System with CO2 and O2<\/span><\/li>\n<\/ul>\n<h3 class=\"centered\">Waveform Analysis of <i>Chlamydomonas<\/i> Using High Speed Acquisition Settings<\/h3>\n<div style=\"width: 640px;\" class=\"wp-video\"><!--[if lt IE 9]><script>document.createElement('video');<\/script><![endif]-->\n<video class=\"wp-video-shortcode\" id=\"video-287-1\" width=\"640\" height=\"320\" poster=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2025\/04\/SpeedAcquisitionFirstFrame.png\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2025\/04\/SpeedAcquisitionV2.mp4?_=1\" \/><a href=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2025\/04\/SpeedAcquisitionV2.mp4\">https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2025\/04\/SpeedAcquisitionV2.mp4<\/a><\/video><\/div>\n<\/div>\n<div class=\"panel-body\">\n<table style=\"height: 166px;width: 752px;margin-left: auto;margin-right: auto\">\n<caption>Nikon Crest System Configuration and Associated Fluorophores<\/caption>\n<tbody>\n<tr>\n<th scope=\"col\" style=\"width: 306px;text-align: center\">Lasers<\/th>\n<th scope=\"col\" style=\"width: 432px;text-align: center\">Emission Filters<\/th>\n<th scope=\"col\" style=\"width: 432px;text-align: center\">Common Fluorophores<\/th>\n<\/tr>\n<tr>\n<td style=\"width: 306px;text-align: center\">\u00a0405 nm<\/td>\n<td style=\"width: 306px;text-align: center\">\u00a0438\/24 (426 &#8211; 450)<\/td>\n<td style=\"width: 432px;text-align: center\">DAPI<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 306px;text-align: center\">\u00a0440 nm<\/td>\n<td style=\"width: 306px;text-align: center\">\u00a0485\/20 (475 &#8211; 495)<\/td>\n<td style=\"width: 432px;text-align: center\">\u00a0CFP<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 306px;text-align: center\">\u00a0477 nm<\/td>\n<td style=\"width: 306px;text-align: center\">\u00a0511\/20 (501 &#8211; 521)<\/td>\n<td style=\"width: 432px;text-align: center\">GFP, Alexa 488<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 306px;text-align: center\">\u00a0518 nm<\/td>\n<td style=\"width: 306px;text-align: center\">\u00a0560\/25 (548 &#8211; 572)<\/td>\n<td style=\"width: 432px;text-align: center\">\u00a0YFP<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 306px;text-align: center\">545 nm<\/td>\n<td style=\"width: 306px;text-align: center\">\u00a0595\/31 (580 &#8211; 610)<\/td>\n<td style=\"width: 432px;text-align: center\">CY3, Alexa 555<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 306px;text-align: center\">639 nm<\/td>\n<td style=\"width: 306px;text-align: center\">\u00a0685\/40 (665 &#8211; 705)<\/td>\n<td style=\"width: 432px;text-align: center\">CY5, Alexa 647<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 306px;text-align: center\">745 nm<\/td>\n<td style=\"width: 306px;text-align: center\">\u00a0794\/32 (778 &#8211; 810)<\/td>\n<td style=\"width: 432px;text-align: center\">CY7, Alexa 750<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<table style=\"height: 240px;width: 752px;margin-left: auto;margin-right: auto\">\n<caption>Nikon Crest Available Objectives<\/caption>\n<tbody>\n<tr style=\"height: 48px\">\n<th scope=\"col\" style=\"width: 200px;text-align: center;height: 48px\">Objectives<\/th>\n<th scope=\"col\" style=\"width: 184px;text-align: center;height: 48px\">Magnification<\/th>\n<th scope=\"col\" style=\"width: 184px;text-align: center;height: 48px\">Medium<\/th>\n<th scope=\"col\" style=\"width: 184px;text-align: center;height: 48px\">Working Distance (mm)<\/th>\n<\/tr>\n<tr style=\"height: 24px\">\n<td style=\"width: 200px;text-align: center;height: 24px\">Plan-Apochromat \u03bb D<\/td>\n<td style=\"width: 184px;text-align: center;height: 24px\">10x\/0.45<\/td>\n<td style=\"width: 184px;text-align: center;height: 24px\">\u00a0Air<\/td>\n<td style=\"width: 184px;text-align: center;height: 24px\">\u00a04.0<\/td>\n<\/tr>\n<tr style=\"height: 24px\">\n<td style=\"width: 200px;text-align: center;height: 24px\">Plan-Apochromat \u03bb D<\/td>\n<td style=\"width: 184px;text-align: center;height: 24px\">\u00a020x\/0.8<\/td>\n<td style=\"width: 184px;text-align: center;height: 24px\">Air<\/td>\n<td style=\"width: 184px;text-align: center;height: 24px\">\u00a00.8<\/td>\n<\/tr>\n<tr style=\"height: 48px\">\n<td style=\"width: 200px;text-align: center;height: 48px\">Plan-Apochromat \u03bb S\u00a0 CORR<\/td>\n<td style=\"width: 184px;text-align: center;height: 48px\">40x\/1.25<\/td>\n<td style=\"width: 184px;text-align: center;height: 48px\">Silicone<\/td>\n<td style=\"width: 184px;text-align: center;height: 48px\">\u00a00.3<\/td>\n<\/tr>\n<tr style=\"height: 24px\">\n<td style=\"width: 200px;text-align: center;height: 24px\">\u00a0 Plan-Apochromat \u03bb D<\/td>\n<td style=\"width: 184px;text-align: center;height: 24px\">60x\/1.42<\/td>\n<td style=\"width: 184px;text-align: center;height: 24px\">\u00a0Oil<\/td>\n<td style=\"width: 184px;text-align: center;height: 24px\">\u00a00.15<\/td>\n<\/tr>\n<tr style=\"height: 24px\">\n<td style=\"width: 200px;text-align: center;height: 24px\">\u00a0Plan-Apochromat Ph3<\/td>\n<td style=\"width: 184px;text-align: center;height: 24px\">60x\/1.40<\/td>\n<td style=\"width: 184px;text-align: center;height: 24px\">\u00a0 Oil<\/td>\n<td style=\"width: 184px;text-align: center;height: 24px\">0.16 &#8211; 0.18<\/td>\n<\/tr>\n<tr style=\"height: 24px\">\n<td style=\"width: 200px;text-align: center;height: 24px\">\u00a0Plan-Apochromat \u03bb D<\/td>\n<td style=\"width: 184px;text-align: center;height: 24px\">100x\/1.45<\/td>\n<td style=\"width: 184px;text-align: center;height: 24px\">\u00a0 Oil<\/td>\n<td style=\"width: 184px;text-align: center;height: 24px\">\u00a00.13<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"pg-287-9\" class=\"panel-grid panel-no-style\" data-style=\"{&quot;background_display&quot;:&quot;tile&quot;,&quot;full_height&quot;:&quot;&quot;}\">\n<div id=\"pgc-287-9-0\" class=\"panel-grid-cell\" data-weight=\"1\">\n<div id=\"panel-287-9-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"11\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;,&quot;background_image_size&quot;:&quot;full&quot;,&quot;background_image_opacity&quot;:&quot;100&quot;,&quot;border_thickness&quot;:&quot;1px&quot;,&quot;accordion&quot;:&quot;&quot;}\">\n<div class=\"textwidget\">\n<div class=\"panel panel-default\">\n<div class=\"panel-heading\">\n<h3 class=\"panel-title\"><strong>Zeiss 780 &#8211; R1523<\/strong><\/h3>\n<\/div>\n<div class=\"panel-body\">\n<p><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/07\/780-Zeiss-LSM-WebImg.png\" alt=\"Zeiss LSM 780\" width=\"164\" height=\"190\" class=\"alignleft size-full wp-image-3774\" \/><\/p>\n<ul>\n<li style=\"list-style-type: none\">\n<ul style=\"list-style-type: disc\">\n<li>confocal\/FCS\/NLO system<\/li>\n<li>Axio Observer Z1.<\/li>\n<li>Spectral 32-channel QUASAR GaAsp Detector allowing for spectral imaging, maximum sensitivity photon counting, and Fluorescence Correlation Spectroscopy (FCS) as well as two adjacent PMTs.<\/li>\n<li>GaAsp detector\u00a0 with range of 410 &#8211; 696 nm<\/li>\n<li>Two PMTs with a range from 415 &#8211; 735 nm.<\/li>\n<li>Incubation system for temperature, humidity and carbon dioxide control.<\/li>\n<li>Becker-Hinkl Fluorescence Lifetime Imaging Microscopy (FLIM) detector on a non-de-scanned detection port, particularly useful for detection of fluorescence resonance energy transfer (FRET) based probes.<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<table style=\"height: 166px;width: 95%;margin-left: auto;margin-right: auto\">\n<caption>Zeiss 780 LSM System Configuration and Associated Fluorophores<\/caption>\n<tbody>\n<tr>\n<th scope=\"col\" style=\"width: 306px;text-align: center\">Lasers<\/th>\n<th scope=\"col\" style=\"width: 432px;text-align: center\">Type<\/th>\n<th scope=\"col\" style=\"width: 432px;text-align: center\">Common Fluorophores<\/th>\n<\/tr>\n<tr>\n<td style=\"width: 306px;text-align: center\">\u00a0405 nm<\/td>\n<td style=\"width: 432px;text-align: center\">Diode<\/td>\n<td style=\"width: 432px;text-align: center\"><span>Alexa405, DAPI, Hoechst<\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 306px;text-align: center\">\u00a0440 nm<\/td>\n<td style=\"width: 432px;text-align: center\">Diode<\/td>\n<td style=\"width: 432px;text-align: center\"><span>LSSmOrange, Sapphire<\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 306px;text-align: center\">\u00a0458, 488, and 514 nm<\/td>\n<td style=\"width: 432px;text-align: center\">\u00a0Argon<\/td>\n<td style=\"width: 432px;text-align: center\">\u00a0CFP, Alexa 488, YFP<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 306px;text-align: center\">\u00a0561 nm<\/td>\n<td style=\"width: 432px;text-align: center\">Diode Pumped Solid State (DPSS)<\/td>\n<td style=\"width: 432px;text-align: center\">Alexa 555, Cy3<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 306px;text-align: center\">\u00a0633 nm<\/td>\n<td style=\"width: 432px;text-align: center\">Helium Neon (HeNe)<\/td>\n<td style=\"width: 432px;text-align: center\">Alexa 647, Cy5<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 306px;text-align: center\">720 &#8211; 950 nm<\/td>\n<td style=\"width: 432px;text-align: center\">Coherent Chameleon tunable, software controlled, mode locked Ti:Sapphire laser for multi-photon excitation (NLO)<\/td>\n<td style=\"width: 432px;text-align: center\">DAPI 740 nm, GFP 850 nm<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<table style=\"height: 166px;width: 95%;margin-left: auto;margin-right: auto\">\n<caption>Zeiss 780 LSM Available Objectives<\/caption>\n<tbody>\n<tr>\n<th scope=\"col\" style=\"width: 26.6845%;text-align: center\">Objectives<\/th>\n<th scope=\"col\" style=\"width: 25.9573%;text-align: center\">Magnification<\/th>\n<th scope=\"col\" style=\"width: 51.4533%;text-align: center\">Medium<\/th>\n<th scope=\"col\" style=\"width: 110.779%;text-align: center\">Working Distance (mm)<\/th>\n<\/tr>\n<tr>\n<td style=\"width: 26.6845%;text-align: center\">C-Apochromat<\/td>\n<td style=\"width: 25.9573%;text-align: center\">\u00a010x\/0.45 W<\/td>\n<td style=\"width: 51.4533%;text-align: center\">Water<\/td>\n<td style=\"width: 110.779%;text-align: center\">1.8<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 26.6845%;text-align: center\">Plan-Apochromat<\/td>\n<td style=\"width: 25.9573%;text-align: center\">\u00a020x\/0.8<\/td>\n<td style=\"width: 51.4533%;text-align: center\">Air<\/td>\n<td style=\"width: 110.779%;text-align: center\">.55<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 26.6845%;text-align: center\">C-Apochromat<\/td>\n<td style=\"width: 25.9573%;text-align: center\">\u00a040x\/1.2 W<\/td>\n<td style=\"width: 51.4533%;text-align: center\">Water<\/td>\n<td style=\"width: 110.779%;text-align: center\">.41<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 26.6845%;text-align: center\">Plan-Neofluar<\/td>\n<td style=\"width: 25.9573%;text-align: center\">\u00a040x\/1.3<\/td>\n<td style=\"width: 51.4533%;text-align: center\">Oil<\/td>\n<td style=\"width: 110.779%;text-align: center\">.21<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 26.6845%;text-align: center\">\u00a0Plan-Apochromat<\/td>\n<td style=\"width: 25.9573%;text-align: center\">\u00a063x\/1.4<\/td>\n<td style=\"width: 51.4533%;text-align: center\">Oil<\/td>\n<td style=\"width: 110.779%;text-align: center\">.19<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"pg-287-10\" class=\"panel-grid panel-no-style\" data-style=\"{&quot;background_display&quot;:&quot;tile&quot;,&quot;full_height&quot;:&quot;&quot;}\">\n<div id=\"pgc-287-10-0\" class=\"panel-grid-cell\" data-weight=\"1\">\n<div id=\"panel-287-10-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"12\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;,&quot;accordion&quot;:&quot;&quot;}\">\n<div class=\"textwidget\">\n<div class=\"panel panel-default\">\n<div class=\"panel-heading\">\n<h3 class=\"panel-title\"><strong>Collaborative PALM and diSPIM<\/strong><\/h3>\n<\/div>\n<div class=\"panel-body\">\n<p><strong>Photo-Activated Localization Microscopy (PALM)<\/strong> super-resolution light microscopy is available through collaboration using a home-built, fully automated PALM imaging system in a CCAM associated laboratory. This system is built on ASI\u2019s custom Rapid Automated Modular Mounting (RAMM) platform, uses 440, 515 and 594 nm (Coherent OBIS) laser lines, and is equipped with Hamamatsu sCMOS cameras for image acquisition.<\/p>\n<p><strong>diSPIM (dual-view inverted Selective Plane Illumination Microscope)<\/strong> imaging system from ASI imaging Inc is available through collaboration in a CCAM associated laboratory for lightsheet imaging on the cellular as opposed to tissue scale.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"pg-287-11\" class=\"panel-grid panel-no-style\">\n<div id=\"pgc-287-11-0\" class=\"panel-grid-cell\" data-weight=\"1\">\n<div id=\"panel-287-11-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"13\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;,&quot;background_image_size&quot;:&quot;full&quot;,&quot;background_image_opacity&quot;:&quot;100&quot;,&quot;border_thickness&quot;:&quot;1px&quot;,&quot;accordion&quot;:&quot;&quot;}\">\n<div class=\"textwidget\">\n<div class=\"panel panel-default\">\n<div class=\"panel-heading\">\n<h3 class=\"panel-title\"><strong>Zeiss LightSheet &#8211; R1515<\/strong><\/h3>\n<\/div>\n<div class=\"panel-body\">\n<p><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/07\/LightSheet-WebImg-B.png\" alt=\"Zeiss LightSheet Z1\" width=\"200\" height=\"127\" class=\"alignleft size-full wp-image-3773\" \/> The Zeiss LightSheet is an advanced fluorescent microscope that allows you to image optical sections of large, live or fixed samples with virtually no bleaching or phototoxicity to the specimen.\u00a0 <span>The optics are equipped for water-based or cleared specimens and dual sCMOS, cooled cameras to provide high sensitivity, speed, 3-D imaging of live or fixed thick specimens. <\/span>The extremely fast acquisition speeds allow you to capture large amounts of data within a fraction of the time compared to other imaging techniques.<\/p>\n<table style=\"height: 166px;width: 752px;margin-left: auto;margin-right: auto\">\n<caption>Zeiss Lightsheet System Configuration<\/caption>\n<tbody>\n<tr>\n<th scope=\"col\" style=\"width: 306px;text-align: center\">Lasers<\/th>\n<th scope=\"col\" style=\"width: 432px;text-align: center\">Emission Objectives<\/th>\n<th scope=\"col\" style=\"width: 432px;text-align: center\">Excitation Objectives<\/th>\n<\/tr>\n<tr>\n<td style=\"width: 306px;text-align: center\">\u00a0405 nm Diode<\/td>\n<td style=\"width: 432px;text-align: center\">2.5x\/0.12 Fluar<\/td>\n<td style=\"width: 432px;text-align: center\">5x\/0.1<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 306px;text-align: center\">\u00a0488\u00a0nm Diode<\/td>\n<td style=\"width: 432px;text-align: center\">5x\/0.16 EC Plan-Neofluar<\/td>\n<td style=\"width: 432px;text-align: center\">5x\/0.1<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 306px;text-align: center\">\u00a0561 nm\u00a0\u00a0Diode<\/td>\n<td style=\"width: 432px;text-align: center\">20x\/1.0 Plan-Neofluar Clearing<\/td>\n<td style=\"width: 432px;text-align: center\">10x\/0.2<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 306px;text-align: center\">\u00a0633 nm\u00a0Diode<\/td>\n<td style=\"width: 432px;text-align: center\">20x\/1.0 Plan-Apochromat Water<\/td>\n<td style=\"width: 432px;text-align: center\">10x\/0.2<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>The Translucene Biosystems imaging chamber, along with the 2.5x objective, allows for larger samples, and an ease for mounting samples. This chamber is also compatible with the 5x objective and can be used for imaging samples in higher refractive index buffers.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2024\/05\/ImaginChamber.png\" width=\"431\" height=\"195\" alt=\"lightsheet\" class=\"alignleft\" \/><\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"pg-287-12\" class=\"panel-grid panel-no-style\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;,&quot;full_height&quot;:&quot;&quot;,&quot;cell_alignment&quot;:&quot;flex-start&quot;}\">\n<div id=\"pgc-287-12-0\" class=\"panel-grid-cell\" data-weight=\"1\">\n<div id=\"panel-287-12-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"14\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;,&quot;background_image_size&quot;:&quot;full&quot;,&quot;background_image_opacity&quot;:&quot;100&quot;,&quot;border_thickness&quot;:&quot;1px&quot;,&quot;accordion&quot;:&quot;&quot;}\">\n<div class=\"textwidget\">\n<div class=\"panel panel-default\">\n<div class=\"panel-heading\">\n<h3 class=\"panel-title\"><strong>Zeiss AxioVert 200M &#8211; R1522<\/strong><\/h3>\n<\/div>\n<div class=\"panel-body\">\n<p><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/07\/AxioVert200M-WebImg-.png\" alt=\"Partial view of a tissue culture hood. \" width=\"160\" height=\"186\" class=\"alignleft size-full wp-image-3776\" \/>The Zeiss AxioVert 200M is a motorized, inverted microscope fitted with an incubation system for live cell imaging, providing heating, humidification, and carbon dioxide environmental conditions. The system is equipped with a pco.edge 4.2 sCMOS camera, with 95% quantum efficiency at 580nm, and 2048 (H) x 2048 (V) pixels.\u00a0 The maximum from rate is 40 fps at full resolution. The scope is capable of phase and DIC microscopy and is fitted with a Lumencor Spectra 7, 6 LED light source for imaging with CFP, GFP, OFP\/mCherry, and Cy5. MetaMorph software is used for the acquisition and analysis of data, including the ability to capture long-term, time series with multiple positions.<\/p>\n<p style=\"text-align: center\"><a href=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/03\/3T3-Cells-3-Day.mp4\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/03\/3T3_Cells_3_Day_First_Frame-300x229.png\" alt=\"4 Panel transmitted light 3T3 cells\" width=\"300\" height=\"229\" class=\"wp-image-3693 size-medium aligncenter\" srcset=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/03\/3T3_Cells_3_Day_First_Frame-300x229.png 300w, https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2023\/03\/3T3_Cells_3_Day_First_Frame.png 672w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/a><br \/>\n(When clicked, the video will open a new window. Duration: 43 seconds, Size: 11 MB. )<\/p>\n<h4 style=\"text-align: left\">3 Day experiment, captured on this setup, observing 3T3 cells, in four different positions, was visualized using transmitted light and maintained with 5% carbon dioxide and 37-degree environmental conditions.<\/h4>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"pg-287-13\" class=\"panel-grid panel-no-style\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;,&quot;full_height&quot;:&quot;&quot;,&quot;cell_alignment&quot;:&quot;flex-start&quot;}\">\n<div id=\"pgc-287-13-0\" class=\"panel-grid-cell\" data-weight=\"1\">\n<div id=\"panel-287-13-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"15\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;,&quot;background_image_size&quot;:&quot;full&quot;,&quot;background_image_opacity&quot;:&quot;100&quot;,&quot;border_thickness&quot;:&quot;1px&quot;,&quot;accordion&quot;:&quot;&quot;}\">\n<div class=\"textwidget\">\n<div class=\"panel panel-default\">\n<div class=\"panel-heading\">\n<h3 class=\"panel-title\"><strong>Imaris &#8211; R1510<\/strong><\/h3>\n<\/div>\n<div class=\"panel-body\"><img loading=\"lazy\" decoding=\"async\" class=\"alignleft wp-image-2887 size-full\" src=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2021\/03\/user-terminal-imaris.png\" alt=\"Imaris User Terminal \" width=\"167\" height=\"132\" align=\"middle\" \/>Imaris is a 64 bit computer with ImarisSurpass 3-D rendering and analysis software, MetaMorph, Photoshop, and Zeiss Zen Image Browser.<\/div>\n<div>All software is available, with prior approval,\u00a0 via remote access.<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"pg-287-14\" class=\"panel-grid panel-no-style\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;,&quot;background_image_size&quot;:&quot;full&quot;,&quot;background_image_opacity&quot;:&quot;100&quot;,&quot;border_thickness&quot;:&quot;1px&quot;,&quot;full_height&quot;:&quot;&quot;,&quot;cell_alignment&quot;:&quot;flex-start&quot;}\">\n<div id=\"pgc-287-14-0\" class=\"panel-grid-cell\" data-weight=\"1\">\n<div id=\"panel-287-14-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"16\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;,&quot;background_image_size&quot;:&quot;full&quot;,&quot;background_image_opacity&quot;:&quot;100&quot;,&quot;border_thickness&quot;:&quot;1px&quot;,&quot;accordion&quot;:&quot;&quot;}\">\n<div class=\"textwidget\">\n<div class=\"panel panel-default\">\n<div class=\"panel-heading\">\n<h3 class=\"panel-title\"><strong>Fluorolog &#8211; R1513<\/strong><\/h3>\n<\/div>\n<div class=\"panel-body\"><img loading=\"lazy\" decoding=\"async\" class=\"alignleft wp-image-4047 \" src=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2024\/01\/Spex.png\" alt=\"Spectrofluorometer\" width=\"640\" height=\"214\" srcset=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2024\/01\/Spex.png 4013w, https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2024\/01\/Spex-300x100.png 300w, https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2024\/01\/Spex-1024x342.png 1024w, https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2024\/01\/Spex-768x257.png 768w, https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2024\/01\/Spex-1536x514.png 1536w, https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2024\/01\/Spex-2048x685.png 2048w\" sizes=\"(max-width: 640px) 100vw, 640px\" \/><\/div>\n<div><\/div>\n<div><\/div>\n<div><\/div>\n<div><\/div>\n<div><\/div>\n<div><\/div>\n<div><\/div>\n<div><\/div>\n<div><\/div>\n<div class=\"panel-body\">The Horiba-Jobin Yvon Fluorolog is a dual-emission spectrofluorometer. The visible detector has an emission range of 290 nm &#8211; 850 nm while the IR detector has an emission range of 850 nm &#8211; 1600 nm. The system is capable of emission scans, where the excitation wavelength is fixed, while the emission monochromator scans the sample, and excitation scans, where the emission wavelength is fixed, while the excitation monochromator changes the incident light. The sample chamber can also be temperature-controlled.<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"pg-287-15\" class=\"panel-grid panel-no-style\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;,&quot;background_image_size&quot;:&quot;full&quot;,&quot;background_image_opacity&quot;:&quot;100&quot;,&quot;border_thickness&quot;:&quot;1px&quot;,&quot;full_height&quot;:&quot;&quot;,&quot;cell_alignment&quot;:&quot;flex-start&quot;}\">\n<div id=\"pgc-287-15-0\" class=\"panel-grid-cell\" data-weight=\"1\">\n<div id=\"panel-287-15-0-0\" class=\"so-panel widget widget_black-studio-tinymce widget_black_studio_tinymce panel-first-child panel-last-child\" data-index=\"17\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;,&quot;background_image_size&quot;:&quot;full&quot;,&quot;background_image_opacity&quot;:&quot;100&quot;,&quot;border_thickness&quot;:&quot;1px&quot;,&quot;accordion&quot;:&quot;&quot;}\">\n<div class=\"textwidget\">\n<div class=\"panel panel-default\">\n<div class=\"panel-heading\">\n<h3 class=\"panel-title\"><strong>CCAM Wet Lab &#8211; R1517<\/strong><\/h3>\n<\/div>\n<div class=\"panel-body\"><img loading=\"lazy\" decoding=\"async\" class=\"alignleft wp-image-2889 size-full\" src=\"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-content\/uploads\/sites\/149\/2021\/03\/TissueCultureHood-small.png\" alt=\"Partial view of a tissue culture hood. \" width=\"167\" height=\"132\" align=\"middle\" \/>Provides tissue culture hood and tissue culture microscope, incubators, refrigeration, and bench space.<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Academic Research Building (ARB) E6011 263 Farmington Avenue Please refer to the following spectral tools below to see if your probes are compatible with our equipment. If you need further assistance, please contact Susan, staurovsky@uchc.edu, 860-679-4686. ThermoFisher Scientific Fluorescence SpecrtraViewer (opens in new tab) BioLegend Fluorescence Spectra Analyzer (opens in new tab) FPbase Spectra Viewer [&hellip;]<\/p>\n","protected":false},"author":283,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"acf":[],"publishpress_future_action":{"enabled":false,"date":"2026-05-01 09:38:25","action":"change-status","newStatus":"draft","terms":[],"taxonomy":""},"_links":{"self":[{"href":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-json\/wp\/v2\/pages\/287"}],"collection":[{"href":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-json\/wp\/v2\/users\/283"}],"replies":[{"embeddable":true,"href":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-json\/wp\/v2\/comments?post=287"}],"version-history":[{"count":96,"href":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-json\/wp\/v2\/pages\/287\/revisions"}],"predecessor-version":[{"id":4953,"href":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-json\/wp\/v2\/pages\/287\/revisions\/4953"}],"wp:attachment":[{"href":"https:\/\/health.uconn.edu\/cell-analysis-modeling\/wp-json\/wp\/v2\/media?parent=287"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}